FastQCFastQC Report
Fri 10 Feb 2017
ERR1630656.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630656.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences537102
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98841.8402463591645535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81041.5088381722652306No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64681.204240535317314No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48450.9020632952400102No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15110.2813245901150992No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14700.273691030753935No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA12410.23105480895621316No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT12390.23068244020688805No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT12080.22491072459234931RNA PCR Primer, Index 47 (95% over 22bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11400.21225018711529656No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC11100.20666465587542032No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC9720.18097121217198966No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9330.17371002156015058No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC8850.16477317157634863No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA8390.15620869034187176No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8190.15248500284862093No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG8080.15043697472733297No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8000.14894749973003266No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC7750.14429289036346915No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC7740.1441067059888066No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7630.14205867786751866No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC7220.13442511850635447No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC6800.12660537477052775No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC6730.12530208414788996No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC6710.1249297153985649No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.12455734664923981No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC6640.12362642477592711No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC6590.1226955029026144No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT6290.11710997166273816No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG6130.11413102166813753No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG6110.11375865291881244No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG5580.10389088106169776No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG5540.10314614356304762No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG5520.10277377481372253No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5400.10053956231777204No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5380.10016719356844696No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTAT250.0054937729.5999986
AATCGGA509.079322E-625.89999817
CTCTGAA400.001929854623.1250022
TGATGCA400.001929854623.1250025
AATACCC502.6995066E-422.1999995
ATCGGAG603.7215308E-521.58333218
GCATAAA450.003823194420.55555511
CACACCG450.003823194420.5555555
GCCGTCT1850.020.00000236
TAATACC656.8933834E-519.9230774
GCAGTCG3800.019.4736849
CAGTCGG3900.019.44871910
ATGCTAG902.1478754E-618.515
TAGTTTA609.228086E-418.57
GTAATAC801.6140582E-518.53
GTACTGC801.6140582E-518.56
TTTGGCA701.2176634E-418.527
TATTAGA1306.9303496E-1018.52
TAGGGGT609.228086E-418.54
TATACAC3200.018.53
Produced by FastQC (version 0.11.5)