FastQCFastQC Report
Fri 10 Feb 2017
ERR1630642.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630642.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699781
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72730.42787865024964983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63490.37351870623333244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52520.3089809804910162No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36420.21426289622016012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTTA555.144488E-420.18181819
AACCGCG1352.2213499E-816.4444457
GGTATCA35700.015.9607831
CCGACCA2150.015.4883729
TAATACT3500.015.3285714
GGTCTAC1455.3498297E-815.3103451
CGACCAT1954.1836756E-1115.17948710
ATGTAGG1853.0559022E-1015.01
GTATTAG2500.014.7999991
GAACCGC1551.211065E-714.3225816
TCTTATA14900.014.15436237
TCTAATA2750.014.1272742
CAAGACG3050.013.950824
TATGCGG800.006300256613.8750015
AATAACG1753.5684934E-813.7428572
CGCAATT1356.5709246E-613.70370412
GCGGGTA2355.638867E-1113.382977523
GCGTTAT1805.162474E-813.3611111
CTTATAC9700.013.35051537
CTAGCGG1254.8470494E-513.3229
Produced by FastQC (version 0.11.5)