##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630645.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2415262
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17596765899518	33.0	31.0	34.0	30.0	34.0
2	32.35537097010594	34.0	31.0	34.0	30.0	34.0
3	32.470521624569095	34.0	31.0	34.0	30.0	34.0
4	36.02309852926929	37.0	35.0	37.0	35.0	37.0
5	35.976031585807256	37.0	35.0	37.0	35.0	37.0
6	36.04781054808961	37.0	35.0	37.0	35.0	37.0
7	36.003169428409834	37.0	35.0	37.0	35.0	37.0
8	35.9972855946891	37.0	35.0	37.0	35.0	37.0
9	37.72716790145334	39.0	37.0	39.0	35.0	39.0
10	37.652195496803245	39.0	37.0	39.0	35.0	39.0
11	37.73395971120318	39.0	37.0	39.0	35.0	39.0
12	37.69180776247049	39.0	37.0	39.0	35.0	39.0
13	37.72667188901246	39.0	37.0	39.0	35.0	39.0
14	39.05956952082217	40.0	38.0	41.0	36.0	41.0
15	39.07728768141924	40.0	38.0	41.0	36.0	41.0
16	39.05481351505551	40.0	38.0	41.0	36.0	41.0
17	39.044071409230135	40.0	38.0	41.0	36.0	41.0
18	39.0317373436091	40.0	38.0	41.0	36.0	41.0
19	39.08236828965139	40.0	39.0	41.0	36.0	41.0
20	39.057767231877946	40.0	39.0	41.0	36.0	41.0
21	39.01656010817874	40.0	39.0	41.0	36.0	41.0
22	38.952983154622565	40.0	38.0	41.0	35.0	41.0
23	38.924130384198484	40.0	38.0	41.0	35.0	41.0
24	38.87862269186531	40.0	38.0	41.0	35.0	41.0
25	38.83635978208575	40.0	38.0	41.0	35.0	41.0
26	38.728399651880416	40.0	38.0	41.0	35.0	41.0
27	38.616085128652706	40.0	38.0	41.0	34.0	41.0
28	38.53288959955483	40.0	38.0	41.0	34.0	41.0
29	38.4841636228285	40.0	38.0	41.0	34.0	41.0
30	38.427029034531245	40.0	38.0	41.0	34.0	41.0
31	38.39671348284368	40.0	38.0	41.0	34.0	41.0
32	38.33042543624667	40.0	38.0	41.0	34.0	41.0
33	38.26594630313399	40.0	38.0	41.0	34.0	41.0
34	38.22425972834417	40.0	38.0	41.0	34.0	41.0
35	38.17350291603975	40.0	38.0	41.0	34.0	41.0
36	38.157167214157305	40.0	38.0	41.0	34.0	41.0
37	38.05156417813057	40.0	38.0	41.0	33.0	41.0
38	37.933254032067744	40.0	37.0	41.0	33.0	41.0
39	37.898213527145295	40.0	37.0	41.0	33.0	41.0
40	37.7413369646854	40.0	37.0	41.0	33.0	41.0
41	37.711365889083666	40.0	37.0	41.0	33.0	41.0
42	37.59877644744131	40.0	37.0	41.0	33.0	41.0
43	36.85711819256048	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	7.0
11	7.0
12	5.0
13	2.0
14	3.0
15	6.0
16	5.0
17	19.0
18	39.0
19	75.0
20	137.0
21	363.0
22	654.0
23	1178.0
24	2063.0
25	3417.0
26	5364.0
27	8501.0
28	12858.0
29	18728.0
30	26109.0
31	35571.0
32	47536.0
33	63319.0
34	86768.0
35	120148.0
36	172923.0
37	274938.0
38	536723.0
39	997791.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.24426832368496	18.96688640818263	11.873991310259509	24.914853957872893
2	18.626178029547106	20.43244169783651	34.568754859721224	26.37262541289516
3	20.409421420947293	23.089875963767078	29.02302110495673	27.4776815103289
4	14.091390499250183	14.737324563546315	35.159332610706414	36.01195232649708
5	14.317494333948035	36.99971266057264	34.70882247971442	13.973970525764907
6	35.70689225433928	34.871330729337025	15.204685868448225	14.217091147875468
7	29.58755613262661	30.00204532675958	21.509840340302624	18.90055820031119
8	28.164356496313857	32.532826666423766	19.950133774306885	19.35268306295549
9	26.698842610035683	13.698348253729822	19.83677133164021	39.76603780459428
10	16.999315188165923	26.812370666205158	33.20968905236782	22.978625093261105
11	35.91548246111602	21.676074893738235	21.686053107281943	20.7223895378638
12	22.96297461724649	25.274193855573436	28.803293390116686	22.95953813706339
13	30.846218753907443	19.679065873598805	24.945368245763813	24.529347126729935
14	22.824977166038302	20.527131218062472	25.58368408893114	31.06420752696809
15	25.53772634190411	28.174997163868763	22.360224273805493	23.927052220421636
16	25.061380504475288	26.4276919025762	24.06852755518863	24.442400037759878
17	24.01134949334689	26.2713113525572	25.225420678998802	24.491918475097112
18	23.461305647172026	25.128288359606536	27.273148834370765	24.137257158850677
19	24.94176615207791	24.972487456847333	27.034209953205906	23.05153643786885
20	25.54306737736941	24.723032118254665	25.86957439813983	23.864326106236096
21	25.163439825575857	24.798717489034317	26.14221562712451	23.895627058265315
22	25.12108417223473	25.09214321262041	26.360535627190757	23.426236987954102
23	24.65484075847672	24.970251674559528	25.85922355421482	24.515684012748928
24	24.137671192607677	25.46514622430196	26.520476867519964	23.876705715570402
25	24.476889049718	25.58716197248994	25.819062279785797	24.11688669800626
26	24.333095125911804	25.690504798237214	26.06624043271496	23.910159643136026
27	24.45689121925489	24.68208417968734	26.29255128429131	24.56847331676646
28	23.976156624001867	25.384078414681305	26.23330305366457	24.406461907652254
29	24.147649406151384	25.823989281494097	26.542958900525075	23.485402411829444
30	24.386050043432142	25.6780837855272	25.64040671364018	24.295459457400483
31	23.56295093451559	26.29101935939041	26.402849877156186	23.743179828937812
32	23.372122775914168	25.508412751908487	26.15840434702322	24.961060125154123
33	22.909688472720557	26.017674273018827	26.603946072931215	24.468691181329397
34	24.87692846573167	24.53448114531674	26.367574201059764	24.22101618789183
35	23.848841243724284	24.87096637963086	26.546850817840877	24.73334155880397
36	23.161172576722524	25.878269107036832	26.770884483753733	24.18967383248691
37	23.867182939159395	24.53232816978034	27.27016779132036	24.330321099739905
38	23.945683739486647	25.01459468993426	26.617112346403825	24.422609224175265
39	23.670020064075864	24.604949690758186	27.193488739523914	24.53154150564204
40	22.893499752821846	24.378804452684637	27.75748552330969	24.97021027118383
41	22.014837313715862	24.766836889745296	28.283432604827137	24.93489319171171
42	22.584506359972544	24.59480586371168	28.664178047764587	24.156509728551185
43	22.944508711684282	24.318024297156995	28.15495793002995	24.58250906112877
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	406.0
1	397.0
2	388.0
3	1048.5
4	1709.0
5	1709.0
6	2136.5
7	2564.0
8	2357.5
9	2151.0
10	3160.0
11	4169.0
12	4169.0
13	6836.0
14	9503.0
15	13066.5
16	16630.0
17	16944.5
18	17259.0
19	17259.0
20	20711.5
21	24164.0
22	25035.5
23	25907.0
24	30536.0
25	35165.0
26	35165.0
27	40986.5
28	46808.0
29	53787.0
30	60766.0
31	68225.5
32	75685.0
33	75685.0
34	84457.5
35	93230.0
36	101186.0
37	109142.0
38	116614.0
39	124086.0
40	124086.0
41	128354.5
42	132623.0
43	134076.0
44	135529.0
45	139075.5
46	142622.0
47	142622.0
48	149900.5
49	157179.0
50	157038.5
51	156898.0
52	158184.0
53	159470.0
54	159470.0
55	154195.0
56	148920.0
57	147072.0
58	145224.0
59	147564.0
60	149904.0
61	149904.0
62	137359.5
63	124815.0
64	114685.0
65	104555.0
66	90116.5
67	75678.0
68	75678.0
69	66835.0
70	57992.0
71	48498.0
72	39004.0
73	27958.0
74	16912.0
75	16912.0
76	12266.5
77	7621.0
78	5975.0
79	4329.0
80	3513.0
81	2697.0
82	2697.0
83	2048.0
84	1399.0
85	1184.0
86	969.0
87	866.5
88	764.0
89	764.0
90	571.0
91	378.0
92	209.0
93	40.0
94	23.0
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2415262.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.25101712347451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.95083928094682	51.51925667135862
2	8.426216462797763	9.985237918466664
3	1.9395327722143623	3.447578684940396
4	0.7949719209987322	1.8841157961510924
5	0.42243825471177493	1.2514948131769033
6	0.2596838329708381	0.9231918740426772
7	0.17200150342908824	0.7133884817458187
8	0.1313175827592387	0.6224560275744747
9	0.10146917168604022	0.5410936466166911
>10	0.6348507465117585	7.402893418947462
>50	0.08130103726635086	3.3399260218552946
>100	0.0710957919187433	8.870709935411623
>500	0.009451086477650307	3.874940566199038
>1k	0.004550523118868515	4.644444959916897
>5k	2.800321919303702E-4	0.9792711835962211
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6804	0.2817085682629876	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6391	0.26460897409887624	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	5215	0.21591860427564383	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5198	0.21521474688874334	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	4274	0.17695802774191785	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	4137	0.17128576527101408	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	4024	0.16660718381691095	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3568	0.1477272444976984	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	3327	0.13774903095399174	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	2853	0.11812383087217866	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	2763	0.11439752705917619	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	2648	0.10963613885367303	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	2622	0.1085596510854723	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	2479	0.10263896836036836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.89823629900193E-4	0.0
3	0.0	0.0	0.0	4.554371327003033E-4	0.0
4	0.0	0.0	0.0	8.280675140005515E-4	0.0
5	4.1403375700027576E-5	0.0	0.0	9.936810168006617E-4	0.0
6	4.1403375700027576E-5	0.0	0.0	9.936810168006617E-4	0.0
7	4.1403375700027576E-5	0.0	0.0	0.0012006978953007996	0.0
8	4.1403375700027576E-5	0.0	0.0	0.0012835046467008547	0.0
9	4.1403375700027576E-5	0.0	0.0	0.002359992414901572	0.0
10	4.1403375700027576E-5	0.0	0.0	0.003270866680302178	0.0
11	4.1403375700027576E-5	0.0	0.0	0.0037263038130024816	0.0
12	4.1403375700027576E-5	0.0	0.0	0.00426454769710284	0.0
13	4.1403375700027576E-5	0.0	0.0	0.004637178078403088	0.0
14	4.1403375700027576E-5	0.0	0.0	0.004844194956903226	0.0
15	4.1403375700027576E-5	0.0	0.0	0.005672262470903778	0.0
16	4.1403375700027576E-5	0.0	0.0	0.007079977244704715	0.0
17	4.1403375700027576E-5	0.0	0.0	0.009067339278306039	0.0
18	4.1403375700027576E-5	0.0	0.0	0.01022663379790681	0.0
19	4.1403375700027576E-5	0.0	0.0	0.011634348571707748	0.0
20	4.1403375700027576E-5	0.0	0.0	0.012917853218408604	0.0
21	4.1403375700027576E-5	0.0	0.0	0.015650476014610424	0.0
22	4.1403375700027576E-5	0.0	0.0	0.021281335109814174	0.0
23	4.1403375700027576E-5	0.0	0.0	0.029313589995619522	0.0
24	4.1403375700027576E-5	0.0	0.0	0.043763368114929146	0.0
25	4.1403375700027576E-5	0.0	0.0	0.04757247867933168	0.0
26	4.1403375700027576E-5	0.0	0.0	0.05262369051473505	0.0
27	4.1403375700027576E-5	0.0	0.0	0.06256050068274166	0.0
28	4.1403375700027576E-5	0.0	0.0	0.09187409067836119	0.0
29	4.1403375700027576E-5	0.0	0.0	0.14437357106599616	0.0
30	4.1403375700027576E-5	0.0	0.0	0.2093354675393394	0.0
31	4.1403375700027576E-5	0.0	0.0	0.4627655301992082	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2730	0.0	19.380953	1
AAGACGG	330	0.0	18.5	5
TATCCCG	65	0.0015806151	17.076923	5
CGAACGA	185	1.8189894E-12	17.0	16
ACGAACG	185	1.8189894E-12	17.0	15
ACGGACC	350	0.0	16.914286	8
CGAACGT	110	7.816361E-7	16.818182	4
GACGGAC	385	0.0	16.337662	7
CGGACCA	360	0.0	15.930555	9
AGACGGA	385	0.0	15.857142	6
CGCAAGA	375	0.0	15.786668	2
TACGGAT	155	7.219569E-9	15.516129	7
GCGCAAG	395	0.0	15.455696	1
CTTATAC	1500	0.0	15.170001	37
TCCGAAA	345	0.0	15.014493	21
GTAAACG	185	3.0559022E-10	15.0	27
ACGCTTC	210	9.094947E-12	14.9761915	31
TAATACT	535	0.0	14.869159	4
TCCGATA	225	1.8189894E-12	14.8	8
GACGGTA	275	0.0	14.8	8
>>END_MODULE