FastQCFastQC Report
Fri 10 Feb 2017
ERR1630651.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630651.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74033
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14661.9801980198019802No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT13861.8721381005767697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13021.7586751853902989No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT11211.5141896181432606Illumina PCR Primer Index 10 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9471.279159293828428No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG6970.9414720462496454No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC6780.915807815433658No Hit
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC5520.7456134426539516No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4840.6537625113125228No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC2850.384963462239812Illumina PCR Primer Index 10 (95% over 23bp)
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2190.2958140288790134No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.2687990490727108No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG1870.25259006118892924No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA1240.1674928747990761No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1230.16614212580876095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG950.12832115407993733No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTC940.12697040508962218No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.12561965609930706No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.12426890710899192No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA920.12426890710899192No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA900.12156740912836168No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA890.12021666013804655No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA870.1175151621574163No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC840.1134629151864709No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA830.11211216619615577No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT820.11076141720584064No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA810.1094106682155255No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC750.10130617427363472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC507.1377144E-933.337
TTTGAGC250.00546358629.5999983
TTTTGAG250.00546358629.5999982
TGCGGCC250.00546358629.59999812
AGCTGAT551.8716673E-523.5454547
CCGTCTT1450.022.96551737
GCCGTCT1450.022.96551736
TATACAC2500.022.9399993
GGCAGTC1300.022.769238
CAGTCGG1400.021.14285910
TTATACA2800.021.1428592
AGTCGGT1400.021.14285911
GCCTTAT1251.8189894E-1120.7226
GGGCAGT1450.020.4137947
TCGGTGA1450.020.41379413
GCAGTCG1450.020.4137949
GATGGCG555.080875E-420.18181811
GATATGC1202.2737368E-1020.04166634
TGATGGC656.791283E-519.92307710
CGCCTTA1303.092282E-1119.92307725
Produced by FastQC (version 0.11.5)