FastQCFastQC Report
Fri 10 Feb 2017
ERR1630659.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630659.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1426460
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36290.2544060120858629No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29840.2091891816104202No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27430.19229421084362688No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC24200.16965074379933542No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT21860.15324649832452364No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA18840.13207520715617682No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA18620.13053292766709196No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16670.11686272310474882No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG15180.10641728474685586No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG14840.10403376190008833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG806.962546E-720.81255
GCTTTAT6750.019.1851861
ACGCTAA609.238306E-418.534
TATTGAG7250.017.3517255
CGAACTA752.067707E-417.26666624
CAAGACG3850.016.3376644
GGACCGT803.3832804E-416.18756
GGTATCA20550.016.1143551
CGAACGA1151.2426317E-616.08695616
AAGACGG3250.015.9384625
TAGACTC1059.346173E-615.8571445
ACGGACC3400.015.7794118
GTAAACG2201.8189894E-1215.13636427
TTTATTG9200.015.0815223
CGCAAGA3600.014.9027792
GCGCAAG3850.014.8961051
TAGCGCA750.004105051514.84
TTGCGTT750.004105051514.84
TATCGCT750.004105051514.87
GTCTAAA1405.9972444E-714.5357151
Produced by FastQC (version 0.11.5)