FastQCFastQC Report
Fri 10 Feb 2017
ERR1630652.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630652.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1832327
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74350.4057681843906683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64170.35021041549898024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55370.30218405339221655No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33560.18315508094352154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38100.015.635171
GCAGTCG3100.015.51612959
CAGTCGG3100.015.516129510
AGTCGGT3000.015.41666711
GCGCAAG3700.015.01
AAGACGG3350.014.9104485
AGGACCG4800.014.6458335
ATACCGT2800.014.5357156
GCTATAC908.2773226E-414.3888883
CGCGGTT3750.014.30666610
CGCAAGA3500.014.2714292
GTATTGG1304.4471453E-614.2307681
TAGGACC5850.014.2307684
CGGTGAT3000.014.18333314
TAACGCC2650.013.9622634
CGTTGGA800.006300504313.8750012
TCGGTGA3200.013.87500113
TACCGTC2800.013.8750017
CAAGACG3600.013.8754
GTCGCCA2405.456968E-1213.87499912
Produced by FastQC (version 0.11.5)