##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630653.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1178469
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03509808064531	33.0	31.0	34.0	30.0	34.0
2	32.20551410346815	33.0	31.0	34.0	30.0	34.0
3	32.31165435832423	34.0	31.0	34.0	30.0	34.0
4	35.89384531964778	37.0	35.0	37.0	35.0	37.0
5	35.866836548097574	37.0	35.0	37.0	35.0	37.0
6	35.90222059298972	37.0	35.0	37.0	35.0	37.0
7	35.879097371250324	37.0	35.0	37.0	35.0	37.0
8	35.85947954507076	37.0	35.0	37.0	35.0	37.0
9	37.62115337781478	39.0	37.0	39.0	35.0	39.0
10	37.510430906540606	39.0	37.0	39.0	35.0	39.0
11	37.630197315330314	39.0	37.0	39.0	35.0	39.0
12	37.54529308789625	39.0	37.0	39.0	35.0	39.0
13	37.59703903963533	39.0	37.0	39.0	35.0	39.0
14	38.88658844653529	40.0	38.0	41.0	36.0	41.0
15	38.87410020967883	40.0	38.0	41.0	35.0	41.0
16	38.848169107545466	40.0	38.0	41.0	35.0	41.0
17	38.82312390058627	40.0	38.0	41.0	35.0	41.0
18	38.807264340428134	40.0	38.0	41.0	35.0	41.0
19	38.83613145530345	40.0	38.0	41.0	35.0	41.0
20	38.81625651586932	40.0	38.0	41.0	35.0	41.0
21	38.742947841648785	40.0	38.0	41.0	35.0	41.0
22	38.68624291347503	40.0	38.0	41.0	34.0	41.0
23	38.67642509051999	40.0	38.0	41.0	34.0	41.0
24	38.62928935763266	40.0	38.0	41.0	34.0	41.0
25	38.58398820842975	40.0	38.0	41.0	34.0	41.0
26	38.400968544781406	40.0	38.0	41.0	34.0	41.0
27	38.2997660523951	40.0	38.0	41.0	34.0	41.0
28	38.22555790606287	40.0	38.0	41.0	34.0	41.0
29	38.131938981848485	40.0	38.0	41.0	33.0	41.0
30	38.06382263767651	40.0	38.0	41.0	33.0	41.0
31	38.01963649446867	40.0	38.0	41.0	33.0	41.0
32	37.92423390008562	40.0	37.0	41.0	33.0	41.0
33	37.91445680794319	40.0	37.0	41.0	33.0	41.0
34	37.84130341994571	40.0	37.0	41.0	33.0	41.0
35	37.795529623604864	40.0	37.0	41.0	33.0	41.0
36	37.766005724376285	40.0	37.0	41.0	33.0	41.0
37	37.584755305400485	40.0	37.0	41.0	32.0	41.0
38	37.501107793247	40.0	37.0	41.0	32.0	41.0
39	37.44756459440172	40.0	37.0	41.0	32.0	41.0
40	37.37204118224578	40.0	36.0	41.0	32.0	41.0
41	37.26021643335548	40.0	36.0	41.0	31.0	41.0
42	37.204201383320225	40.0	36.0	41.0	31.0	41.0
43	36.327995051206265	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	2.0
12	0.0
13	2.0
14	1.0
15	5.0
16	10.0
17	13.0
18	34.0
19	74.0
20	141.0
21	295.0
22	526.0
23	885.0
24	1471.0
25	2452.0
26	3725.0
27	5349.0
28	7993.0
29	11383.0
30	15511.0
31	20944.0
32	27207.0
33	35754.0
34	48329.0
35	65475.0
36	92505.0
37	142393.0
38	266610.0
39	429374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.693045807738685	18.048417056367203	11.920381444060048	26.338155691834064
2	19.328637410063397	19.905572399443685	34.425513102169006	26.340277088323923
3	20.60766978172527	21.52504647979709	29.097328822395834	28.769954916081797
4	15.092802610845087	14.920714927588252	32.87621481769991	37.11026764386675
5	15.051392951363166	35.95105174595174	34.04527399532783	14.952281307357257
6	36.81938175717817	33.22794235571746	14.87888098880836	15.073794898296008
7	30.510263740497205	29.414095746260614	20.047705964263805	20.027934548978376
8	27.62185513577362	33.16073651491893	19.321424661997895	19.895983687309553
9	26.856879561532804	13.117697622932806	18.703674004152845	41.321748811381546
10	16.89463193346622	26.328312412121154	33.02657940090066	23.75047625351197
11	36.48963188679549	20.59417770004981	20.82693732291643	22.089253090238266
12	21.964090697336967	24.446464013902784	28.35526433024543	25.23418095851482
13	31.104763892813477	19.215185125786082	24.271151807981372	25.40889917341907
14	23.831513599424337	20.115845219517865	24.28184364629023	31.77079753476757
15	26.826755731376895	26.474943337499756	21.205309600846522	25.492991330276826
16	26.363951873150672	25.56342169373993	22.872048394993843	25.200578038115555
17	24.310100647535066	25.92872616929253	24.66590126681313	25.095271916359273
18	24.334539135098165	24.635438013218845	25.820450092450457	25.20957275923253
19	25.26413507695154	24.652918320295232	25.645392454107828	24.437554148645404
20	25.774118793112077	24.202079138271777	25.35594911703235	24.667852951583793
21	25.80678829905581	24.27692200643377	24.54073887391183	25.37555082059859
22	25.837930399526844	24.56305596498508	24.516385242208322	25.082628393279755
23	25.36231330650191	24.626188724523086	24.767304019028078	25.244193949946926
24	25.310805799728293	24.758054730332322	24.86607623959561	25.065063230343775
25	25.721847583602113	24.547103063381385	24.587918731846152	25.14313062117035
26	25.278984852380503	24.896030358032327	24.882029141199304	24.942955648387866
27	25.639282832217052	24.426607742757763	24.426692598617358	25.507416826407827
28	25.00693696652182	24.49915950271072	25.056747356103553	25.437156174663905
29	24.56534707319412	24.774516767093576	25.32344932280781	25.336686836904494
30	24.936676314777902	25.533297863584025	25.132778206299868	24.397247615338205
31	25.17877008219987	25.034430265030306	24.59538604749043	25.191413605279394
32	24.577736028694858	24.835528130141736	25.225525660836222	25.361210180327188
33	24.662591888288958	24.67752651957752	25.417893894536046	25.241987697597473
34	25.144403459064264	24.181374308530813	25.3107209438687	25.363501288536227
35	24.946010459333255	24.24255538329816	26.128901142075012	24.682533015293572
36	24.596489173665155	24.564583370457772	25.392437136657815	25.446490319219254
37	24.915632061598565	23.79197076885349	25.792532514644	25.49986465490395
38	24.407854597787466	23.91993340512139	26.40587066779016	25.266341329300985
39	24.488467664401863	23.76846569574592	26.33561001604624	25.407456623805974
40	23.68810719671031	23.520262306433175	27.427450361443533	25.36418013541298
41	23.32169959498298	23.443892032798487	27.588506782953136	25.645901589265396
42	23.542155118208456	23.35369025404996	27.511542518301287	25.5926121094403
43	22.997719923052706	23.21113240993187	27.939046338936365	25.85210132807906
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	33.5
2	44.0
3	140.0
4	236.0
5	236.0
6	369.5
7	503.0
8	495.5
9	488.0
10	731.5
11	975.0
12	975.0
13	1690.0
14	2405.0
15	3696.0
16	4987.0
17	4908.0
18	4829.0
19	4829.0
20	6092.5
21	7356.0
22	7585.5
23	7815.0
24	9455.0
25	11095.0
26	11095.0
27	13216.5
28	15338.0
29	18770.5
30	22203.0
31	26781.0
32	31359.0
33	31359.0
34	34857.5
35	38356.0
36	42379.5
37	46403.0
38	51293.5
39	56184.0
40	56184.0
41	59413.0
42	62642.0
43	63833.5
44	65025.0
45	69172.5
46	73320.0
47	73320.0
48	75736.0
49	78152.0
50	83501.5
51	88851.0
52	92481.0
53	96111.0
54	96111.0
55	93292.0
56	90473.0
57	86539.5
58	82606.0
59	75530.5
60	68455.0
61	68455.0
62	64465.5
63	60476.0
64	54112.0
65	47748.0
66	42106.0
67	36464.0
68	36464.0
69	31591.0
70	26718.0
71	23459.0
72	20200.0
73	16813.0
74	13426.0
75	13426.0
76	10597.0
77	7768.0
78	6199.0
79	4630.0
80	3563.0
81	2496.0
82	2496.0
83	1864.5
84	1233.0
85	921.5
86	610.0
87	455.0
88	300.0
89	300.0
90	218.0
91	136.0
92	79.5
93	23.0
94	13.5
95	4.0
96	4.0
97	3.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1178469.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.86074608775738
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.08161531321404	55.18457047566626
2	9.080954924347044	11.779950231648872
3	2.4475953143566826	4.762585746302205
4	1.0296839146157326	2.671442677461563
5	0.5717264589275598	1.8541302342076553
6	0.3691557197156737	1.4366229241992985
7	0.23182689656218253	1.052552583196276
8	0.18074484919071807	0.9378596616023332
9	0.14462660652958215	0.84425306432843
>10	0.7424786738811274	8.916382449687694
>50	0.06861432199176228	3.100607328681917
>100	0.04756980913375278	5.560029559555839
>500	0.0027519672391842745	1.1796796306411244
>1k	6.552302950438749E-4	0.7193334328205083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2234	0.18956799033322047	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2227	0.18897399931606176	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	1570	0.13322369956273775	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1405	0.11922248272971118	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.5456757878230147E-4	0.0
3	0.0	0.0	0.0	3.394234383764019E-4	0.0
4	0.0	0.0	0.0	7.637027363469043E-4	0.0
5	0.0	0.0	0.0	8.485585959410048E-4	0.0
6	0.0	0.0	0.0	8.485585959410048E-4	0.0
7	0.0	0.0	0.0	9.334144555351053E-4	0.0
8	0.0	0.0	0.0	0.0010182703151292059	0.0
9	0.0	0.0	0.0	0.0012728378939115073	0.0
10	0.0	0.0	0.0	0.0016971171918820096	0.0
11	0.0	0.0	0.0	0.002121396489852512	0.0
12	0.0	0.0	0.0	0.002460819928228914	0.0
13	0.0	0.0	0.0	0.002460819928228914	0.0
14	0.0	0.0	0.0	0.0025456757878230146	0.0
15	0.0	0.0	0.0	0.002800243366605316	0.0
16	0.0	0.0	0.0	0.0040730812605168235	0.0
17	0.0	0.0	0.0	0.0044973605584873256	0.0
18	0.0	0.0	0.0	0.004751928137269627	0.0
19	0.0	0.0	0.0	0.005006495716051928	0.0
20	0.0	0.0	0.0	0.005176207435240129	0.0
21	0.0	0.0	0.0	0.006449045329151637	0.0
22	0.0	0.0	0.0	0.00890986525738055	0.0
23	0.0	0.0	0.0	0.012728378939115072	0.0
24	0.0	0.0	0.0	0.018583433251108006	0.0
25	0.0	0.0	0.0	0.020450262162178216	0.0
26	0.0	0.0	0.0	0.02469305514188324	0.0
27	0.0	0.0	0.0	0.034451478995204794	0.0
28	0.0	0.0	0.0	0.06084165132897004	0.0
29	0.0	0.0	0.0	0.10360900456439669	0.0
30	0.0	0.0	0.0	0.16674176410240746	0.0
31	0.0	0.0	0.0	0.402810765493195	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGAC	50	2.7021585E-4	22.2	3
GCCGTCT	220	0.0	21.863636	36
ATAGTAT	55	5.1432E-4	20.181818	8
GAACCGT	75	9.266098E-6	19.733334	6
GTGTAGA	135	1.1514203E-9	17.814814	1
TAATAGT	125	8.578354E-9	17.76	4
GGTATCA	1365	0.0	17.483517	1
ACTGCGT	85	2.7232098E-5	17.411764	8
TAGACCA	85	2.7232098E-5	17.411764	5
TTCTATA	75	2.0673223E-4	17.266666	2
TAATACT	110	7.808012E-7	16.818182	4
TATACTG	155	4.0017767E-10	16.709677	5
GAGCGAA	100	5.8780442E-6	16.650002	16
ATGCCGT	280	0.0	16.517859	34
TCTTATA	1150	0.0	16.408695	37
CCGTCTT	285	0.0	16.228071	37
GTACGGG	335	0.0	16.014925	1
CGCGGTT	95	7.0599155E-5	15.578948	10
GTGCTAT	95	7.0599155E-5	15.578948	1
GTATAGT	95	7.0599155E-5	15.578948	1
>>END_MODULE