FastQCFastQC Report
Fri 10 Feb 2017
ERR1630645.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630645.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415262
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68040.2817085682629876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63910.26460897409887624No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC52150.21591860427564383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51980.21521474688874334No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT42740.17695802774191785No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA41370.17128576527101408No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA40240.16660718381691095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35680.1477272444976984No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG33270.13774903095399174No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG28530.11812383087217866No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG27630.11439752705917619No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA26480.10963613885367303No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG26220.1085596510854723No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG24790.10263896836036836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27300.019.3809531
AAGACGG3300.018.55
TATCCCG650.001580615117.0769235
CGAACGA1851.8189894E-1217.016
ACGAACG1851.8189894E-1217.015
ACGGACC3500.016.9142868
CGAACGT1107.816361E-716.8181824
GACGGAC3850.016.3376627
CGGACCA3600.015.9305559
AGACGGA3850.015.8571426
CGCAAGA3750.015.7866682
TACGGAT1557.219569E-915.5161297
GCGCAAG3950.015.4556961
CTTATAC15000.015.17000137
TCCGAAA3450.015.01449321
GTAAACG1853.0559022E-1015.027
ACGCTTC2109.094947E-1214.976191531
TAATACT5350.014.8691594
TCCGATA2251.8189894E-1214.88
GACGGTA2750.014.88
Produced by FastQC (version 0.11.5)