FastQCFastQC Report
Fri 10 Feb 2017
ERR1630650.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630650.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1516302
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65550.4323017446392605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57030.3761124103245923No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49600.327111617606519No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33950.2238999882609137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACCG500.00703589118.59
TCTATAC1606.2937033E-1016.18753
TAACGAA1455.3485564E-815.31034513
TGCGCTA855.365555E-415.23529310
GTGTAAG1954.1836756E-1115.1794861
CGACGGT2201.8189894E-1215.1363647
GGTATCA32500.015.0846151
CGAACGA1252.9600687E-614.79999916
ACGAACG1252.9600687E-614.79999915
ATAACGA1405.997972E-714.53571512
TCTTATA14150.014.51236737
TTAGGAC1551.2107557E-714.3225813
GTAAGGC1551.2107557E-714.3225813
TTAGGCA2201.8189894E-1114.2954544
TTACACT2201.8189894E-1114.2954544
ACGACGG2201.8189894E-1114.2954546
GTGTAGG3000.014.1833331
CTATACT2252.7284841E-1113.97777754
CTAGTAG800.006299844513.8753
CGCGTAA1753.567402E-813.74285710
Produced by FastQC (version 0.11.5)