##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630649.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	831534
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.43057890597378	31.0	30.0	31.0	27.0	34.0
2	30.600665757503602	31.0	30.0	33.0	27.0	34.0
3	30.676044515317475	31.0	30.0	33.0	27.0	34.0
4	34.50249659063851	35.0	35.0	37.0	30.0	37.0
5	33.56493180074417	35.0	33.0	37.0	28.0	37.0
6	33.78796537483735	35.0	33.0	37.0	29.0	37.0
7	33.75811452087347	35.0	33.0	37.0	29.0	37.0
8	33.76863483633862	35.0	33.0	37.0	29.0	37.0
9	35.20331098908764	37.0	34.0	39.0	30.0	39.0
10	34.72411951886513	37.0	33.0	39.0	28.0	39.0
11	35.086844314243315	37.0	34.0	39.0	30.0	39.0
12	34.88085393982687	37.0	33.0	39.0	29.0	39.0
13	35.10070303799965	37.0	34.0	39.0	30.0	39.0
14	35.91854091354052	38.0	34.0	40.0	30.0	40.0
15	35.88891253995627	38.0	34.0	40.0	29.0	40.0
16	35.81610252857971	38.0	34.0	40.0	29.0	40.0
17	35.72906820406622	38.0	34.0	40.0	29.0	40.0
18	35.65425947706287	38.0	34.0	40.0	29.0	40.0
19	35.70273735048717	38.0	34.0	40.0	29.0	40.0
20	35.64201824579632	38.0	34.0	40.0	28.0	40.0
21	35.597637619147264	38.0	34.0	40.0	28.0	40.0
22	35.519116476295615	38.0	34.0	39.0	28.0	40.0
23	35.427950029704135	38.0	34.0	39.0	27.0	40.0
24	35.30468026562955	37.0	33.0	39.0	27.0	40.0
25	35.19309853836403	37.0	33.0	39.0	27.0	40.0
26	34.9351704199708	37.0	33.0	39.0	27.0	40.0
27	34.762614637525346	37.0	33.0	39.0	26.0	40.0
28	34.589516483992234	37.0	33.0	39.0	26.0	40.0
29	34.412695091241005	37.0	33.0	39.0	25.0	40.0
30	34.279402886712994	37.0	32.0	39.0	25.0	40.0
31	34.20569934602794	37.0	32.0	39.0	25.0	40.0
32	34.055241276965226	37.0	32.0	39.0	24.0	40.0
33	33.935655066419415	36.0	32.0	39.0	24.0	40.0
34	33.84829483821467	36.0	32.0	39.0	24.0	40.0
35	33.73977371941496	36.0	31.0	39.0	24.0	40.0
36	33.68151512746322	36.0	31.0	39.0	24.0	40.0
37	33.48051793432379	36.0	31.0	39.0	23.0	40.0
38	33.25751683034007	36.0	31.0	39.0	22.0	40.0
39	33.16555787255843	36.0	31.0	39.0	22.0	40.0
40	32.914788812002875	36.0	30.0	39.0	21.0	40.0
41	32.766634918115194	35.0	30.0	38.0	21.0	40.0
42	32.58764644620665	35.0	30.0	38.0	20.0	40.0
43	31.698934739890372	35.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	5.0
12	5.0
13	10.0
14	22.0
15	31.0
16	62.0
17	146.0
18	249.0
19	492.0
20	931.0
21	1569.0
22	2633.0
23	4093.0
24	6181.0
25	8889.0
26	12504.0
27	17328.0
28	22925.0
29	29615.0
30	38000.0
31	48311.0
32	60104.0
33	75202.0
34	92129.0
35	107886.0
36	113589.0
37	99756.0
38	65948.0
39	22916.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.15686430139959	20.46458713654523	12.074671630985625	24.303876931069563
2	20.155519798348596	21.69231805314034	33.72826607210288	24.42389607640818
3	22.038906406713377	24.548124310010174	28.241178352298284	25.171790930978165
4	15.951963479545034	15.958337241772435	33.563149552513785	34.526549726168746
5	14.396043938071083	38.30474761104176	33.50410205716182	13.795106393725332
6	35.427895912855035	34.74277660324172	15.313745439152218	14.515582044751026
7	29.238612011054265	30.552569107216303	21.87715715773498	18.33166172399445
8	27.964941902556	33.76241981686859	19.382731193192342	18.88990708738308
9	27.32936957478588	12.996101181671465	19.761068098237715	39.91346114530494
10	17.171396479278055	27.140441641592528	32.68669711641376	23.001464762715656
11	36.118787686372414	21.487636103875488	21.515897125072456	20.87767908467964
12	22.192718517823685	25.625290126441016	29.125567926266392	23.056423429468907
13	30.02955982557538	19.888302823456407	25.79581833094017	24.286319020028042
14	22.94253752702836	20.657844417666627	26.21335988666729	30.186258168637725
15	25.92870526039825	27.524430750877293	23.131705979551047	23.415158009173407
16	25.180209107504925	26.11775345325627	24.730437961646786	23.97159947759202
17	23.335786630492557	26.902808544208657	26.00098131886369	23.760423506435092
18	23.441735395065024	24.969393915342007	27.588408892480643	24.000461797112322
19	24.09678978851135	25.359035228866166	27.46718715049535	23.07698783212713
20	24.251082938280337	24.834462571584563	27.418241466975495	23.496213023159605
21	25.168303400702797	25.080513845495194	26.761503438223812	22.989679315578197
22	24.85418515659011	25.00799726770042	26.834741574006593	23.303076001702877
23	24.13322846690574	25.242744133132263	26.96353967486597	23.660487725096026
24	24.226910745682076	25.51489175427583	26.702215423542512	23.55598207649958
25	24.164616239384078	25.152669644295965	27.206103418501225	23.47661069781873
26	23.96486493637061	25.993404959989608	27.02751781646932	23.01421228717046
27	24.673314620929514	25.34664848340537	26.45219558069784	23.527841314967276
28	23.748517799632967	25.179006510858244	27.577104484001858	23.49537120550693
29	23.645936305671206	25.646215308093232	27.666938453508816	23.040909932726745
30	23.523993005697903	26.042951941832804	27.30146933258291	23.13158571988638
31	23.657601493143996	26.00747534075576	26.81742418229441	23.517498983805833
32	23.1243701400063	25.75793653656976	27.17615876199891	23.94153456142503
33	23.469274858273987	25.851258036352093	27.05866506961832	23.620802035755602
34	24.22282191708337	25.697926963900454	26.9446589075131	23.134592211503076
35	24.01489295687248	25.752404591995038	27.230997169087495	23.00170528204499
36	23.557304932810926	26.203859373158522	26.232721692678833	24.00611400135172
37	23.77497492585992	25.14581484340989	27.25420728436841	23.825002946361785
38	23.53469611585335	25.106008894404795	27.39370849538323	23.965586494358618
39	23.40902476627534	25.52631642241929	27.325401005851834	23.739257805453533
40	23.419367097436787	25.291088518328774	27.667058713173486	23.622485671060957
41	22.569492047228376	25.47219957331871	28.039262375320796	23.91904600413212
42	23.135434029155753	25.47628840191742	27.59863096397742	23.789646604949407
43	23.07458263883377	24.475126693556728	27.88857701549185	24.561713652117653
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	94.5
2	85.0
3	295.5
4	506.0
5	506.0
6	774.0
7	1042.0
8	1117.0
9	1192.0
10	1701.5
11	2211.0
12	2211.0
13	3757.5
14	5304.0
15	7423.5
16	9543.0
17	9047.5
18	8552.0
19	8552.0
20	9825.0
21	11098.0
22	10391.5
23	9685.0
24	10699.0
25	11713.0
26	11713.0
27	12990.5
28	14268.0
29	16214.0
30	18160.0
31	20881.0
32	23602.0
33	23602.0
34	26834.5
35	30067.0
36	33412.5
37	36758.0
38	40328.5
39	43899.0
40	43899.0
41	47203.5
42	50508.0
43	51643.0
44	52778.0
45	53601.0
46	54424.0
47	54424.0
48	54732.5
49	55041.0
50	55204.0
51	55367.0
52	55755.5
53	56144.0
54	56144.0
55	54534.5
56	52925.0
57	50671.0
58	48417.0
59	44666.5
60	40916.0
61	40916.0
62	38281.5
63	35647.0
64	32087.5
65	28528.0
66	25096.5
67	21665.0
68	21665.0
69	18692.0
70	15719.0
71	13823.0
72	11927.0
73	10684.0
74	9441.0
75	9441.0
76	8112.5
77	6784.0
78	5462.0
79	4140.0
80	2907.5
81	1675.0
82	1675.0
83	1247.0
84	819.0
85	642.0
86	465.0
87	352.5
88	240.0
89	240.0
90	187.0
91	134.0
92	82.0
93	30.0
94	20.0
95	10.0
96	10.0
97	5.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	831534.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.875950849897286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.89707007607926	55.894878088034346
2	6.30011999741404	7.922520706117204
3	1.6684433180538816	3.147148801853863
4	0.755193602172614	1.8993406324944866
5	0.43513420845734085	1.3679738552036362
6	0.2848295696872194	1.0745358014550601
7	0.21538864908695982	0.9479936279532223
8	0.1618113622184314	0.8139234606240817
9	0.12841316378319725	0.726668979706091
>10	0.9779800177172933	12.485784405371177
>50	0.10841316126177078	4.780297530586604
>100	0.06546984866559216	7.131315547386327
>500	9.627918901927632E-4	0.4068049814064761
>1k	7.702335121542106E-4	1.4008135818074192
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3610	0.4341373894513032	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3438	0.41345272712841563	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2594	0.31195357014866504	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1928	0.2318606334798096	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	858	0.10318279228510199	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.607789940038531E-4	0.0
3	0.0	0.0	0.0	9.620773173436083E-4	0.0
4	0.0	0.0	0.0	0.0013228563113474613	0.0
5	0.0	0.0	0.0	0.0014431159760154124	0.0
6	0.0	0.0	0.0	0.0014431159760154124	0.0
7	0.0	0.0	0.0	0.0016836353053513145	0.0
8	0.0	0.0	0.0	0.0019241546346872166	0.0
9	0.0	0.0	0.0	0.003848309269374433	0.0
10	0.0	0.0	0.0	0.006012983233397552	0.0
11	0.0	0.0	0.0	0.006734541221405258	0.0
12	0.0	0.0	0.0	0.008297916862088622	0.0
13	0.0	0.0	0.0	0.00913973451476428	0.0
14	0.0	0.0	0.0	0.009981552167439936	0.0
15	0.0	0.0	0.0	0.012386745460798957	0.0
16	0.0	0.0	0.0	0.01599453540083749	0.0
17	0.0	0.0	0.0	0.019842844670211923	0.0
18	0.0	0.0	0.0	0.021526479975563236	0.0
19	0.0	0.0	0.0	0.023450634610250452	0.0
20	0.0	0.0	0.0	0.02525452958026972	0.0
21	0.0	0.0	0.0	0.03114725314899932	0.0
22	0.0	0.0	0.0	0.039805949005091795	0.0
23	0.0	0.0	0.0	0.05459788775924977	0.0
24	0.0	0.0	0.0	0.08213735096821057	0.0
25	0.0	0.0	0.0	0.08839085353094402	0.0
26	0.0	0.0	0.0	0.09957500234506346	0.0
27	0.0	0.0	0.0	0.1100375931711752	0.0
28	0.0	0.0	0.0	0.1319248521407423	0.0
29	0.0	0.0	0.0	0.1660785969064404	0.0
30	0.0	0.0	0.0	0.21851181070166703	0.0
31	0.0	0.0	0.0	0.3945719597755474	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGAACG	55	1.9018387E-5	23.545454	15
ACGCGTA	65	2.681365E-6	22.76923	27
TATACTG	110	7.4578566E-11	21.863636	5
CGAACGA	60	3.72469E-5	21.583334	16
GACGCGT	75	9.260719E-6	19.733334	26
CCGATAA	85	1.2444598E-6	19.588236	9
TAACGGC	50	0.0070332843	18.5	36
TAACGAA	70	1.21868245E-4	18.5	13
GTAATAC	70	1.21868245E-4	18.5	3
GTAAACG	105	4.79331E-7	17.619047	27
CGAACTA	95	3.6039619E-6	17.526314	24
ATAACGA	75	2.0664002E-4	17.266666	12
TCTAGCG	65	0.0015794373	17.076923	28
GTATAGG	110	7.801209E-7	16.818182	1
TTTCGGA	155	4.0017767E-10	16.709677	30
TCTTTCG	100	5.873855E-6	16.650002	10
TCGAGGC	90	4.4435787E-5	16.444445	14
CGCGTAG	90	4.4435787E-5	16.444445	28
AATACTG	185	1.8189894E-11	16.0	5
TCACGTA	185	1.8189894E-11	16.0	25
>>END_MODULE