FastQCFastQC Report
Fri 10 Feb 2017
ERR1630643.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630643.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2201723
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40960.18603611807661544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40180.18249343809371116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34220.15542372950639113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23050.016.37311
TAATAGT2109.094947E-1214.976194
TAGGACA5700.014.9298244
GTATTAG2750.014.81
TTAGGAC5650.014.7345133
GTGTAGG3750.014.3066661
GTTAGAC1304.4481258E-614.2307693
TAACACA2750.014.1272744
GGTATAC1458.9198693E-714.0344831
GGCGTAT800.00630103513.8750011
TAACCCG2405.456968E-1213.87500128
ACGCTAA800.00630103513.87500134
GCTTAGG5350.013.8317761
TCTTATA14750.013.671185537
CTTAGGA6550.013.2748092
CTTATAC9500.013.242105537
TACACTA1307.003491E-512.8076935
GTCTAGA1901.0471558E-712.6578951
TATACAA6150.012.6341465
TTCTATA2204.089088E-912.6136362
Produced by FastQC (version 0.11.5)