FastQCFastQC Report
Fri 10 Feb 2017
ERR1630646.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630646.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366563
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62761.7121204267752064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59951.6354623898211222No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46761.275633383620278No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34340.936810316371265No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10280.2804429252270415No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10230.2790789032171823No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9110.24852481019633732No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA6830.18632540654676005No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6170.16832031601661926No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.15740813993774602No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC5700.15549850912394322No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT5690.1552257047219714No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5650.15413448711408406No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5640.15386168271211226No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC5500.15004242108450663No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA5090.1388574406036616No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4750.12958209093661935No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG4550.12412600289718274No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT4510.12303478528929543TruSeq Adapter, Index 11 (95% over 21bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4510.12303478528929543No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC4380.11948832806366162No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG4300.117305892847887No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG4270.11648747964197151No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3950.10775773877887294No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG3920.10693932557295745No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG3860.10530249916112647No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3810.10393847715126732No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3770.10284725954337998No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.10148323753352084No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG3670.1001192155236617No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGCAT303.5947392E-430.83333227
GGCGATA303.5947392E-430.83333235
GCGTCAA303.5947392E-430.83333226
CGATAGA303.5947392E-430.83333237
CATGAGG250.005491521729.59999810
ACATGAG250.005491521729.5999989
TACGGGT405.929266E-527.754
CGAAGCA405.929266E-527.7521
TTGTGAC358.8578276E-426.4285737
GGCGTCA358.8578276E-426.4285725
CGTCAAA358.8578276E-426.4285727
CAAAGTA358.8578276E-426.4285730
CTTTGCC451.3208117E-424.6666669
TTCGGAG451.3208117E-424.6666662
TAAAGTA400.001928692523.12526
TGTGAAT400.001928692523.12528
AGCTGAC400.001928692523.1257
GGGCCTA400.001928692523.12533
ATGCATG400.001928692523.12528
CCCGCAT400.001928692523.12515
Produced by FastQC (version 0.11.5)