FastQCFastQC Report
Fri 10 Feb 2017
ERR1630647.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630647.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972414
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT106521.0954182066486085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT98461.0125316994613407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81460.8377090416221897No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64970.6681310635182135No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20050.2061878993926455No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18550.19076237075977925No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15680.1612481926422285No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13800.1419148634223695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTAT555.1423104E-420.1818189
TATACTT1258.572897E-917.765
AAGACGG953.6052297E-617.5263165
CCTATAC852.7224172E-517.4117643
GCAGTCG2600.017.0769259
CTTACAC1201.0406802E-716.9583323
CAGTCGG2650.016.75471710
ACCGTCG700.002592108415.8571438
TCAGTAC957.057886E-515.5789483
CAAGACG1601.0953954E-815.0312514
GTCCTAC1001.09308865E-414.81
ACCTAGG750.00410380214.81
TCGGTTG750.00410380214.832
GCGTATA750.00410380214.89
AGTCGGT2900.014.67241411
GTACCAT908.2724E-414.3888886
TACCGTC908.2724E-414.3888887
GTCCAGT1304.4419758E-614.230771
TCTATGG5200.014.230772
GGAGCAT1051.6552173E-414.0952386
Produced by FastQC (version 0.11.5)