FastQCFastQC Report
Fri 10 Feb 2017
ERR1630654.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630654.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138806
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT84330.7405124314413518No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67730.594745724908369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67380.5916723304935169No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55100.4838400921667079No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16900.14840104460285597No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.13742463597838436No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14200.12469200197399732No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13730.12056487233119602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAA502.702081E-422.214
GGTTAAC500.007034843318.51
TTGACGG1653.6379788E-1217.93939233
ATTGACG1800.017.47222132
CGAATTA1005.877695E-616.65000215
ATGCCGT1005.877695E-616.65000234
GGTATCA43150.016.5921211
TCGCACG1504.669346E-916.03333322
CGATCGC1504.669346E-916.03333319
GCCGTCT1101.4517658E-515.13636436
TGCCGTC1101.4517658E-515.13636435
AAGACGG1601.096123E-815.031255
ACCCTTA750.00410437514.86
TAGGGGT750.00410437514.84
GCGTATA750.00410437514.89
CGTGGTC1405.9941704E-714.53571432
GTAGCAC908.2739315E-414.3888883
TCGGCGC1551.2098644E-714.3225818
TCGTTCT1702.4358087E-814.14705930
GGCCGAT1854.8949005E-914.016
Produced by FastQC (version 0.11.5)