FastQCFastQC Report
Fri 10 Feb 2017
ERR1630658.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630658.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2010905
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT72550.36078283161064295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69140.34382529259214134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54400.27052496264119885No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33260.16539816649717418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG4100.016.6951225
CAAGACG4550.016.670334
TCTAGCG2650.016.05660428
GTACGAC700.002593400415.8571423
GACGGAC4350.015.7356317
TACGTTA957.063882E-515.57894819
ACGGACC4900.015.102048
GGTATCA35650.014.8934091
CTTATAC13450.014.8550237
TCTTATA21150.014.69503537
CGGACCA4550.014.63736259
TACGACC1651.646913E-814.5757594
CGCAATA2800.014.53571436
TACGACG2450.014.3469385
TACGAGT1304.447662E-614.2307694
CGCAAGA5200.014.2307692
CTAGCGG3250.014.23076929
GCAGTCG3400.014.14705859
TTAACGG1051.6566052E-414.09523835
AGACGGA5050.013.9207936
Produced by FastQC (version 0.11.5)