##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630650.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1516302
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09630139642367	33.0	31.0	34.0	30.0	34.0
2	32.26239034176569	34.0	31.0	34.0	30.0	34.0
3	32.36578663089543	34.0	31.0	34.0	30.0	34.0
4	35.9364077868393	37.0	35.0	37.0	35.0	37.0
5	35.89091289202283	37.0	35.0	37.0	35.0	37.0
6	35.96531957354142	37.0	35.0	37.0	35.0	37.0
7	35.9103232733321	37.0	35.0	37.0	35.0	37.0
8	35.9131729695008	37.0	35.0	37.0	35.0	37.0
9	37.6282152236164	39.0	37.0	39.0	35.0	39.0
10	37.54216904020439	39.0	37.0	39.0	35.0	39.0
11	37.637917116774894	39.0	37.0	39.0	35.0	39.0
12	37.57660743044591	39.0	37.0	39.0	35.0	39.0
13	37.62319643448337	39.0	37.0	39.0	35.0	39.0
14	38.9254159131888	40.0	38.0	41.0	36.0	41.0
15	38.929122958355265	40.0	38.0	41.0	36.0	41.0
16	38.89430535605704	40.0	38.0	41.0	35.0	41.0
17	38.88499850293675	40.0	38.0	41.0	35.0	41.0
18	38.86555778466295	40.0	38.0	41.0	35.0	41.0
19	38.92233671128839	40.0	38.0	41.0	35.0	41.0
20	38.89119251969594	40.0	38.0	41.0	35.0	41.0
21	38.85500184000285	40.0	38.0	41.0	35.0	41.0
22	38.78593578324107	40.0	38.0	41.0	35.0	41.0
23	38.72663888855914	40.0	38.0	41.0	35.0	41.0
24	38.67523488065042	40.0	38.0	41.0	34.0	41.0
25	38.62585223787873	40.0	38.0	41.0	34.0	41.0
26	38.48534131063601	40.0	38.0	41.0	34.0	41.0
27	38.35761741394524	40.0	38.0	41.0	34.0	41.0
28	38.26734054297891	40.0	38.0	41.0	34.0	41.0
29	38.18108002231745	40.0	38.0	41.0	34.0	41.0
30	38.0901284836398	40.0	37.0	41.0	33.0	41.0
31	38.03640040044793	40.0	37.0	41.0	33.0	41.0
32	37.938612492762	40.0	37.0	41.0	33.0	41.0
33	37.8492562827194	40.0	37.0	41.0	33.0	41.0
34	37.77622201909646	40.0	37.0	41.0	33.0	41.0
35	37.71805946308849	40.0	37.0	41.0	33.0	41.0
36	37.666198422214045	40.0	37.0	41.0	33.0	41.0
37	37.530303989574634	40.0	37.0	41.0	33.0	41.0
38	37.38149985952666	40.0	36.0	41.0	32.0	41.0
39	37.313059667533246	40.0	36.0	41.0	32.0	41.0
40	37.12437693810336	40.0	36.0	41.0	31.0	41.0
41	37.04901530170111	40.0	36.0	41.0	31.0	41.0
42	36.8988479867467	39.0	35.0	41.0	31.0	41.0
43	36.0984909338641	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	8.0
11	5.0
12	3.0
13	3.0
14	1.0
15	3.0
16	3.0
17	12.0
18	37.0
19	77.0
20	175.0
21	376.0
22	629.0
23	1155.0
24	1937.0
25	3228.0
26	4982.0
27	7429.0
28	11014.0
29	15396.0
30	20601.0
31	27374.0
32	35043.0
33	45784.0
34	61097.0
35	81988.0
36	116900.0
37	183020.0
38	334368.0
39	563650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.13507335609925	18.55857210502921	11.933902349268154	25.37245218960339
2	19.174874134572136	20.678598326718557	34.12031376335321	26.026213775356094
3	20.98124252292749	22.949122272476064	28.575837794845615	27.493797409750826
4	14.860166378465504	15.101081446835787	33.21337042356998	36.82538175112873
5	15.064545189546674	36.46186577607891	33.311833658466455	15.161755375907966
6	35.95886571408598	33.366440194631416	15.41328838186588	15.261405709416726
7	29.67034271536936	29.711165717647276	21.209231406408485	19.409260160574874
8	27.90941382389524	32.61850211897102	19.56378082994021	19.908303227193528
9	27.09466847633255	13.458268867283692	19.660595316764073	39.786467339619676
10	17.580204998740356	26.346796350595064	32.26125138659713	23.81174726406745
11	36.12796131641322	21.37852485850444	21.115978215421467	21.37753560966087
12	22.207449439491604	25.005902518099955	28.880064789204262	23.90658325320418
13	30.509027884946406	19.553954291427434	24.788333722437876	25.148684101188284
14	23.170648063512413	20.45872128375482	25.349435666509706	31.021194986223062
15	26.070202373933427	27.054109273746256	22.44862830755351	24.427060044766808
16	25.387159022411105	25.76294168312117	24.292917901578974	24.55698139288875
17	23.99218625313427	26.113333623512997	25.550187231831128	24.34429289152161
18	23.935139569821843	24.681494847332523	26.869977088996784	24.51338849384885
19	24.407604817509966	25.164973732145707	26.788594884132582	23.638826566211744
20	24.522291733440962	24.53924086362743	26.839376324769077	24.09909107816253
21	25.123227430947132	24.587582157116458	26.393291046242766	23.89589936569364
22	25.1110926451327	24.571226576236132	26.213577506327894	24.104103272303274
23	24.33585130138983	24.82005563535496	26.446446684103826	24.397646379151382
24	24.319231920817884	25.35332671196107	26.13753724521896	24.189904122002083
25	24.4938013667462	24.71658020631774	26.327736822875654	24.461881604060405
26	24.434248586363402	25.34831451782033	26.30590739839425	23.91152949742202
27	24.521632234211918	25.115972939427632	25.96547389636101	24.396920929999432
28	24.07963585090569	25.262711517890235	26.587249769505018	24.070402861699055
29	23.78404829644754	25.464979931438457	26.821306045893234	23.929665726220765
30	23.751996633915933	25.666852645449257	26.562188798801294	24.01896192183351
31	23.955188346384823	25.542009441390963	26.172886403895795	24.32991580832842
32	23.584483829738403	25.54471338823005	26.420330514633626	24.45047226739792
33	23.565160502327373	25.36203210178447	26.694616243993615	24.378191151894544
34	24.184562178246814	25.16688627990994	26.291332465432347	24.3572190764109
35	23.94133886257487	25.261854168892476	26.791892380277808	24.00491458825485
36	23.522820651822656	25.51127677731745	26.42006671494201	24.545835855917886
37	23.85415306449507	24.857844941179263	26.725085108375506	24.56291688595016
38	23.31850779066439	24.88903925471311	27.318172765056037	24.474280189566457
39	23.29423821903552	24.64878368557187	27.32806525349172	24.72891284190089
40	23.241016631251558	24.735837583805864	27.632160348004554	24.390985436938024
41	22.6823548343272	24.923992713852517	27.951687724477047	24.441964727343233
42	22.900055529835086	25.06479579925371	27.81820508051826	24.21694359039294
43	22.714604346627517	24.564565634022774	27.90130198337798	24.819528035971725
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	201.0
1	212.5
2	224.0
3	610.0
4	996.0
5	996.0
6	1416.5
7	1837.0
8	1899.0
9	1961.0
10	2756.0
11	3551.0
12	3551.0
13	6210.5
14	8870.0
15	11844.5
16	14819.0
17	14352.0
18	13885.0
19	13885.0
20	15625.5
21	17366.0
22	15172.0
23	12978.0
24	14794.0
25	16610.0
26	16610.0
27	19534.0
28	22458.0
29	26557.0
30	30656.0
31	34657.0
32	38658.0
33	38658.0
34	43752.0
35	48846.0
36	54761.0
37	60676.0
38	67149.0
39	73622.0
40	73622.0
41	78308.5
42	82995.0
43	86958.0
44	90921.0
45	95423.5
46	99926.0
47	99926.0
48	103682.5
49	107439.0
50	108105.0
51	108771.0
52	108982.0
53	109193.0
54	109193.0
55	105740.0
56	102287.0
57	98960.5
58	95634.0
59	91196.0
60	86758.0
61	86758.0
62	80654.0
63	74550.0
64	66506.5
65	58463.0
66	51150.0
67	43837.0
68	43837.0
69	37519.5
70	31202.0
71	26587.0
72	21972.0
73	17870.0
74	13768.0
75	13768.0
76	11019.5
77	8271.0
78	6574.0
79	4877.0
80	4074.5
81	3272.0
82	3272.0
83	2527.0
84	1782.0
85	1407.0
86	1032.0
87	871.5
88	711.0
89	711.0
90	538.0
91	365.0
92	205.0
93	45.0
94	29.5
95	14.0
96	14.0
97	7.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1516302.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.33720019707475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.44867314772175	52.85405317337509
2	9.842084619655655	12.46740167823361
3	2.8495626962633227	5.414499689020386
4	1.2079207656179136	3.060252774165824
5	0.6637594475452742	2.1020332505937387
6	0.4044930123972076	1.5371672942711867
7	0.28498737596310925	1.2635211739510122
8	0.20455943589077313	1.036497755457166
9	0.13422809222188326	0.7651468394215949
>10	0.8500681255397893	9.962525613228056
>50	0.06655228386854284	2.9120300266855024
>100	0.04123213819020809	4.659491931922722
>500	0.001461334874655454	0.6026112674592456
>1k	2.0876212495077917E-4	0.5523661151532636
>5k	2.0876212495077917E-4	0.8104014170614848
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6555	0.4323017446392605	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5703	0.3761124103245923	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4960	0.327111617606519	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3395	0.2238999882609137	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.594992290454012E-5	0.0
2	1.3189984580908024E-4	6.594992290454012E-5	0.0	7.913990748544815E-4	0.0
3	1.3189984580908024E-4	6.594992290454012E-5	0.0	0.001121148689377182	0.0
4	1.3189984580908024E-4	6.594992290454012E-5	0.0	0.002110397532945284	0.0
5	1.3189984580908024E-4	1.3189984580908024E-4	0.0	0.0027039468390861452	0.0
6	1.3189984580908024E-4	1.3189984580908024E-4	0.0	0.0030336964536088456	0.0
7	1.3189984580908024E-4	1.3189984580908024E-4	0.0	0.003693195682654247	0.0
8	1.3189984580908024E-4	1.3189984580908024E-4	0.0	0.0038910454513678672	0.0
9	1.3189984580908024E-4	1.3189984580908024E-4	0.0	0.005671693369790451	0.0
10	1.3189984580908024E-4	1.3189984580908024E-4	0.0	0.009035139437921997	0.0
11	1.3189984580908024E-4	2.637996916181605E-4	0.0	0.01042008781891734	0.0
12	1.3189984580908024E-4	2.637996916181605E-4	0.0	0.011409336662485442	0.0
13	1.3189984580908024E-4	3.2974961452270063E-4	0.0	0.012596435274767163	0.0
14	1.3189984580908024E-4	3.2974961452270063E-4	0.0	0.013453784272526186	0.0
15	1.3189984580908024E-4	3.2974961452270063E-4	0.0	0.014772782730616989	0.0
16	1.3189984580908024E-4	3.9569953742724074E-4	0.0	0.017872429107130373	0.0
17	1.9784976871362037E-4	3.9569953742724074E-4	0.0	0.02116992525235738	0.0
18	1.9784976871362037E-4	4.616494603317809E-4	0.0	0.022686773479161804	0.0
19	1.9784976871362037E-4	4.616494603317809E-4	0.0	0.025324770395343407	0.0
20	1.9784976871362037E-4	4.616494603317809E-4	0.0	0.027764917542811393	0.0
21	1.9784976871362037E-4	4.616494603317809E-4	0.0	0.03323876114388822	0.0
22	1.9784976871362037E-4	4.616494603317809E-4	0.0	0.04214200073600114	0.0
23	1.9784976871362037E-4	5.27599383236321E-4	0.0	0.05269398840072756	0.0
24	1.9784976871362037E-4	5.27599383236321E-4	0.0	0.06957716866428983	0.0
25	1.9784976871362037E-4	5.27599383236321E-4	0.0	0.0741277133447031	0.0
26	1.9784976871362037E-4	5.935493061408611E-4	0.0	0.08375640208876596	0.0
27	1.9784976871362037E-4	5.935493061408611E-4	0.0	0.09833133505066932	0.0
28	1.9784976871362037E-4	5.935493061408611E-4	0.0	0.1293277988158032	0.0
29	1.9784976871362037E-4	7.254491519499413E-4	0.0	0.17384399677636778	0.0
30	1.9784976871362037E-4	7.254491519499413E-4	0.0	0.2399917694496215	0.0
31	1.9784976871362037E-4	7.254491519499413E-4	0.0	0.4634301082502035	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTACCG	50	0.007035891	18.5	9
TCTATAC	160	6.2937033E-10	16.1875	3
TAACGAA	145	5.3485564E-8	15.310345	13
TGCGCTA	85	5.365555E-4	15.235293	10
GTGTAAG	195	4.1836756E-11	15.179486	1
CGACGGT	220	1.8189894E-12	15.136364	7
GGTATCA	3250	0.0	15.084615	1
CGAACGA	125	2.9600687E-6	14.799999	16
ACGAACG	125	2.9600687E-6	14.799999	15
ATAACGA	140	5.997972E-7	14.535715	12
TCTTATA	1415	0.0	14.512367	37
TTAGGAC	155	1.2107557E-7	14.322581	3
GTAAGGC	155	1.2107557E-7	14.322581	3
TTAGGCA	220	1.8189894E-11	14.295454	4
TTACACT	220	1.8189894E-11	14.295454	4
ACGACGG	220	1.8189894E-11	14.295454	6
GTGTAGG	300	0.0	14.183333	1
CTATACT	225	2.7284841E-11	13.9777775	4
CTAGTAG	80	0.0062998445	13.875	3
CGCGTAA	175	3.567402E-8	13.742857	10
>>END_MODULE