##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630659.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1426460
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.997979613869298	33.0	31.0	34.0	30.0	34.0
2	32.164898419864556	33.0	31.0	34.0	30.0	34.0
3	32.27470801845127	34.0	31.0	34.0	30.0	34.0
4	35.85352200552416	37.0	35.0	37.0	35.0	37.0
5	35.828145198603536	37.0	35.0	37.0	35.0	37.0
6	35.86256747472765	37.0	35.0	37.0	35.0	37.0
7	35.83962256214685	37.0	35.0	37.0	35.0	37.0
8	35.83342329963686	37.0	35.0	37.0	35.0	37.0
9	37.563581172973656	39.0	37.0	39.0	35.0	39.0
10	37.47105842435119	39.0	37.0	39.0	35.0	39.0
11	37.57390883726147	39.0	37.0	39.0	35.0	39.0
12	37.501850034350774	39.0	37.0	39.0	35.0	39.0
13	37.54951067678028	39.0	37.0	39.0	35.0	39.0
14	38.817781781473016	40.0	38.0	41.0	35.0	41.0
15	38.79801466567587	40.0	38.0	41.0	35.0	41.0
16	38.777497441218124	40.0	38.0	41.0	35.0	41.0
17	38.76303156064664	40.0	38.0	41.0	35.0	41.0
18	38.75511335754245	40.0	38.0	41.0	35.0	41.0
19	38.76094247297506	40.0	38.0	41.0	35.0	41.0
20	38.74882716655216	40.0	38.0	41.0	35.0	41.0
21	38.67173562525412	40.0	38.0	41.0	34.0	41.0
22	38.61781963742411	40.0	38.0	41.0	34.0	41.0
23	38.60171403334128	40.0	38.0	41.0	34.0	41.0
24	38.54616042510831	40.0	38.0	41.0	34.0	41.0
25	38.50249288448327	40.0	38.0	41.0	34.0	41.0
26	38.32107244507382	40.0	38.0	41.0	34.0	41.0
27	38.20417957741542	40.0	38.0	41.0	33.0	41.0
28	38.101771518304055	40.0	38.0	41.0	33.0	41.0
29	38.01333510929153	40.0	37.0	41.0	33.0	41.0
30	37.94778051960798	40.0	37.0	41.0	33.0	41.0
31	37.90623571638882	40.0	37.0	41.0	33.0	41.0
32	37.819460763007726	40.0	37.0	41.0	33.0	41.0
33	37.7818859274007	40.0	37.0	41.0	33.0	41.0
34	37.71355032738387	40.0	37.0	41.0	33.0	41.0
35	37.654218835438776	40.0	37.0	41.0	33.0	41.0
36	37.61081768854367	40.0	37.0	41.0	33.0	41.0
37	37.43099981773061	40.0	36.0	41.0	32.0	41.0
38	37.329649622141524	40.0	36.0	41.0	31.0	41.0
39	37.26761283176535	40.0	36.0	41.0	31.0	41.0
40	37.15419079399352	39.0	36.0	41.0	31.0	41.0
41	37.0425725221878	39.0	36.0	41.0	31.0	41.0
42	36.95441863073623	39.0	35.0	41.0	31.0	41.0
43	36.05618243764284	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	2.0
12	0.0
13	0.0
14	6.0
15	3.0
16	7.0
17	13.0
18	42.0
19	83.0
20	147.0
21	364.0
22	690.0
23	1136.0
24	1979.0
25	3076.0
26	4972.0
27	7255.0
28	10573.0
29	14898.0
30	20029.0
31	26593.0
32	34863.0
33	45523.0
34	60586.0
35	81974.0
36	115514.0
37	177919.0
38	326638.0
39	491571.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.44632166341853	18.35179395146026	11.582238548574795	25.619645836546418
2	20.137823703433675	20.270880361173816	32.41689216662227	27.174403768770244
3	21.865246834821868	22.247451733662352	27.789492870462546	28.097808561053238
4	15.231762545041573	14.46167435469624	33.05210100528581	37.254462094976375
5	15.461702396141497	35.83710724450738	33.470970093798634	15.230220265552486
6	37.13367356953577	32.66716206553286	14.701008089956957	15.498156274974411
7	30.36790376175988	28.918161042020106	20.5709939290271	20.14294126719291
8	28.93891171150961	30.641167645780463	19.542503820646917	20.877416822063008
9	27.27493235001332	13.378853946132383	19.254307867027467	40.09190583682683
10	18.526842673471393	25.507620262748343	31.415111534848506	24.55042552893176
11	36.98792815781725	20.97542167323304	20.25664932770635	21.780000841243357
12	23.911150680706083	24.50576952736144	26.870925227486225	24.71215456444625
13	31.55594969364721	18.77346718449869	24.00515962592712	25.66542349592698
14	23.939051918735892	20.130042202375108	23.771644490557044	32.159261388331956
15	27.121545644462515	26.696998163285336	21.195827432945894	24.985628759306255
16	26.52061747262454	25.106697699199415	22.99412531721885	25.378559510957192
17	25.27915258752436	25.049913772555836	23.883249442676274	25.787684197243525
18	24.846893708901757	23.52396842533264	25.912468628633118	25.716669237132482
19	25.887862260420903	23.985250199795296	25.49745523884301	24.629432300940792
20	26.557912594815136	23.274399562553455	24.742369221709687	25.425318620921722
21	26.605162430071644	23.634451719641632	24.765152895980258	24.995232954306466
22	26.560366221275046	23.750052577709855	24.849277231748523	24.840303969266575
23	25.653295570853725	23.755170141469094	24.66069851240133	25.930835775275856
24	25.430576391907238	24.264122372867096	25.169790951025618	25.135510284200045
25	25.755787053264722	24.255569732063993	24.434754567250398	25.553888647420887
26	25.629951067678032	24.62389411550271	24.727647462950237	25.01850735386902
27	25.970654627539503	23.460174137375038	24.790039678645037	25.779131556440422
28	25.10431417635265	24.18630736228145	24.948263533502516	25.76111492786338
29	25.165654837850344	24.618496137290915	25.3236683818684	24.89218064299034
30	25.2476760652244	24.617304375867533	24.776579784922113	25.358439773985953
31	24.81352438904701	25.228467675223982	24.900452869340885	25.057555066388122
32	24.74888885773173	24.215049843668943	24.741598081965144	26.294463216634185
33	24.190233164617304	24.79768097247732	25.247605961611264	25.764479901294113
34	26.029121040898445	23.529366403544437	24.94258514083816	25.498927414718953
35	25.021591912847192	23.868317373077407	25.407512303184106	25.7025784108913
36	24.236922170968693	25.010515541971035	25.44067131219943	25.311890974860844
37	25.173296131682626	23.71703377592081	25.7803233178638	25.32934677453276
38	24.909566339049114	23.981464604685726	25.56117942318747	25.547789633077688
39	24.911599343830183	23.525019979529745	25.84285574078488	25.720524935855195
40	23.984759474503317	23.470619575732936	26.47294701568919	26.071673934074564
41	23.128513943608652	23.914936275815656	27.045693535044794	25.910856245530894
42	24.013782370343367	23.601362814239447	27.42944071337437	24.95541410204282
43	24.04960531665802	23.172328701821293	26.842182746098736	25.935883235421954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	76.0
1	85.0
2	94.0
3	299.0
4	504.0
5	504.0
6	741.0
7	978.0
8	979.5
9	981.0
10	1379.5
11	1778.0
12	1778.0
13	3109.5
14	4441.0
15	6164.5
16	7888.0
17	7806.5
18	7725.0
19	7725.0
20	9265.5
21	10806.0
22	10527.0
23	10248.0
24	11925.0
25	13602.0
26	13602.0
27	16019.5
28	18437.0
29	21494.5
30	24552.0
31	28420.0
32	32288.0
33	32288.0
34	36865.5
35	41443.0
36	46791.0
37	52139.0
38	57604.5
39	63070.0
40	63070.0
41	66274.5
42	69479.0
43	72271.5
44	75064.0
45	78680.5
46	82297.0
47	82297.0
48	88677.5
49	95058.0
50	95916.5
51	96775.0
52	99383.0
53	101991.0
54	101991.0
55	100418.5
56	98846.0
57	97702.5
58	96559.0
59	98737.5
60	100916.0
61	100916.0
62	93390.5
63	85865.0
64	79634.5
65	73404.0
66	64457.5
67	55511.0
68	55511.0
69	49477.5
70	43444.0
71	36275.5
72	29107.0
73	21440.0
74	13773.0
75	13773.0
76	10252.0
77	6731.0
78	5484.5
79	4238.0
80	3497.0
81	2756.0
82	2756.0
83	2105.5
84	1455.0
85	1209.0
86	963.0
87	838.0
88	713.0
89	713.0
90	559.0
91	405.0
92	222.5
93	40.0
94	26.5
95	13.0
96	13.0
97	8.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1426460.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.07389228801176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.85880245120124	51.90161432555966
2	7.784636112682137	9.197375104439026
3	1.7409013978936996	3.0852546498966458
4	0.6732786518328143	1.5909276223275777
5	0.4062271197775705	1.1998708559104727
6	0.24514302021656936	0.8688931422858904
7	0.17116709120674908	0.7078054416439846
8	0.12951355213359794	0.6120695702862368
9	0.10165284961218697	0.5404526539883812
>10	0.6847902962254919	7.9456825594932265
>50	0.0951663661853599	3.9499575332501258
>100	0.09584625456407395	11.769997155236878
>500	0.010252184595295833	4.022411937835364
>1k	0.002622651873215213	2.607687447846663
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3629	0.2544060120858629	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2984	0.2091891816104202	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2743	0.19229421084362688	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2420	0.16965074379933542	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2186	0.15324649832452364	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1884	0.13207520715617682	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1862	0.13053292766709196	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1667	0.11686272310474882	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1518	0.10641728474685586	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	1484	0.10403376190008833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4020722628044248E-4	0.0
2	7.010361314022124E-5	0.0	0.0	0.0018226939416457525	0.0
3	7.010361314022124E-5	0.0	0.0	0.0026639372993284074	0.0
4	7.010361314022124E-5	0.0	0.0	0.005257770985516594	0.0
5	7.010361314022124E-5	0.0	0.0	0.005888703503778585	0.0
6	7.010361314022124E-5	0.0	0.0	0.005888703503778585	0.0
7	1.4020722628044248E-4	0.0	0.0	0.006800050474601461	0.0
8	1.4020722628044248E-4	0.0	0.0	0.007360879379723231	0.0
9	1.4020722628044248E-4	0.0	0.0	0.0112165781024354	0.0
10	2.1031083942066374E-4	0.0	0.0	0.017736214124475975	0.0
11	2.8041445256088497E-4	0.0	0.0	0.01955890806612173	0.0
12	2.8041445256088497E-4	0.0	0.0	0.02131149839462726	0.0
13	2.8041445256088497E-4	0.0	0.0	0.022573363431151242	0.0
14	2.8041445256088497E-4	0.0	0.0	0.024045539307095888	0.0
15	3.5051806570110625E-4	0.0	0.0	0.025587818796180755	0.0
16	3.5051806570110625E-4	0.0	0.0	0.028251756095509162	0.0
17	4.206216788413275E-4	0.0	0.0	0.03098579700797779	0.0
18	4.206216788413275E-4	0.0	0.0	0.032247662044501774	0.0
19	4.907252919815488E-4	0.0	0.0	0.03491159934383018	7.010361314022124E-5
20	4.907252919815488E-4	0.0	0.0	0.03701470773803682	7.010361314022124E-5
21	5.608289051217699E-4	0.0	0.0	0.04129102813959031	7.010361314022124E-5
22	5.608289051217699E-4	0.0	0.0	0.04696942080394823	7.010361314022124E-5
23	5.608289051217699E-4	7.010361314022124E-5	0.0	0.05475092186251279	7.010361314022124E-5
24	6.309325182619912E-4	7.010361314022124E-5	0.0	0.06610770719122863	7.010361314022124E-5
25	6.309325182619912E-4	7.010361314022124E-5	0.0	0.07101496011104412	7.010361314022124E-5
26	6.309325182619912E-4	7.010361314022124E-5	0.0	0.07739438890680425	7.010361314022124E-5
27	6.309325182619912E-4	7.010361314022124E-5	0.0	0.08882127784866033	7.010361314022124E-5
28	6.309325182619912E-4	7.010361314022124E-5	0.0	0.11553075445508461	7.010361314022124E-5
29	6.309325182619912E-4	7.010361314022124E-5	0.0	0.15976613434656423	7.010361314022124E-5
30	6.309325182619912E-4	7.010361314022124E-5	0.0	0.21395622730395525	7.010361314022124E-5
31	6.309325182619912E-4	7.010361314022124E-5	0.0	0.40372670807453415	7.010361314022124E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACTG	80	6.962546E-7	20.8125	5
GCTTTAT	675	0.0	19.185186	1
ACGCTAA	60	9.238306E-4	18.5	34
TATTGAG	725	0.0	17.351725	5
CGAACTA	75	2.067707E-4	17.266666	24
CAAGACG	385	0.0	16.337664	4
GGACCGT	80	3.3832804E-4	16.1875	6
GGTATCA	2055	0.0	16.114355	1
CGAACGA	115	1.2426317E-6	16.086956	16
AAGACGG	325	0.0	15.938462	5
TAGACTC	105	9.346173E-6	15.857144	5
ACGGACC	340	0.0	15.779411	8
GTAAACG	220	1.8189894E-12	15.136364	27
TTTATTG	920	0.0	15.081522	3
CGCAAGA	360	0.0	14.902779	2
GCGCAAG	385	0.0	14.896105	1
TAGCGCA	75	0.0041050515	14.8	4
TTGCGTT	75	0.0041050515	14.8	4
TATCGCT	75	0.0041050515	14.8	7
GTCTAAA	140	5.9972444E-7	14.535715	1
>>END_MODULE