##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630652.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1832327
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.1147071456132	33.0	31.0	34.0	30.0	34.0
2	32.28138590982941	34.0	31.0	34.0	30.0	34.0
3	32.38005879954834	34.0	31.0	34.0	30.0	34.0
4	35.94485100094033	37.0	35.0	37.0	35.0	37.0
5	35.91008428080796	37.0	35.0	37.0	35.0	37.0
6	35.9842036928998	37.0	35.0	37.0	35.0	37.0
7	35.9322069696075	37.0	35.0	37.0	35.0	37.0
8	35.93236578405492	37.0	35.0	37.0	35.0	37.0
9	37.64890655434319	39.0	37.0	39.0	35.0	39.0
10	37.55937231727743	39.0	37.0	39.0	35.0	39.0
11	37.655998629065664	39.0	37.0	39.0	35.0	39.0
12	37.60340212200115	39.0	37.0	39.0	35.0	39.0
13	37.638357673057264	39.0	37.0	39.0	35.0	39.0
14	38.946855010050065	40.0	38.0	41.0	36.0	41.0
15	38.95581902138647	40.0	38.0	41.0	36.0	41.0
16	38.91820401052869	40.0	38.0	41.0	35.0	41.0
17	38.903277635487555	40.0	38.0	41.0	35.0	41.0
18	38.89335964595839	40.0	38.0	41.0	35.0	41.0
19	38.956775182595685	40.0	38.0	41.0	35.0	41.0
20	38.924887315419134	40.0	38.0	41.0	35.0	41.0
21	38.885721271366954	40.0	38.0	41.0	35.0	41.0
22	38.81276376978563	40.0	38.0	41.0	35.0	41.0
23	38.75983762723575	40.0	38.0	41.0	35.0	41.0
24	38.71354185142717	40.0	38.0	41.0	35.0	41.0
25	38.66095898821553	40.0	38.0	41.0	34.0	41.0
26	38.53434512507866	40.0	38.0	41.0	34.0	41.0
27	38.40702942215009	40.0	38.0	41.0	34.0	41.0
28	38.316402585346395	40.0	38.0	41.0	34.0	41.0
29	38.24033373955631	40.0	38.0	41.0	34.0	41.0
30	38.15667290827456	40.0	38.0	41.0	33.0	41.0
31	38.11322487743727	40.0	38.0	41.0	33.0	41.0
32	38.00709425773893	40.0	37.0	41.0	33.0	41.0
33	37.92357423101881	40.0	37.0	41.0	33.0	41.0
34	37.867484897619256	40.0	37.0	41.0	33.0	41.0
35	37.800325487754094	40.0	37.0	41.0	33.0	41.0
36	37.76778489865619	40.0	37.0	41.0	33.0	41.0
37	37.636231960779924	40.0	37.0	41.0	33.0	41.0
38	37.485245264627984	40.0	37.0	41.0	32.0	41.0
39	37.420019461591735	40.0	36.0	41.0	32.0	41.0
40	37.243999024191645	40.0	36.0	41.0	31.0	41.0
41	37.18146488044983	40.0	36.0	41.0	31.0	41.0
42	37.040067084095796	40.0	36.0	41.0	31.0	41.0
43	36.23335299867327	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	6.0
11	2.0
12	6.0
13	3.0
14	5.0
15	7.0
16	4.0
17	18.0
18	52.0
19	95.0
20	252.0
21	388.0
22	836.0
23	1429.0
24	2286.0
25	3760.0
26	5821.0
27	8690.0
28	12576.0
29	17800.0
30	24023.0
31	31608.0
32	41437.0
33	53970.0
34	72337.0
35	96778.0
36	137660.0
37	217601.0
38	403218.0
39	699655.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.013050072394286	18.780217723146578	11.991964316412956	25.214767888046186
2	19.02378778460395	21.030252787848458	34.23073501618434	25.71522441136326
3	20.674421104966527	23.181670083997016	29.049509176036807	27.09439963499965
4	14.521752940386731	15.400144188237144	34.03055240685751	36.047550464518615
5	14.530921609516206	36.99454300460562	33.812141610094706	14.662393775783473
6	35.145691789729675	34.121584193214424	15.743096074008625	14.989627943047285
7	29.342524560299555	30.411547720466924	21.197308122403914	19.048619596829607
8	27.81419473707477	32.71075523091675	20.018042631036927	19.457007400971552
9	27.08419403305196	13.598719005941625	19.86506775264459	39.452019208361826
10	17.369989090375242	26.520593758646793	32.46085442172713	23.648562729250838
11	35.595775208246124	21.774606825091812	20.957067161047128	21.672550805614936
12	21.95001219760447	25.66550621150046	28.872357390356633	23.51212420053844
13	30.253169876337576	19.75657183461249	25.22437316046754	24.765885128582397
14	23.364988891174992	20.985664676665248	25.07221691324747	30.577129518912287
15	26.152482608180744	27.11361017984235	22.553889125685536	24.180018086291366
16	25.38291473083134	26.105438603480714	24.128935501141445	24.382711164546503
17	23.662370308356532	26.25557556047583	25.505491105026557	24.576563026141077
18	23.59180430130648	24.623497880018142	27.218176668247533	24.566521150427846
19	24.18776779472223	25.146985226981865	26.977881131479258	23.687365846816643
20	24.875145102375285	24.35880713431609	26.93836853356415	23.827679229744472
21	25.354535516859162	24.910564544429022	25.89908897265608	23.835810966055732
22	25.018951311638155	24.73079313899757	26.193523317617434	24.056732231746842
23	24.169430456463285	24.9472937963584	26.460069627309974	24.423206119868343
24	24.1693758810518	25.462321954541956	26.34720767635908	24.021094488047165
25	24.453168020773585	25.098140233702825	26.23581926151828	24.21287248400531
26	24.21483719881877	25.758721014316766	26.192759261856647	23.83368252500782
27	24.637687487004232	24.871161097336884	25.985045245744892	24.506106169913995
28	23.785765313724024	25.231522539372065	26.74047809151969	24.24223405538422
29	23.38550924589334	25.543966770123454	26.901857583280712	24.168666400702495
30	23.47730508801104	26.16809117586544	26.759197457659035	23.595406278464488
31	24.142688504835654	26.07525840092953	25.855974397582965	23.926078696651853
32	23.32880539336046	25.803254550088496	26.259341263868297	24.60859879268275
33	23.293822554598606	25.482078253499512	26.86316361653788	24.360935575364003
34	23.99615352499854	25.167560157111694	26.651028992095842	24.18525732579392
35	23.881763462526067	25.388426847391322	27.049156618878616	23.680653071203995
36	23.285145064172497	26.093049985073623	26.489376623277394	24.132428327476482
37	24.079599329159045	25.360811689179936	26.409259919217476	24.15032906244355
38	23.449635354388164	25.304708166173395	27.075134514745457	24.170521964692984
39	23.699809040635213	25.194738712031207	26.8812280777394	24.224224169594184
40	22.98890973063214	25.291173464125126	27.62017914924574	24.099737655996993
41	22.612775994677804	25.303780384178154	27.892947055847568	24.190496565296478
42	23.24148473498453	25.16423105701111	27.699695523779326	23.89458868422503
43	22.692619821680303	24.608162189390868	27.78810769038496	24.911110298543875
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	106.0
1	138.0
2	170.0
3	576.0
4	982.0
5	982.0
6	1445.0
7	1908.0
8	1879.5
9	1851.0
10	2682.5
11	3514.0
12	3514.0
13	6193.5
14	8873.0
15	12513.0
16	16153.0
17	15804.5
18	15456.0
19	15456.0
20	17796.5
21	20137.0
22	18897.5
23	17658.0
24	20294.5
25	22931.0
26	22931.0
27	27039.5
28	31148.0
29	37009.0
30	42870.0
31	49045.0
32	55220.0
33	55220.0
34	60974.0
35	66728.0
36	73112.0
37	79496.0
38	86535.0
39	93574.0
40	93574.0
41	98366.0
42	103158.0
43	103728.0
44	104298.0
45	107773.0
46	111248.0
47	111248.0
48	115655.0
49	120062.0
50	122457.0
51	124852.0
52	130228.0
53	135604.0
54	135604.0
55	131000.5
56	126397.0
57	122006.5
58	117616.0
59	110031.5
60	102447.0
61	102447.0
62	95373.5
63	88300.0
64	78384.0
65	68468.0
66	59931.5
67	51395.0
68	51395.0
69	43736.5
70	36078.0
71	31326.5
72	26575.0
73	20927.0
74	15279.0
75	15279.0
76	12075.0
77	8871.0
78	7199.0
79	5527.0
80	4363.0
81	3199.0
82	3199.0
83	2394.0
84	1589.0
85	1390.5
86	1192.0
87	1031.5
88	871.0
89	871.0
90	666.5
91	462.0
92	255.0
93	48.0
94	31.0
95	14.0
96	14.0
97	8.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1832327.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.26746103144466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.99877581787219	53.54925421991301
2	9.206376547107492	11.465153857756437
3	2.1213426068798826	3.96271854324709
4	0.8007770955557038	1.9944942636955287
5	0.42861052413627077	1.3344244554661155
6	0.2701039333952758	1.0091211688278154
7	0.17408286111863555	0.7587788439662922
8	0.11451416638922148	0.5704405114551374
9	0.09570410118406049	0.5363326251925131
>10	0.618966454169138	7.615632192199233
>50	0.0886444272570427	3.9164522110449678
>100	0.07568514334139502	9.340626144006396
>500	0.005713163062859588	2.4186224380395456
>1k	4.394740817584298E-4	0.4667910656688832
>5k	2.636844490550579E-4	1.0611574595209257
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7435	0.4057681843906683	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6417	0.35021041549898024	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5537	0.30218405339221655	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3356	0.18315508094352154	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.186311722743812E-4	0.0
3	0.0	0.0	0.0	0.0015281115215788448	0.0
4	0.0	0.0	0.0	0.0028379213972178547	0.0
5	0.0	1.0915082296991748E-4	0.0	0.003165373866127607	0.0
6	0.0	1.0915082296991748E-4	0.0	0.0032745246890975247	0.0
7	0.0	1.0915082296991748E-4	0.0	0.003656552569492236	0.0
8	0.0	1.0915082296991748E-4	0.0	0.0038748542154320707	0.0
9	0.0	1.0915082296991748E-4	0.0	0.005730418205920668	0.0
10	0.0	1.0915082296991748E-4	0.0	0.00829546254571373	0.0
11	0.0	1.0915082296991748E-4	0.0	0.009659847832837697	0.0
12	5.457541148495874E-5	1.0915082296991748E-4	0.0	0.010260177359172245	0.0
13	5.457541148495874E-5	1.0915082296991748E-4	0.0	0.010805931474021832	0.0
14	5.457541148495874E-5	1.0915082296991748E-4	0.0	0.011569987234811254	0.0
15	5.457541148495874E-5	1.0915082296991748E-4	0.0	0.012716070875995387	0.0
16	5.457541148495874E-5	1.0915082296991748E-4	0.0	0.015008238158363654	0.0
17	5.457541148495874E-5	1.0915082296991748E-4	0.0	0.01702752838330713	0.0
18	5.457541148495874E-5	1.0915082296991748E-4	0.0	0.018555639904885974	0.0
19	5.457541148495874E-5	1.0915082296991748E-4	0.0	0.020465779306859527	0.0
20	5.457541148495874E-5	1.0915082296991748E-4	0.0	0.021993890828438372	0.0
21	5.457541148495874E-5	1.0915082296991748E-4	0.0	0.027178554919509455	0.0
22	5.457541148495874E-5	1.6372623445487622E-4	0.0	0.0337276042977045	0.0
23	1.0915082296991748E-4	1.6372623445487622E-4	0.0	0.04196849143193327	0.0
24	1.0915082296991748E-4	1.6372623445487622E-4	0.0	0.05435710983901891	0.0
25	1.0915082296991748E-4	1.6372623445487622E-4	0.0	0.058450265700390816	0.0
26	1.0915082296991748E-4	1.6372623445487622E-4	0.0	0.06396238226037165	0.0
27	1.0915082296991748E-4	1.6372623445487622E-4	0.0	0.07274902350945	0.0
28	1.0915082296991748E-4	1.6372623445487622E-4	0.0	0.09343310446224937	0.0
29	1.0915082296991748E-4	1.6372623445487622E-4	0.0	0.12454108900867586	0.0
30	1.6372623445487622E-4	1.6372623445487622E-4	0.0	0.1718033953546501	0.0
31	1.6372623445487622E-4	1.6372623445487622E-4	0.0	0.332036803474489	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3810	0.0	15.63517	1
GCAGTCG	310	0.0	15.5161295	9
CAGTCGG	310	0.0	15.5161295	10
AGTCGGT	300	0.0	15.416667	11
GCGCAAG	370	0.0	15.0	1
AAGACGG	335	0.0	14.910448	5
AGGACCG	480	0.0	14.645833	5
ATACCGT	280	0.0	14.535715	6
GCTATAC	90	8.2773226E-4	14.388888	3
CGCGGTT	375	0.0	14.306666	10
CGCAAGA	350	0.0	14.271429	2
GTATTGG	130	4.4471453E-6	14.230768	1
TAGGACC	585	0.0	14.230768	4
CGGTGAT	300	0.0	14.183333	14
TAACGCC	265	0.0	13.962263	4
CGTTGGA	80	0.0063005043	13.875001	2
TCGGTGA	320	0.0	13.875001	13
TACCGTC	280	0.0	13.875001	7
CAAGACG	360	0.0	13.875	4
GTCGCCA	240	5.456968E-12	13.874999	12
>>END_MODULE