FastQCFastQC Report
Fri 10 Feb 2017
ERR1630648.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630648.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811545
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70740.390495405855223No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64930.35842333477777255No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56270.31061883640759685No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG38870.2145682276730636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.19723495690142945No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG34180.1886787245141578No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT33910.18718828403379437No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG32150.1774728201617956No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA30140.16637731880797882No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC27270.15053448851670811No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC24710.13640290470289174No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC23890.13187638176252867No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT23680.13071715027780154No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA22900.12641143333452937No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG22510.12425857486289328No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG22070.12182970889489358No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT21420.11824161144216676No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC21230.11719278295598508No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA20970.11575754397489435No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA20470.11299746901125833No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA19670.10858134906944074No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA19530.10780852807962264No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC19340.10675969959344096No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC19170.10582127410580472No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG19000.10488284861816846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA450.003826646420.5555557
TCTAGCG2700.019.18518628
CTAGCGG2700.019.18518629
CGCAATA3000.017.88333336
CGAACGA2250.017.26666516
GCAATAC3300.016.25757637
AAGACGG3200.016.18755
ACGAACG2450.015.85714315
TAGTTCG1302.5911686E-715.6538467
TAACGGC1557.215931E-915.51612936
TTAACGG1557.215931E-915.51612935
TACTCCG2750.015.4727275
ATACCGT2750.015.4727276
GGCGCAA4550.015.45054934
GACGGAC3250.015.369237
GTATTAG4600.015.2826091
CTTGCGC3650.015.205483
ACGGACC3300.015.1363648
CGGCGCA4800.015.03124933
GGTATCA37800.015.0251311
Produced by FastQC (version 0.11.5)