##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630651.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 74033 Sequences flagged as poor quality 0 Sequence length 43 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.859414045088002 33.0 31.0 34.0 30.0 34.0 2 31.986208852808883 33.0 31.0 34.0 30.0 34.0 3 31.970783299339484 33.0 31.0 34.0 30.0 34.0 4 35.68138532816447 37.0 35.0 37.0 33.0 37.0 5 35.68534302270609 37.0 35.0 37.0 35.0 37.0 6 35.795685707724935 37.0 35.0 37.0 35.0 37.0 7 35.73129550335661 37.0 35.0 37.0 33.0 37.0 8 35.76483460078614 37.0 35.0 37.0 35.0 37.0 9 37.407304850539624 39.0 37.0 39.0 34.0 39.0 10 37.31872273175476 39.0 37.0 39.0 34.0 39.0 11 37.43707535828617 39.0 37.0 39.0 34.0 39.0 12 37.397755055178095 39.0 37.0 39.0 34.0 39.0 13 37.45395972066511 39.0 37.0 39.0 35.0 39.0 14 38.50301892399335 40.0 38.0 41.0 34.0 41.0 15 38.45343292855888 40.0 38.0 41.0 34.0 41.0 16 38.29808328718274 40.0 38.0 41.0 34.0 41.0 17 38.43013250847595 40.0 38.0 41.0 34.0 41.0 18 38.5508084232707 40.0 38.0 41.0 34.0 41.0 19 38.64114651574298 40.0 38.0 41.0 34.0 41.0 20 38.682614509745655 40.0 38.0 41.0 34.0 41.0 21 38.63458187565005 40.0 38.0 41.0 34.0 41.0 22 38.507976172787814 40.0 38.0 41.0 34.0 41.0 23 38.431793929734035 40.0 38.0 41.0 34.0 41.0 24 38.32474707225156 40.0 38.0 41.0 34.0 41.0 25 38.271581591992756 40.0 38.0 41.0 34.0 41.0 26 38.02031526481434 40.0 37.0 41.0 33.0 41.0 27 37.780057541906984 40.0 37.0 41.0 33.0 41.0 28 37.68041278889144 40.0 36.0 41.0 33.0 41.0 29 37.49744033066335 39.0 36.0 41.0 32.0 41.0 30 37.33094701011711 39.0 36.0 41.0 32.0 41.0 31 37.10423729958262 39.0 35.0 41.0 32.0 41.0 32 36.83045398673565 39.0 35.0 41.0 31.0 41.0 33 36.64664406413356 39.0 35.0 41.0 31.0 41.0 34 36.471830129806975 39.0 35.0 41.0 30.0 41.0 35 36.316075263733744 39.0 35.0 41.0 30.0 41.0 36 36.19610173841395 38.0 35.0 41.0 30.0 41.0 37 35.88661812975295 38.0 35.0 40.0 29.0 41.0 38 35.62713924871341 38.0 35.0 40.0 28.0 41.0 39 35.38774600515986 38.0 35.0 40.0 27.0 41.0 40 34.94187727094674 38.0 34.0 40.0 25.0 41.0 41 34.72845893047695 38.0 34.0 40.0 24.0 41.0 42 34.37907419664204 37.0 34.0 40.0 22.0 41.0 43 33.39258168654519 36.0 33.0 40.0 19.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 3.0 12 0.0 13 0.0 14 0.0 15 1.0 16 0.0 17 1.0 18 3.0 19 4.0 20 15.0 21 29.0 22 57.0 23 99.0 24 156.0 25 291.0 26 445.0 27 666.0 28 920.0 29 1331.0 30 1629.0 31 1975.0 32 2396.0 33 2991.0 34 3759.0 35 4864.0 36 6811.0 37 11650.0 38 15948.0 39 17989.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.74761255115961 17.22610187348885 15.774046708900086 22.25223886645145 2 18.570097118852406 21.276998095443926 34.30497210703335 25.847932678670322 3 21.010900544351845 24.652519822241434 33.621493117933895 20.71508651547283 4 12.659219537233396 18.164872421757867 28.992476328123946 40.18343171288479 5 12.505234152337472 38.43826401739765 32.29505760944444 16.761444220820447 6 30.983480339848445 30.393203031080734 17.570542866019206 21.05277376305161 7 24.687639295989626 34.51028595356125 18.152715680845027 22.649359069604095 8 33.476962975970174 28.014534059135794 19.052314508394904 19.45618845649913 9 26.78535247794902 10.714140991179608 17.936595842394606 44.56391068847676 10 20.892034633204112 27.42695824834871 23.45440546783191 28.22660165061527 11 31.48460821525536 22.09014898761363 17.632677319573702 28.792565477557307 12 21.740304999122014 31.72504153553145 23.04647927275674 23.488174192589792 13 35.15729471992219 16.364324017667798 26.114030229762403 22.364351032647605 14 25.29412559264112 21.69978252941256 25.36841678710845 27.63767509083787 15 31.512973944051975 22.002350303243148 22.54805289533046 23.936622857374417 16 25.68179055286156 22.407575000337687 26.404441262680155 25.506193184120598 17 18.82944092499291 31.997892831575108 24.65657206921238 24.516094174219603 18 22.69798603325544 19.734442748504044 28.691259303283672 28.876311914956844 19 22.342739048802564 26.10187348884957 29.197790174651843 22.357597287696027 20 25.951940350924584 19.562897626734024 31.191495684356973 23.293666337984412 21 30.489106209393107 19.531830399956775 27.143300960382533 22.835762430267582 22 26.962300595680304 25.269812110815447 26.08161225399484 21.686275039509407 23 26.2031796631232 21.784879715802415 26.76374049410398 25.248200126970406 24 22.7209487660908 24.86458741372091 28.689908554293353 23.72455526589494 25 22.245485121499872 24.006861804870802 28.376534788540248 25.371118285089082 26 25.361663042156874 21.983439817378738 29.174827441816486 23.480069698647902 27 22.095551983574893 26.75833749814272 27.699809544392366 23.446300973890022 28 22.652060567584726 21.956424837572435 31.54269042183891 23.84882417300393 29 20.344981292126484 24.322937068604542 30.429673253819246 24.902408385449732 30 22.83171018329664 23.64621182445666 32.276147123580024 21.245930868666676 31 22.183350667945376 24.418840246916915 29.050558534707495 24.347250550430214 32 19.968122323828563 27.62416760093472 30.876771169613548 21.530938905623167 33 20.689422284656843 24.50393743330677 33.399970283522215 21.40666999851418 34 20.77587022003701 26.979860332554402 28.188780678886445 24.055488768522146 35 19.310307565545095 25.529155916955954 31.912795645185255 23.2477408723137 36 19.8911296313806 25.983007577701834 29.454432482811722 24.671430308105844 37 19.46969594640228 27.397241770561777 29.33556657166399 23.797495711371955 38 19.17793416449421 24.239190631205005 31.537287425877647 25.045587778423133 39 21.171639674199344 24.942930855159183 29.677306066213717 24.208123404427756 40 18.599813596639336 23.675928302243594 34.42789026515203 23.296367835965043 41 18.62412707846501 26.105925735820513 29.850201936974052 25.419745248740426 42 18.602515094619967 24.555265894938742 33.43644050626072 23.405778504180567 43 17.363878270500994 25.604797860413598 32.72999878432591 24.301325084759497 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 6.0 2 11.0 3 59.5 4 108.0 5 108.0 6 169.0 7 230.0 8 260.0 9 290.0 10 384.0 11 478.0 12 478.0 13 819.5 14 1161.0 15 2054.0 16 2947.0 17 2536.0 18 2125.0 19 2125.0 20 2274.5 21 2424.0 22 1716.0 23 1008.0 24 925.0 25 842.0 26 842.0 27 880.5 28 919.0 29 1005.0 30 1091.0 31 1088.0 32 1085.0 33 1085.0 34 1125.0 35 1165.0 36 1246.0 37 1327.0 38 1532.5 39 1738.0 40 1738.0 41 2029.0 42 2320.0 43 2770.0 44 3220.0 45 3970.5 46 4721.0 47 4721.0 48 5532.0 49 6343.0 50 5911.5 51 5480.0 52 4700.0 53 3920.0 54 3920.0 55 4816.0 56 5712.0 57 4656.0 58 3600.0 59 4456.5 60 5313.0 61 5313.0 62 4715.5 63 4118.0 64 3398.0 65 2678.0 66 2532.5 67 2387.0 68 2387.0 69 2118.0 70 1849.0 71 1620.0 72 1391.0 73 1162.5 74 934.0 75 934.0 76 736.5 77 539.0 78 429.0 79 319.0 80 235.0 81 151.0 82 151.0 83 108.5 84 66.0 85 39.5 86 13.0 87 10.0 88 7.0 89 7.0 90 4.5 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 74033.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.68465414072103 #Duplication Level Percentage of deduplicated Percentage of total 1 86.2460857949942 53.2005997325517 2 8.215998423369172 10.136020423324734 3 2.2685965795870104 4.198127861899423 4 0.9766352070422843 2.4097361987221912 5 0.5255436091707361 1.6208987883781556 6 0.3372238158845556 1.24809206705118 7 0.2452536842796768 1.0589872084070617 8 0.1729914380187006 0.853673361879162 9 0.1554733177130094 0.863128604811368 >10 0.7532791731447216 8.111247686842354 >50 0.0700724812227648 3.1026704307538533 >100 0.0153283552674798 2.1895641133008255 >500 0.0087590601528456 3.8820525981656826 >1k 0.0087590601528456 7.125200923912309 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 1466 1.9801980198019802 No Hit CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT 1386 1.8721381005767697 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 1302 1.7586751853902989 No Hit TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT 1121 1.5141896181432606 Illumina PCR Primer Index 10 (95% over 22bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 947 1.279159293828428 No Hit ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG 697 0.9414720462496454 No Hit ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC 678 0.915807815433658 No Hit CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC 552 0.7456134426539516 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 484 0.6537625113125228 No Hit CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC 285 0.384963462239812 Illumina PCR Primer Index 10 (95% over 23bp) CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA 219 0.2958140288790134 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 199 0.2687990490727108 No Hit CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG 187 0.25259006118892924 No Hit CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA 124 0.1674928747990761 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 123 0.16614212580876095 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 95 0.12832115407993733 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTC 94 0.12697040508962218 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 93 0.12561965609930706 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.12426890710899192 No Hit GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA 92 0.12426890710899192 No Hit CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA 90 0.12156740912836168 No Hit CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA 89 0.12021666013804655 No Hit GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 87 0.1175151621574163 No Hit CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC 84 0.1134629151864709 No Hit CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA 83 0.11211216619615577 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 82 0.11076141720584064 No Hit GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA 81 0.1094106682155255 No Hit GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC 75 0.10130617427363472 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.016208987883781556 0.0 3 0.0 0.0 0.0 0.022962732835357207 0.0 4 0.0 0.0 0.0 0.033768724757878246 0.0 5 0.0 0.0 0.0 0.037820971728823635 0.0 6 0.0 0.0 0.0 0.03917172071913876 0.0 7 0.0 0.0 0.0 0.04322396769008415 0.0 8 0.0 0.0 0.0 0.04322396769008415 0.0 9 0.0 0.0 0.0 0.07023894749638675 0.0 10 0.0 0.0 0.0 0.09185093134142883 0.0 11 0.0 0.0 0.0 0.09590317831237422 0.0 12 0.0 0.0 0.0 0.0999554252833196 0.0 13 0.0 0.0 0.0 0.10265692326394986 0.0 14 0.0 0.0 0.0 0.10535842124458011 0.0 15 0.0 0.0 0.0 0.11751516215741628 0.0 16 0.0 0.0 0.0 0.14453014196371888 0.0 17 0.0 0.0 0.0 0.17424661975065173 0.0 18 0.0 0.0 0.0 0.18910485864411816 0.0 19 0.0 0.0 0.0 0.20531384652789972 0.0 20 0.0 0.0 0.0 0.21611983845042076 0.0 21 0.0 0.0 0.0 0.2674483000823957 0.0 22 0.0 0.0 0.0 0.37010522334634555 0.0 23 0.0 0.0 0.0 0.5443518430969972 0.0 24 0.0013507489903151297 0.0 0.0 0.7888374103440358 0.0 25 0.0013507489903151297 0.0 0.0 0.8361136250050654 0.0 26 0.0013507489903151297 0.0 0.0 0.9023003255305067 0.0 27 0.0013507489903151297 0.0 0.0 0.9657855280753178 0.0 28 0.0013507489903151297 0.0 0.0 1.04277822052328 0.0 29 0.0013507489903151297 0.0 0.0 1.1400321478259694 0.0 30 0.0013507489903151297 0.0 0.0 1.3656072292085961 0.0 31 0.0013507489903151297 0.0 0.0 2.009914497588913 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTTC 50 7.1377144E-9 33.3 37 TTTGAGC 25 0.005463586 29.599998 3 TTTTGAG 25 0.005463586 29.599998 2 TGCGGCC 25 0.005463586 29.599998 12 AGCTGAT 55 1.8716673E-5 23.545454 7 CCGTCTT 145 0.0 22.965517 37 GCCGTCT 145 0.0 22.965517 36 TATACAC 250 0.0 22.939999 3 GGCAGTC 130 0.0 22.76923 8 CAGTCGG 140 0.0 21.142859 10 TTATACA 280 0.0 21.142859 2 AGTCGGT 140 0.0 21.142859 11 GCCTTAT 125 1.8189894E-11 20.72 26 GGGCAGT 145 0.0 20.413794 7 TCGGTGA 145 0.0 20.413794 13 GCAGTCG 145 0.0 20.413794 9 GATGGCG 55 5.080875E-4 20.181818 11 GATATGC 120 2.2737368E-10 20.041666 34 TGATGGC 65 6.791283E-5 19.923077 10 CGCCTTA 130 3.092282E-11 19.923077 25 >>END_MODULE