FastQCFastQC Report
Fri 10 Feb 2017
ERR1630644.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630644.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2216227
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC131470.5932154061835724No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT82920.3741493989559734No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG57740.26053287862660274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52710.23783664759972692No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG49220.22208916324907152No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT48940.22082575476248598No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT48060.2168550423760743No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG46620.21035751301649155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46430.20950020011487994No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG43920.19817464546727387No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC41280.18626250830803884No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT41000.18499909982145332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40120.18102838743504163No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG38780.17498207539209656No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT38570.1740345190271574No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG37920.17110160646901243No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT35240.1590089823831223No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT34880.15738460004322663No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG33270.1501200012453598No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT32860.14827001024714526No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC32270.1456078280789829No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG32040.14457002825071621No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG31350.1414566287659161No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA27230.12286647532044326No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA26520.11966283237231565No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG25990.11727138059413589No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA25510.11510553747427496No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25010.11284945089108651No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC24840.11208238145280244No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT24300.1096458079429589No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA24070.10860800811469222No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT23670.10680313884814147No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG23520.10612631287318491No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC22750.1026519395350747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGTC502.7032E-422.1999997
CTTACCG450.003826912520.5555559
AAGACGG3850.019.70135
CCAGGAC33250.019.6406023
GACAGGC30950.019.4862677
CAGGACA34100.019.476544
TGCATCA31100.019.2733114
ACAGGCT31850.018.9356358
GCTGCAT31500.018.8523812
TCCAGGA35050.018.473612
GCATCAG32650.018.47166815
GGACAGG33900.018.281716
CGAAAGC4050.018.27160519
GAGCGAA3850.018.2597416
CAAGACG4500.018.0888884
CTGCATC33350.017.91754213
GGCCATC33600.017.67410926
ACGGACC3900.017.5512838
GACGGAC3900.017.5512837
AGCGAAA4050.017.35802517
Produced by FastQC (version 0.11.5)