FastQCFastQC Report
Fri 10 Feb 2017
ERR1630653.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630653.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1178469
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22340.18956799033322047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22270.18897399931606176No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT15700.13322369956273775No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14050.11922248272971118No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC502.7021585E-422.23
GCCGTCT2200.021.86363636
ATAGTAT555.1432E-420.1818188
GAACCGT759.266098E-619.7333346
GTGTAGA1351.1514203E-917.8148141
TAATAGT1258.578354E-917.764
GGTATCA13650.017.4835171
ACTGCGT852.7232098E-517.4117648
TAGACCA852.7232098E-517.4117645
TTCTATA752.0673223E-417.2666662
TAATACT1107.808012E-716.8181824
TATACTG1554.0017767E-1016.7096775
GAGCGAA1005.8780442E-616.65000216
ATGCCGT2800.016.51785934
TCTTATA11500.016.40869537
CCGTCTT2850.016.22807137
GTACGGG3350.016.0149251
CGCGGTT957.0599155E-515.57894810
GTGCTAT957.0599155E-515.5789481
GTATAGT957.0599155E-515.5789481
Produced by FastQC (version 0.11.5)