##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630655.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	967764
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88375058382002	33.0	31.0	34.0	30.0	34.0
2	32.04135409046007	33.0	31.0	34.0	30.0	34.0
3	32.14291500820448	34.0	31.0	34.0	30.0	34.0
4	35.75380671320694	37.0	35.0	37.0	33.0	37.0
5	35.741676689771474	37.0	35.0	37.0	35.0	37.0
6	35.78188690631187	37.0	35.0	37.0	35.0	37.0
7	35.76451593570333	37.0	35.0	37.0	35.0	37.0
8	35.76010163634936	37.0	35.0	37.0	35.0	37.0
9	37.46200726623433	39.0	37.0	39.0	35.0	39.0
10	37.36132776172703	39.0	37.0	39.0	34.0	39.0
11	37.477091522313295	39.0	37.0	39.0	35.0	39.0
12	37.399329795280664	39.0	37.0	39.0	34.0	39.0
13	37.44911776011507	39.0	37.0	39.0	35.0	39.0
14	38.669746963102575	40.0	38.0	41.0	35.0	41.0
15	38.62643268400147	40.0	38.0	41.0	34.0	41.0
16	38.59166387673028	40.0	38.0	41.0	34.0	41.0
17	38.58597137318603	40.0	38.0	41.0	34.0	41.0
18	38.58254078473677	40.0	38.0	41.0	34.0	41.0
19	38.61109010047904	40.0	38.0	41.0	34.0	41.0
20	38.60471871241336	40.0	38.0	41.0	34.0	41.0
21	38.522504453565126	40.0	38.0	41.0	34.0	41.0
22	38.433285387759824	40.0	38.0	41.0	34.0	41.0
23	38.38362452002761	40.0	38.0	41.0	34.0	41.0
24	38.322006191592166	40.0	38.0	41.0	34.0	41.0
25	38.27566328154385	40.0	38.0	41.0	34.0	41.0
26	38.07872477174187	40.0	37.0	41.0	33.0	41.0
27	37.93628095279428	40.0	37.0	41.0	33.0	41.0
28	37.78646446861011	40.0	37.0	41.0	33.0	41.0
29	37.666358740354056	40.0	37.0	41.0	32.0	41.0
30	37.57269954245043	40.0	37.0	41.0	32.0	41.0
31	37.527326910279776	40.0	37.0	41.0	32.0	41.0
32	37.39744813818245	40.0	36.0	41.0	32.0	41.0
33	37.31501998421103	39.0	36.0	41.0	32.0	41.0
34	37.230272049797264	39.0	36.0	41.0	31.0	41.0
35	37.14671138831368	39.0	36.0	41.0	31.0	41.0
36	37.091416915694325	39.0	36.0	41.0	31.0	41.0
37	36.87701133747484	39.0	35.0	41.0	31.0	41.0
38	36.737958841205085	39.0	35.0	41.0	30.0	41.0
39	36.63217168648555	39.0	35.0	41.0	30.0	41.0
40	36.48160811933488	39.0	35.0	41.0	30.0	41.0
41	36.33693648451482	39.0	35.0	41.0	30.0	41.0
42	36.1882969401631	39.0	35.0	40.0	30.0	41.0
43	35.28770650695831	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	1.0
13	3.0
14	0.0
15	4.0
16	10.0
17	15.0
18	42.0
19	103.0
20	197.0
21	385.0
22	646.0
23	1165.0
24	1823.0
25	2919.0
26	4176.0
27	6359.0
28	8982.0
29	12234.0
30	16108.0
31	20841.0
32	26788.0
33	34328.0
34	45709.0
35	60366.0
36	85496.0
37	131616.0
38	225190.0
39	282252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.08595484022964	19.776722424062065	12.455516014234876	23.681806721473418
2	20.209472557359025	20.900963458033157	33.129254652993914	25.760309331613907
3	21.786716596194942	23.40022980809371	28.665459760850787	26.147593834860565
4	14.0047573581989	15.396005637738124	34.35021348179928	36.24902352226369
5	14.204186144555905	37.61660900798128	33.398535180064556	14.78066966739825
6	34.46821745797529	34.390409232002845	15.657019686617812	15.484353623404054
7	28.688192575875938	30.968603915830716	20.692544876643478	19.650658631649865
8	29.086946817612557	30.402866814636624	19.7596728127932	20.750513554957614
9	26.94365568465039	12.919162109770562	20.737390520829457	39.39979168474959
10	19.174716149805118	25.57627686088757	31.09942093320272	24.14958605610459
11	36.308748827193405	21.498319838307687	20.20916256442686	21.983768770072043
12	22.545889287057587	26.424934178167405	26.697004641627505	24.332171893147503
13	30.07468763045536	19.169239608003604	25.966971286388002	24.789101475153032
14	23.334821299407707	21.55349858023237	24.548236966863822	30.5634431534961
15	26.998834426575076	26.593363671308296	23.040121351899842	23.36768055021679
16	26.49147932760466	25.16005968397254	24.163639069029227	24.18482191939357
17	24.228220929896132	25.666484804146467	24.735782690821313	25.369511575136087
18	24.082110927870843	22.627934083102904	27.53987542417366	25.75007956485259
19	24.02228229196374	24.79137475665555	26.794755746235655	24.391587205145058
20	25.05125216478398	22.924907312113284	27.120971641846563	24.902868881256175
21	26.386495054579424	24.19432836931318	26.096445001053976	23.32273157505342
22	26.652262328418914	23.39134334403842	26.266941113742607	23.689453213800057
23	24.733509409318803	23.162981884013046	27.083979151942	25.019529554726148
24	23.67684683455884	24.983983698505007	26.51245551601423	24.82671395092192
25	24.67574739295944	24.144832831144782	26.66176877833852	24.517650997557254
26	24.335478484423888	25.594669774862467	26.212795681591793	23.857056059121852
27	25.56522044630716	23.719935852129236	26.55885112486102	24.155992576702584
28	23.697203037104085	24.016185764297905	27.56715480220384	24.71945639639416
29	23.20906749992767	24.699203524826302	27.846561765058425	24.245167210187606
30	22.55983896900484	25.924192261749766	27.76648025758346	23.749488511661934
31	24.51641102582861	25.586299965694113	25.543934264965422	24.353354743511847
32	23.78782430427253	24.461025621949155	26.39476153277039	25.35638854100793
33	22.667923171351692	24.239897330340867	27.187413460306438	25.904766038000997
34	24.396547092059635	24.184305264506637	27.230915801786388	24.188231841647344
35	23.727375682501105	24.12530327641863	28.161204591201987	23.986116449878274
36	22.76081772002265	25.902182763566323	26.659702158790783	24.677297357620244
37	24.39809705672044	24.23814070372529	27.453284065123317	23.910478174430956
38	23.053967702869706	23.59480203851352	27.69363191852559	25.65759834009118
39	23.794230824870528	24.137393000772917	27.630496691342106	24.437879483014456
40	22.83407938299007	23.97970992928028	28.26877213866191	24.917438549067747
41	22.225563257157738	24.450072538346127	28.679409442798036	24.644954761698102
42	23.168975080701493	24.390243902439025	28.95168656821291	23.489094448646572
43	22.054653820559558	22.81341318751266	28.466754291335487	26.665178700592296
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	82.0
1	122.5
2	163.0
3	435.0
4	707.0
5	707.0
6	1072.0
7	1437.0
8	1398.0
9	1359.0
10	1886.0
11	2413.0
12	2413.0
13	4240.0
14	6067.0
15	8425.0
16	10783.0
17	10192.0
18	9601.0
19	9601.0
20	10631.0
21	11661.0
22	9782.0
23	7903.0
24	8644.5
25	9386.0
26	9386.0
27	10651.5
28	11917.0
29	14026.0
30	16135.0
31	18974.5
32	21814.0
33	21814.0
34	26459.0
35	31104.0
36	35262.5
37	39421.0
38	43241.5
39	47062.0
40	47062.0
41	50771.0
42	54480.0
43	55923.0
44	57366.0
45	58364.5
46	59363.0
47	59363.0
48	63668.0
49	67973.0
50	66860.5
51	65748.0
52	69267.5
53	72787.0
54	72787.0
55	70800.0
56	68813.0
57	64437.5
58	60062.0
59	58477.5
60	56893.0
61	56893.0
62	54426.0
63	51959.0
64	45717.5
65	39476.0
66	35726.5
67	31977.0
68	31977.0
69	26433.0
70	20889.0
71	17615.0
72	14341.0
73	10314.5
74	6288.0
75	6288.0
76	4705.5
77	3123.0
78	2687.5
79	2252.0
80	1975.0
81	1698.0
82	1698.0
83	1364.5
84	1031.0
85	981.0
86	931.0
87	828.5
88	726.0
89	726.0
90	609.5
91	493.0
92	276.0
93	59.0
94	38.5
95	18.0
96	18.0
97	10.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	967764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.382995224020306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.85172518011797	40.67911884183433
2	8.335491537741529	7.89921111445337
3	1.9270433012599084	2.7392725062023566
4	0.8148441901080766	1.5443903347284675
5	0.47806930329583996	1.132617775740875
6	0.3189439767913314	0.9067512557420219
7	0.248587584076466	0.8245177016332154
8	0.21313398593846294	0.8079141310238881
9	0.16293026891020063	0.6948111738258341
>10	1.290940369244336	12.59517478604917
>50	0.18494590911909478	6.179509759485303
>100	0.15364616687761604	14.357995557058587
>500	0.01422649693051964	4.64150041235218
>1k	0.005252860405114945	4.4296274714630135
>5k	2.1886918354645604E-4	0.5675871784074421
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5473	0.5655304392393187	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4875	0.5037385147618635	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4097	0.4233470143547394	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2883	0.297903207806862	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2495	0.257810788580687	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2406	0.24861433159323967	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2097	0.21668505958064155	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1831	0.18919901959568655	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1577	0.16295295133937612	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1516	0.15664976171876616	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1509	0.1559264448770568	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1490	0.15396315630670288	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1480	0.1529298465328324	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1442	0.14900326939212452	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1377	0.14228675586196635	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1373	0.14187343195241814	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1303	0.1346402635353247	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1265	0.13071368639461686	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	1217	0.12575379948003854	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	1173	0.12120723647500836	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	1104	0.114077399035302	No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC	1091	0.11273409632927035	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	1077	0.11128746264585168	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	1029	0.10632757573127331	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	1006	0.10395096325137121	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC	983	0.10157435077146908	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.099929321611467E-4	0.0
2	0.0	0.0	0.0	0.001963288570353929	0.0
3	0.0	0.0	0.0	0.0027899363894503203	0.0
4	0.0	0.0	0.0	0.005166548869352445	0.0
5	0.0	1.0333097738704891E-4	0.0	0.005579872778900641	0.0
6	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.005786534733674739	0.0
7	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.006509851575384081	0.0
8	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.007336499394480472	0.0
9	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.011573069467349479	0.0
10	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.01715294224625012	0.0
11	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.0188062378844429	0.0
12	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.01994287863570044	0.0
13	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.020769526454796832	0.0
14	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.022112829160828466	0.0
15	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.02459277261811764	0.0
16	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.027382709007567962	0.0
17	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.03120595517088877	0.0
18	0.0	1.0333097738704891E-4	1.0333097738704891E-4	0.0329625817864686	0.0
19	1.0333097738704891E-4	1.0333097738704891E-4	1.0333097738704891E-4	0.0376124757688858	0.0
20	1.0333097738704891E-4	1.0333097738704891E-4	1.0333097738704891E-4	0.04040241215833612	0.0
21	1.0333097738704891E-4	1.0333097738704891E-4	1.0333097738704891E-4	0.04515563711814037	0.0
22	1.0333097738704891E-4	1.0333097738704891E-4	1.0333097738704891E-4	0.05424876312820068	0.0
23	1.0333097738704891E-4	1.0333097738704891E-4	1.0333097738704891E-4	0.06406520597997033	0.0
24	1.0333097738704891E-4	2.0666195477409783E-4	1.0333097738704891E-4	0.08204479604531684	0.0
25	1.0333097738704891E-4	2.0666195477409783E-4	1.0333097738704891E-4	0.08803799273376567	0.0
26	1.0333097738704891E-4	2.0666195477409783E-4	1.0333097738704891E-4	0.09878441438201875	0.0
27	1.0333097738704891E-4	3.099929321611467E-4	1.0333097738704891E-4	0.114077399035302	0.0
28	1.0333097738704891E-4	4.1332390954819566E-4	1.0333097738704891E-4	0.14827995255041518	0.0
29	1.0333097738704891E-4	5.166548869352445E-4	1.0333097738704891E-4	0.19849880756052096	0.0
30	1.0333097738704891E-4	5.166548869352445E-4	1.0333097738704891E-4	0.2832302090179011	0.0
31	1.0333097738704891E-4	5.166548869352445E-4	1.0333097738704891E-4	0.5525107360885505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGACG	60	1.3370427E-6	24.666666	8
GGTATCA	2155	0.0	21.461718	1
TAGAACT	100	1.2891178E-8	20.349998	4
GCCGTCT	155	0.0	20.290323	36
CTGACGC	65	6.9006885E-5	19.923077	9
TACGTAC	65	6.9006885E-5	19.923077	10
TAAGGTA	75	9.263293E-6	19.733332	5
CGGACCT	85	1.2448927E-6	19.588236	35
TGTACGT	60	9.23538E-4	18.5	12
TACCGTT	60	9.23538E-4	18.5	18
TTAGAAC	100	2.8737486E-7	18.499998	3
GCGTTAC	65	0.0015796897	17.076923	14
AGAGACG	65	0.0015796897	17.076923	8
GAGCCGC	335	0.0	16.567163	16
CTAGACT	90	4.4447897E-5	16.444445	4
TACCCTT	80	3.3818738E-4	16.1875	5
GGCACCG	150	4.665708E-9	16.033333	9
TCTAGAC	70	0.0025920968	15.857142	3
CTTATAC	630	0.0	15.857142	37
CTACGTA	70	0.0025920968	15.857142	9
>>END_MODULE