##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630643.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2201723
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.079332867940245	33.0	31.0	34.0	30.0	34.0
2	32.24943646407836	34.0	31.0	34.0	30.0	34.0
3	32.3570521814052	34.0	31.0	34.0	30.0	34.0
4	35.93061207063741	37.0	35.0	37.0	35.0	37.0
5	35.88156820817151	37.0	35.0	37.0	35.0	37.0
6	35.947269479403175	37.0	35.0	37.0	35.0	37.0
7	35.898675718971006	37.0	35.0	37.0	35.0	37.0
8	35.89426462820255	37.0	35.0	37.0	35.0	37.0
9	37.62627769251627	39.0	37.0	39.0	35.0	39.0
10	37.52752685056204	39.0	37.0	39.0	35.0	39.0
11	37.6234408233915	39.0	37.0	39.0	35.0	39.0
12	37.554403074319524	39.0	37.0	39.0	35.0	39.0
13	37.59843540717883	39.0	37.0	39.0	35.0	39.0
14	38.91106919444453	40.0	38.0	41.0	36.0	41.0
15	38.93215313642997	40.0	38.0	41.0	36.0	41.0
16	38.89228935701721	40.0	38.0	41.0	35.0	41.0
17	38.8623310016746	40.0	38.0	41.0	35.0	41.0
18	38.83854871843552	40.0	38.0	41.0	35.0	41.0
19	38.887102055980705	40.0	38.0	41.0	35.0	41.0
20	38.84663147907344	40.0	38.0	41.0	35.0	41.0
21	38.80191740741228	40.0	38.0	41.0	35.0	41.0
22	38.74089792403495	40.0	38.0	41.0	35.0	41.0
23	38.69257122717072	40.0	38.0	41.0	34.0	41.0
24	38.638858294163256	40.0	38.0	41.0	34.0	41.0
25	38.58890196450689	40.0	38.0	41.0	34.0	41.0
26	38.46242283884031	40.0	38.0	41.0	34.0	41.0
27	38.34130088117352	40.0	38.0	41.0	34.0	41.0
28	38.25886589729952	40.0	38.0	41.0	34.0	41.0
29	38.186236415752575	40.0	38.0	41.0	33.0	41.0
30	38.106304471543424	40.0	38.0	41.0	33.0	41.0
31	38.073066866267915	40.0	38.0	41.0	33.0	41.0
32	37.982834807103345	40.0	37.0	41.0	33.0	41.0
33	37.91300495112237	40.0	37.0	41.0	33.0	41.0
34	37.85092538888861	40.0	37.0	41.0	33.0	41.0
35	37.794077184096274	40.0	37.0	41.0	33.0	41.0
36	37.763232704568196	40.0	37.0	41.0	33.0	41.0
37	37.63258366288584	40.0	37.0	41.0	32.0	41.0
38	37.48707625800339	40.0	37.0	41.0	32.0	41.0
39	37.43745966227359	40.0	37.0	41.0	32.0	41.0
40	37.266917773035026	40.0	36.0	41.0	31.0	41.0
41	37.202047669030115	39.0	36.0	41.0	31.0	41.0
42	37.06875297210412	39.0	36.0	41.0	31.0	41.0
43	36.1883629321218	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	1.0
11	5.0
12	3.0
13	2.0
14	1.0
15	4.0
16	10.0
17	24.0
18	46.0
19	108.0
20	240.0
21	507.0
22	950.0
23	1720.0
24	2811.0
25	4724.0
26	7181.0
27	10744.0
28	15534.0
29	22231.0
30	29304.0
31	38933.0
32	50448.0
33	65159.0
34	86600.0
35	117877.0
36	166861.0
37	262220.0
38	507421.0
39	810049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46208719262141	17.85737806254465	11.199092710572584	26.48144203426135
2	19.756754142096895	19.82788025559982	33.9828398031905	26.43252579911279
3	21.14998117383522	21.994819511809617	27.646347882998906	29.20885143135626
4	15.5413283142339	14.411031723790868	32.428602508126595	37.61903745384865
5	15.444676737264407	35.93708200350362	33.316952223326915	15.30128903590506
6	36.97654064566706	32.812847029349285	14.745179116537365	15.465433208446294
7	31.07311864389844	28.50490275116352	20.134957939758998	20.287020665179046
8	27.73804879178716	33.10979628227529	18.99103565707403	20.161119268863523
9	27.45295389111164	12.944225953946068	18.172949094867974	41.42987106007431
10	17.409637815474515	25.685974121176912	31.677372675854322	25.22701538749425
11	37.7606083962424	20.428954959365914	20.555446802345255	21.254989842046434
12	22.449872213716258	23.605830524548274	28.09658617364673	25.847711088088737
13	31.71561545208003	18.586261759540143	23.88679229857707	25.81133048980276
14	23.641802352066996	19.127337998467564	23.99902258367651	33.23183706578893
15	27.52794061741645	25.84430466502825	20.82246495131313	25.80528976624217
16	26.835664613577638	25.00096515320047	22.354310692126123	25.80905954109577
17	25.384165038017954	24.975984717423582	23.88415799807696	25.755692246481505
18	25.16642647599176	23.732095272656913	25.00319068293332	26.098287568418005
19	26.225642371906005	23.872439902748894	24.690662721877366	25.211255003467738
20	26.409997987939445	23.359341751891588	24.789721504476265	25.4409387556927
21	26.430709040147192	23.61518683322107	23.855680301291308	26.09842382534043
22	26.423078652491704	23.495416998414424	24.01714475435829	26.064359594735574
23	25.783761172499904	23.843190083402863	24.37836185569211	25.994686888405127
24	26.057864681433585	23.92308205891477	24.148768941415426	25.87028431823622
25	26.325291601168722	23.57526355495219	24.176565353588984	25.922879490290107
26	25.97356706543012	24.01319330360813	24.288704800740145	25.7245348302216
27	26.311711327900923	23.55464334069272	23.866944206877978	26.26670112452838
28	25.79974865139711	23.73754554955369	24.340664107156076	26.122041691893124
29	25.402241789725593	24.252596716299006	24.571074562967276	25.77408693100812
30	25.500755544634817	24.557176356880497	24.30655445757709	25.635513640907597
31	25.892585034538858	24.128875430742198	24.12324347794886	25.855296056770083
32	25.28783139386744	24.15244787832075	24.43431803183234	26.125402695979467
33	25.43026529676985	23.78064815601236	24.61063448944304	26.178452057774752
34	25.963665729067642	23.56840528985708	24.575752717303676	25.8921762637716
35	25.666443962296803	23.83038193269544	24.950595510879435	25.552578594128327
36	25.35972962993074	24.0106498410563	24.58397355162298	26.04564697738998
37	25.957034558843233	23.35448192165863	24.58165718394185	26.10682633555629
38	25.28601463490185	23.586845393357837	25.204169643501928	25.92297032823838
39	25.425814237304145	23.4240637900408	25.054832056530273	26.09528991612478
40	25.020313636184028	23.22962516174832	25.72049254152316	26.029568660544495
41	24.45507450301423	23.517172687027387	26.143116095893987	25.884636714064396
42	24.950186740112176	23.146145087279372	25.83767349480384	26.06599467780461
43	24.477556895213432	22.91682468684753	26.06395082396832	26.541667593970725
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	62.0
1	87.0
2	112.0
3	386.0
4	660.0
5	660.0
6	976.5
7	1293.0
8	1249.5
9	1206.0
10	1745.0
11	2284.0
12	2284.0
13	3831.0
14	5378.0
15	7775.0
16	10172.0
17	10196.0
18	10220.0
19	10220.0
20	11718.0
21	13216.0
22	13480.5
23	13745.0
24	16137.0
25	18529.0
26	18529.0
27	21574.0
28	24619.0
29	30698.5
30	36778.0
31	41660.0
32	46542.0
33	46542.0
34	51237.5
35	55933.0
36	61970.5
37	68008.0
38	76482.0
39	84956.0
40	84956.0
41	90819.5
42	96683.0
43	98522.0
44	100361.0
45	109203.5
46	118046.0
47	118046.0
48	126794.0
49	135542.0
50	146983.0
51	158424.0
52	172169.5
53	185915.0
54	185915.0
55	184876.5
56	183838.0
57	183777.5
58	183717.0
59	174689.0
60	165661.0
61	165661.0
62	155901.0
63	146141.0
64	130214.5
65	114288.0
66	98903.5
67	83519.0
68	83519.0
69	69566.0
70	55613.0
71	46114.0
72	36615.0
73	28628.5
74	20642.0
75	20642.0
76	15607.5
77	10573.0
78	8417.0
79	6261.0
80	4597.0
81	2933.0
82	2933.0
83	2195.5
84	1458.0
85	1179.0
86	900.0
87	739.5
88	579.0
89	579.0
90	421.0
91	263.0
92	143.5
93	24.0
94	18.0
95	12.0
96	12.0
97	6.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2201723.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.89354021925935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.16595540105114	54.41554396728709
2	9.174933227556078	11.724379303677464
3	2.4588873729036145	4.713210577657382
4	1.009352772996578	2.5796448798751124
5	0.5426672964485298	1.7336467365655441
6	0.32381680119718753	1.2413881086578646
7	0.23101988324283465	1.033247474099726
8	0.15667759539052725	0.8008548994033197
9	0.13298166310231557	0.7647002315866619
>10	0.6717655914666187	7.928853506286693
>50	0.0633630440238872	2.840609764192788
>100	0.06388702662101509	7.711201540626996
>500	0.004194653272508413	1.7331242590927622
>1k	4.976707272467151E-4	0.77959475099069
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4096	0.18603611807661544	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4018	0.18249343809371116	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3422	0.15542372950639113	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.54189741397987E-5	0.0	8.175415345163764E-4	0.0
3	0.0	4.54189741397987E-5	0.0	0.0012717312759143635	0.0
4	0.0	4.54189741397987E-5	0.0	0.002361786655269532	0.0
5	0.0	4.54189741397987E-5	0.0	0.00277055742252772	0.0
6	0.0	4.54189741397987E-5	0.0	0.002815976396667519	0.0
7	0.0	4.54189741397987E-5	0.0	0.0032247471639257072	0.0
8	0.0	4.54189741397987E-5	0.0	0.0034972610087644993	0.0
9	0.0	4.54189741397987E-5	0.0	0.005768209715754434	0.0
10	0.0	4.54189741397987E-5	0.0	0.009356308672798531	0.0
11	0.0	4.54189741397987E-5	0.0	0.01003759328489551	0.0
12	0.0	4.54189741397987E-5	0.0	0.01108222969011088	0.0
13	0.0	4.54189741397987E-5	0.0	0.011718095328068064	0.0
14	0.0	4.54189741397987E-5	0.0	0.012353960966025245	0.0
15	0.0	4.54189741397987E-5	0.0	0.013080664552262023	0.0
16	0.0	4.54189741397987E-5	0.0	0.014079881983337594	0.0
17	0.0	4.54189741397987E-5	0.0	0.016032897871348938	0.0
18	0.0	4.54189741397987E-5	0.0	0.017168372224843905	0.0
19	0.0	4.54189741397987E-5	0.0	0.01848552247489807	0.0
20	0.0	4.54189741397987E-5	0.0	0.020393119388769615	0.0
21	0.0	4.54189741397987E-5	0.0	0.023027419888877937	0.0
22	0.0	4.54189741397987E-5	0.0	0.027750993199417002	0.0
23	0.0	4.54189741397987E-5	0.0	0.03383713573415003	0.0
24	0.0	4.54189741397987E-5	0.0	0.04355679620006695	0.0
25	0.0	4.54189741397987E-5	0.0	0.046509029519153866	0.0
26	0.0	4.54189741397987E-5	0.0	0.051323440777972525	0.0
27	0.0	4.54189741397987E-5	4.54189741397987E-5	0.05849963869206072	0.0
28	0.0	4.54189741397987E-5	4.54189741397987E-5	0.07852940628771195	0.0
29	0.0	4.54189741397987E-5	4.54189741397987E-5	0.10814257742686069	0.0
30	0.0	4.54189741397987E-5	4.54189741397987E-5	0.15210814439418582	0.0
31	0.0	4.54189741397987E-5	4.54189741397987E-5	0.3205671194786992	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2305	0.0	16.3731	1
TAATAGT	210	9.094947E-12	14.97619	4
TAGGACA	570	0.0	14.929824	4
GTATTAG	275	0.0	14.8	1
TTAGGAC	565	0.0	14.734513	3
GTGTAGG	375	0.0	14.306666	1
GTTAGAC	130	4.4481258E-6	14.230769	3
TAACACA	275	0.0	14.127274	4
GGTATAC	145	8.9198693E-7	14.034483	1
GGCGTAT	80	0.006301035	13.875001	1
TAACCCG	240	5.456968E-12	13.875001	28
ACGCTAA	80	0.006301035	13.875001	34
GCTTAGG	535	0.0	13.831776	1
TCTTATA	1475	0.0	13.6711855	37
CTTAGGA	655	0.0	13.274809	2
CTTATAC	950	0.0	13.2421055	37
TACACTA	130	7.003491E-5	12.807693	5
GTCTAGA	190	1.0471558E-7	12.657895	1
TATACAA	615	0.0	12.634146	5
TTCTATA	220	4.089088E-9	12.613636	2
>>END_MODULE