FastQCFastQC Report
Fri 10 Feb 2017
ERR1630657.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630657.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970314
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT40600.41842125332624286No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA38470.3964695964399153No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC34370.3542152334192849No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT33080.34092056798108655No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG29350.302479403574513No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC28310.2917612236863531No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28120.2898030946683239No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC28050.2890816787143131No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC25990.2678514377819964No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC24920.2568240796278318No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG24530.2528047621697718No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA24400.25146498968375186No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG24260.25002215777573034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23220.2393039778875704No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC23210.23920091846556887No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAAC23030.2373458488695412No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC22380.23064698643944126No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22020.2269368472473859No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG21730.22394812400934133No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC21540.22198999499131208No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC20720.21353912238718603No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAG20710.21343606296518447No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG20490.21116875568115062No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA20410.21034428030513833No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG19880.20488213093905686No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC19540.20137811059100455No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG19490.20086281348099688No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGA19480.20075975405899532No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG19080.19663737717893381No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGG18980.19560678295891842No Hit
GAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATG18370.18932015821682466No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAG18260.18818650457480773No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG18040.1859191972907739No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGT17980.18530084075876468No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCC17920.18468248422675548No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAA17690.1823121175207201No Hit
GAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGG17680.18220905809871857No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG17140.1766438493106355No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGG16930.17447960144860325No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGG16880.17396430433859555No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT16050.1654103723124679No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC15850.1633491838724372No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC15780.16262776791842642No Hit
AGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTA15550.16025740121239104No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT15430.15902068814837259No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTT15230.15695949970834183No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT15200.15665032144233723No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAG15150.15613502433232954No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC14590.15036369670024344No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA14390.14830250826021268No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG14280.14716885461819576No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGC14180.14613826039818037No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT14160.1459321415541773No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG13920.1434587154261404No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTT13620.14036693276609427No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTAT13010.13408030802400048No Hit
GGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGAAACCT12960.13356501091399278No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC12750.1314007630519605No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG12660.13047322825394664No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC12630.13016404998794207No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC12200.12573249484187593No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA12010.12377436582384671No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC11900.1226407121818298No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG11760.12119788027380826No Hit
GTGCCAGGTGGGGAGTTTGACTGGGGCGGTACACCTGTCAAAC11610.1196519889437852No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11550.11903363241177597No Hit
CCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG11440.11789997876975908No Hit
GGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTT11420.117693859925756No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGG11410.11759080050375446No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC11400.11748774108175293No Hit
GGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG11390.11738468165975138No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC11210.11552961206372371No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGG11050.1138806613116991No Hit
TCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA11010.11346842362369294No Hit
CCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGG10770.11099499749565604No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGG10750.11078887865165296No Hit
GTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTTTTGCC10600.1092429873216299No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAA10580.10903686847762682No Hit
GTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGT10340.10656344234958993No Hit
GAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAA10310.10625426408358532No Hit
GGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCC10160.10470837275356225No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTA10140.10450225390955918No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGC9970.10275024373553304No Hit
CCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCA9890.10192576835952073No Hit
ATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGT9830.1013074118275115No Hit
CCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTC9830.1013074118275115No Hit
GCGTAGGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT9810.10110129298350842No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC9740.10037987702949766No Hit
ATACGAATGCCCCCGGCCGTCCCTCTTAATCATGGCCTCAGTT9730.10027681760749613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAG556.26007E-726.909099
CGGTAGC601.3370482E-624.66666610
ATGCAGG901.4188117E-1024.66666636
TGCATCG400.001930971923.1257
CCTTGCT400.001930971923.12515
GGTATCA15800.022.3639241
ATCTTAC603.7255166E-521.5833321
TAGGGGT603.7255166E-521.5833324
GCCGTCT603.7255166E-521.58333236
AGTACCG450.003825385120.5555555
GCAGTCG1900.020.4473699
AATCCAT555.142301E-420.18181823
AAGACGG7700.018.980525
TTGGCGC609.235402E-418.510
GGAGCGT500.00703411118.58
TTCACTA609.235402E-418.530
GGATAAT609.235402E-418.51
AGCGGCC801.616248E-518.514
TAGATGC902.1515134E-618.537
ATCTGAC609.235402E-418.57
Produced by FastQC (version 0.11.5)