##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630646.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	366563
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.64993739138975	31.0	31.0	34.0	30.0	34.0
2	31.76885010216525	31.0	31.0	34.0	30.0	34.0
3	31.80079004154811	33.0	31.0	34.0	30.0	34.0
4	35.4888764005096	37.0	35.0	37.0	33.0	37.0
5	35.47349841637045	37.0	35.0	37.0	33.0	37.0
6	35.593155883163334	37.0	35.0	37.0	33.0	37.0
7	35.52040167720146	37.0	35.0	37.0	33.0	37.0
8	35.584093320929824	37.0	35.0	37.0	33.0	37.0
9	37.13064330006029	39.0	37.0	39.0	34.0	39.0
10	37.009559066245096	39.0	37.0	39.0	33.0	39.0
11	37.17007172027728	39.0	37.0	39.0	34.0	39.0
12	37.13085335944981	39.0	37.0	39.0	33.0	39.0
13	37.190461121280656	39.0	37.0	39.0	34.0	39.0
14	38.290503951571765	40.0	38.0	41.0	34.0	41.0
15	38.25607057995488	40.0	38.0	41.0	33.0	41.0
16	38.13597662611884	40.0	37.0	41.0	33.0	41.0
17	38.22720241813822	40.0	38.0	41.0	33.0	41.0
18	38.25241227292444	40.0	38.0	41.0	33.0	41.0
19	38.37140409697651	40.0	38.0	41.0	34.0	41.0
20	38.32529469695523	40.0	38.0	41.0	34.0	41.0
21	38.28474505064614	40.0	38.0	41.0	34.0	41.0
22	38.18016275510622	40.0	38.0	41.0	34.0	41.0
23	38.06420451600407	40.0	37.0	41.0	33.0	41.0
24	37.97182203332033	40.0	37.0	41.0	33.0	41.0
25	37.87338874900085	40.0	37.0	41.0	33.0	41.0
26	37.605153820762055	39.0	36.0	41.0	33.0	41.0
27	37.370263774576266	39.0	36.0	41.0	32.0	41.0
28	37.185343310699665	39.0	35.0	41.0	32.0	41.0
29	37.04291213243017	39.0	35.0	40.0	31.0	41.0
30	36.79849575652753	39.0	35.0	40.0	31.0	41.0
31	36.613534371990625	39.0	35.0	40.0	31.0	41.0
32	36.36618262072277	38.0	35.0	40.0	30.0	41.0
33	36.14904668501731	38.0	35.0	40.0	30.0	41.0
34	35.972664998922426	38.0	35.0	40.0	30.0	41.0
35	35.7436920802154	38.0	35.0	40.0	29.0	41.0
36	35.576779434912964	38.0	35.0	40.0	28.0	41.0
37	35.24257221814531	38.0	35.0	40.0	26.0	41.0
38	34.919383571173306	38.0	34.0	40.0	24.0	41.0
39	34.63214236024913	38.0	34.0	40.0	23.0	41.0
40	34.22304215100815	38.0	33.0	40.0	21.0	41.0
41	33.92688569222753	37.0	33.0	40.0	18.0	41.0
42	33.52752460013695	37.0	33.0	40.0	15.0	41.0
43	32.672533780005075	36.0	32.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	2.0
12	1.0
13	1.0
14	1.0
15	3.0
16	4.0
17	9.0
18	22.0
19	75.0
20	148.0
21	267.0
22	480.0
23	788.0
24	1259.0
25	1896.0
26	2688.0
27	4022.0
28	5521.0
29	7202.0
30	9042.0
31	11115.0
32	13645.0
33	16874.0
34	21227.0
35	26931.0
36	37238.0
37	58983.0
38	84588.0
39	62525.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.10121588921959	21.111787059795997	13.013315582860244	19.773681468124167
2	19.06275319658558	23.17964442674247	33.50638225898413	24.25122011768782
3	22.990045367372048	27.43211944467936	28.78031879922414	20.79751638872445
4	13.939486527554608	17.035543685532907	33.33997157378131	35.684998213131166
5	11.923189192580812	42.129183796509736	32.05751808011174	13.890108930797707
6	31.031500724295686	33.61686804178272	17.42183471872502	17.929796515196568
7	26.1079268775081	33.72380736735568	22.565834522305853	17.602431232830373
8	30.098509669552026	30.67467256651653	20.144422650403886	19.082395113527554
9	27.935443566317385	12.415055529336021	23.00068473904895	36.648816165297646
10	20.40877011591459	28.89926151848386	29.85107607696358	20.840892288637967
11	30.916922875467517	24.44518404748979	20.59836917528501	24.03952390175768
12	21.62384092229712	30.814075615924136	27.44521405597401	20.11686940580473
13	28.72002902638837	20.88426818855149	29.478970872674005	20.916731912386137
14	22.41933855844698	23.167913837457682	29.432866928740765	24.979880675354575
15	26.309256526163306	26.37200153861683	28.059023960410624	19.25971797480924
16	22.361776829630923	25.91750940493176	30.130700588984705	21.590013176452615
17	21.15870941693515	28.476687499829495	28.870344251874847	21.4942588313605
18	19.108038727312902	24.71225955702021	33.178744172215964	23.00095754345092
19	20.18970818113121	26.44402190073739	33.891582074568355	19.474687843563043
20	20.985205817281066	24.179199755567257	35.388459828187784	19.44713459896389
21	21.8884611922098	25.95979408723739	33.107542223301316	19.044202497251494
22	22.765254540147257	25.88286324588134	31.97349432430442	19.378387889666985
23	20.865444684815433	25.745915436091476	33.25540220917005	20.133237669923044
24	19.273903803711775	28.15641513191457	32.02259911665934	20.547081947714307
25	19.77504549013403	27.01090944803485	33.21884641930582	19.995198642525295
26	19.7859576662129	28.16078000234612	32.59903481802582	19.454227513415155
27	20.31901746766586	27.88988523118809	32.284764146954274	19.506333154191775
28	18.992096856474877	27.703014215837385	33.99879420454329	19.30609472314445
29	19.407577960677973	28.054931894381046	33.73581076104244	18.801679383898538
30	17.79339431421065	29.064853790480765	33.89376450978413	19.247987385524453
31	19.385480804118256	29.354299260972876	32.22583839612836	19.034381538780508
32	17.88969426810671	29.073856335745834	32.8249168628585	20.211532533288956
33	17.56969470459375	28.96309774854527	32.98559865561991	20.48160889124107
34	19.161235585697412	28.497147829977386	33.09035554597709	19.251261038348115
35	17.921885187539385	29.89063271524949	32.442172286892024	19.7453098103191
36	18.79104001222164	29.58618300264893	31.15098905235935	20.471787932770084
37	18.42029882994192	28.45186229925006	32.47927368556019	20.64856518524783
38	18.334638247722765	27.855239072137667	32.17536958176357	21.634753098375995
39	18.432302223628678	28.99883512520358	32.46426944345174	20.104593207715997
40	17.799123206652062	28.93445328633823	32.19419308549963	21.07223042151008
41	17.879054896429807	28.69929589183851	32.05970051532751	21.361948696404166
42	17.600248797614597	29.45796493372217	32.21056134961794	20.731224919045292
43	17.53477574114136	27.741479636515415	31.714602946833146	23.009141675510076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	336.0
1	233.0
2	130.0
3	430.0
4	730.0
5	730.0
6	1191.0
7	1652.0
8	1788.5
9	1925.0
10	2878.0
11	3831.0
12	3831.0
13	6658.0
14	9485.0
15	12673.0
16	15861.0
17	14386.0
18	12911.0
19	12911.0
20	13407.0
21	13903.0
22	10120.0
23	6337.0
24	5705.0
25	5073.0
26	5073.0
27	5366.0
28	5659.0
29	6359.5
30	7060.0
31	8269.5
32	9479.0
33	9479.0
34	11001.0
35	12523.0
36	13732.0
37	14941.0
38	17399.5
39	19858.0
40	19858.0
41	21252.0
42	22646.0
43	24479.0
44	26312.0
45	26038.5
46	25765.0
47	25765.0
48	26582.0
49	27399.0
50	26206.5
51	25014.0
52	23025.5
53	21037.0
54	21037.0
55	19354.0
56	17671.0
57	15208.0
58	12745.0
59	11820.0
60	10895.0
61	10895.0
62	10268.5
63	9642.0
64	8843.0
65	8044.0
66	7532.0
67	7020.0
68	7020.0
69	5537.5
70	4055.0
71	3526.5
72	2998.0
73	2228.5
74	1459.0
75	1459.0
76	1128.0
77	797.0
78	632.0
79	467.0
80	377.5
81	288.0
82	288.0
83	264.5
84	241.0
85	218.0
86	195.0
87	150.5
88	106.0
89	106.0
90	80.5
91	55.0
92	34.5
93	14.0
94	8.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	366563.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.52465053096716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.56606072012441	27.124657899570405
2	8.738873608623052	6.034131147440053
3	3.3699144077034724	3.490353517357006
4	1.8865660547140994	2.6053213497035896
5	1.271384972312304	2.194706092970282
6	0.8970831948578204	1.8582890279801854
7	0.625924398973969	1.5126874793367353
8	0.5244804846474708	1.448600435421297
9	0.4151419516855389	1.2899367722418194
>10	3.10483555079548	21.2865923770021
>50	0.3557210836823198	8.504351771954505
>100	0.2313375421720209	14.842134317559738
>500	0.007922518567534962	1.6725881604754214
>1k	0.003169007427013985	2.779258920456379
>5k	0.0015845037135069925	3.356390730530482
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6276	1.7121204267752064	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5995	1.6354623898211222	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4676	1.275633383620278	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3434	0.936810316371265	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1028	0.2804429252270415	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1023	0.2790789032171823	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	911	0.24852481019633732	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	683	0.18632540654676005	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	617	0.16832031601661926	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	577	0.15740813993774602	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	570	0.15549850912394322	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	569	0.1552257047219714	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	565	0.15413448711408406	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	564	0.15386168271211226	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	550	0.15004242108450663	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	509	0.1388574406036616	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	475	0.12958209093661935	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	455	0.12412600289718274	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	451	0.12303478528929543	TruSeq Adapter, Index 11 (95% over 21bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	451	0.12303478528929543	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	438	0.11948832806366162	No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTG	430	0.117305892847887	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG	427	0.11648747964197151	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	395	0.10775773877887294	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	392	0.10693932557295745	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGG	386	0.10530249916112647	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	381	0.10393847715126732	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	377	0.10284725954337998	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	372	0.10148323753352084	No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG	367	0.1001192155236617	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.456088039436604E-4	0.0
3	0.0	0.0	0.0	0.0016368264118309813	0.0
4	0.0	0.0	0.0	0.002455239617746472	0.0
5	0.0	0.0	0.0	0.002455239617746472	0.0
6	0.0	0.0	0.0	0.002455239617746472	0.0
7	0.0	0.0	0.0	0.0027280440197183023	0.0
8	0.0	0.0	0.0	0.0032736528236619626	0.0
9	0.0	0.0	0.0	0.005728892441408434	0.0
10	0.0	0.0	0.0	0.008184132059154907	0.0
11	0.0	0.0	0.0	0.010093762872957717	0.0
12	0.0	0.0	0.0	0.0117305892847887	0.0
13	0.0	0.0	0.0	0.015822655314366153	0.0
14	0.0	0.0	0.0	0.020187525745915435	0.0
15	0.0	0.0	0.0	0.028917266609014003	0.0
16	0.0	0.0	0.0	0.04364870431549284	0.0
17	0.0	0.0	0.0	0.07011073130676036	0.0
18	0.0	0.0	0.0	0.07938608097380259	0.0
19	0.0	0.0	0.0	0.09793678030788705	0.0
20	0.0	0.0	0.0	0.10693932557295745	0.0
21	0.0	0.0	0.0	0.13585659218197144	0.0
22	0.0	0.0	0.0	0.18168773171323893	0.0
23	0.0	0.0	0.0	0.2463423749805627	0.0
24	0.0	0.0	0.0	0.36228424581859053	0.0
25	0.0	0.0	0.0	0.393656752045351	0.0
26	0.0	0.0	0.0	0.4484904368416889	0.0
27	0.0	0.0	0.0	0.49677681599070284	0.0
28	0.0	0.0	0.0	0.570434004523097	0.0
29	0.0	0.0	0.0	0.6760093080861953	0.0
30	0.0	0.0	0.0	0.8500585165442229	0.0
31	0.0	0.0	0.0	1.3408336356915456	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATGCAT	30	3.5947392E-4	30.833332	27
GGCGATA	30	3.5947392E-4	30.833332	35
GCGTCAA	30	3.5947392E-4	30.833332	26
CGATAGA	30	3.5947392E-4	30.833332	37
CATGAGG	25	0.0054915217	29.599998	10
ACATGAG	25	0.0054915217	29.599998	9
TACGGGT	40	5.929266E-5	27.75	4
CGAAGCA	40	5.929266E-5	27.75	21
TTGTGAC	35	8.8578276E-4	26.42857	37
GGCGTCA	35	8.8578276E-4	26.42857	25
CGTCAAA	35	8.8578276E-4	26.42857	27
CAAAGTA	35	8.8578276E-4	26.42857	30
CTTTGCC	45	1.3208117E-4	24.666666	9
TTCGGAG	45	1.3208117E-4	24.666666	2
TAAAGTA	40	0.0019286925	23.125	26
TGTGAAT	40	0.0019286925	23.125	28
AGCTGAC	40	0.0019286925	23.125	7
GGGCCTA	40	0.0019286925	23.125	33
ATGCATG	40	0.0019286925	23.125	28
CCCGCAT	40	0.0019286925	23.125	15
>>END_MODULE