FastQCFastQC Report
Fri 10 Feb 2017
ERR1630649.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630649.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831534
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36100.4341373894513032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34380.41345272712841563No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25940.31195357014866504No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19280.2318606334798096No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC8580.10318279228510199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAACG551.9018387E-523.54545415
ACGCGTA652.681365E-622.7692327
TATACTG1107.4578566E-1121.8636365
CGAACGA603.72469E-521.58333416
GACGCGT759.260719E-619.73333426
CCGATAA851.2444598E-619.5882369
TAACGGC500.007033284318.536
TAACGAA701.21868245E-418.513
GTAATAC701.21868245E-418.53
GTAAACG1054.79331E-717.61904727
CGAACTA953.6039619E-617.52631424
ATAACGA752.0664002E-417.26666612
TCTAGCG650.001579437317.07692328
GTATAGG1107.801209E-716.8181821
TTTCGGA1554.0017767E-1016.70967730
TCTTTCG1005.873855E-616.65000210
TCGAGGC904.4435787E-516.44444514
CGCGTAG904.4435787E-516.44444528
AATACTG1851.8189894E-1116.05
TCACGTA1851.8189894E-1116.025
Produced by FastQC (version 0.11.5)