FastQCFastQC Report
Fri 10 Feb 2017
ERR1630655.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630655.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences967764
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54730.5655304392393187No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48750.5037385147618635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40970.4233470143547394No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA28830.297903207806862No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24950.257810788580687No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC24060.24861433159323967No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC20970.21668505958064155No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC18310.18919901959568655No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC15770.16295295133937612No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG15160.15664976171876616No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC15090.1559264448770568No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14900.15396315630670288No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT14800.1529298465328324No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC14420.14900326939212452No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA13770.14228675586196635No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC13730.14187343195241814No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG13030.1346402635353247No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG12650.13071368639461686No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC12170.12575379948003854No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT11730.12120723647500836No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT11040.114077399035302No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC10910.11273409632927035No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT10770.11128746264585168No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG10290.10632757573127331No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC10060.10395096325137121No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC9830.10157435077146908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACG601.3370427E-624.6666668
GGTATCA21550.021.4617181
TAGAACT1001.2891178E-820.3499984
GCCGTCT1550.020.29032336
CTGACGC656.9006885E-519.9230779
TACGTAC656.9006885E-519.92307710
TAAGGTA759.263293E-619.7333325
CGGACCT851.2448927E-619.58823635
TGTACGT609.23538E-418.512
TACCGTT609.23538E-418.518
TTAGAAC1002.8737486E-718.4999983
GCGTTAC650.001579689717.07692314
AGAGACG650.001579689717.0769238
GAGCCGC3350.016.56716316
CTAGACT904.4447897E-516.4444454
TACCCTT803.3818738E-416.18755
GGCACCG1504.665708E-916.0333339
TCTAGAC700.002592096815.8571423
CTTATAC6300.015.85714237
CTACGTA700.002592096815.8571429
Produced by FastQC (version 0.11.5)