##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630642.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1699781
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.704778439104803	31.0	31.0	34.0	30.0	34.0
2	31.88152179604314	33.0	31.0	34.0	30.0	34.0
3	31.976083389566067	33.0	31.0	34.0	30.0	34.0
4	35.638979374401764	37.0	35.0	37.0	33.0	37.0
5	35.55511798284603	37.0	35.0	37.0	33.0	37.0
6	35.62451103995162	37.0	35.0	37.0	33.0	37.0
7	35.57784855813778	37.0	35.0	37.0	33.0	37.0
8	35.58221206143615	37.0	35.0	37.0	33.0	37.0
9	37.22584909467749	39.0	37.0	39.0	34.0	39.0
10	37.07064086491142	39.0	37.0	39.0	33.0	39.0
11	37.19774488595884	39.0	37.0	39.0	34.0	39.0
12	37.13037914884329	39.0	37.0	39.0	33.0	39.0
13	37.190909887803194	39.0	37.0	39.0	34.0	39.0
14	38.39337008708769	40.0	38.0	41.0	34.0	41.0
15	38.39811481596747	40.0	38.0	41.0	34.0	41.0
16	38.34033266638467	40.0	38.0	41.0	34.0	41.0
17	38.313216820284495	40.0	38.0	41.0	34.0	41.0
18	38.29781012965788	40.0	38.0	41.0	34.0	41.0
19	38.36037348340757	40.0	38.0	41.0	34.0	41.0
20	38.322220333089966	40.0	38.0	41.0	34.0	41.0
21	38.2794259966431	40.0	38.0	41.0	34.0	41.0
22	38.205597073976	40.0	38.0	41.0	34.0	41.0
23	38.14132055835428	40.0	38.0	41.0	33.0	41.0
24	38.078929579751744	40.0	37.0	41.0	33.0	41.0
25	38.025793911097956	40.0	37.0	41.0	33.0	41.0
26	37.86659340232654	40.0	37.0	41.0	33.0	41.0
27	37.71924736186603	40.0	37.0	41.0	33.0	41.0
28	37.61289130776259	40.0	37.0	41.0	32.0	41.0
29	37.52256849558855	40.0	37.0	41.0	32.0	41.0
30	37.41968288856035	39.0	36.0	41.0	32.0	41.0
31	37.374847112657456	39.0	36.0	41.0	32.0	41.0
32	37.267100879466234	39.0	36.0	41.0	31.0	41.0
33	37.1812174627202	39.0	36.0	41.0	31.0	41.0
34	37.10667609533228	39.0	36.0	41.0	31.0	41.0
35	37.043435007215635	39.0	36.0	40.0	31.0	41.0
36	37.00973890165851	39.0	36.0	40.0	31.0	41.0
37	36.8621887172524	39.0	35.0	40.0	31.0	41.0
38	36.706691038433775	39.0	35.0	40.0	30.0	41.0
39	36.63437760511501	39.0	35.0	40.0	30.0	41.0
40	36.45000973654842	39.0	35.0	40.0	30.0	41.0
41	36.37290686270761	39.0	35.0	40.0	30.0	41.0
42	36.21917882362492	39.0	35.0	40.0	30.0	41.0
43	35.394510822276516	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	3.0
11	6.0
12	2.0
13	3.0
14	1.0
15	8.0
16	11.0
17	51.0
18	82.0
19	174.0
20	354.0
21	708.0
22	1175.0
23	2110.0
24	3383.0
25	5456.0
26	8160.0
27	11877.0
28	16512.0
29	23071.0
30	30317.0
31	39195.0
32	49775.0
33	63681.0
34	83485.0
35	111725.0
36	158161.0
37	249370.0
38	453121.0
39	387797.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.970535616058775	19.867853564665094	12.513494385453185	24.648116433822945
2	18.98221006117847	21.627197856665063	34.333599446046286	25.05699263611018
3	20.86445253829758	23.76100215263025	29.373784034531507	26.00076127454066
4	14.957515115182485	16.01341584592368	34.09256839557567	34.93650064331817
5	14.391854009428274	37.25362267256782	34.02844248759105	14.32608083041286
6	34.764478482816315	34.47073475936018	15.777797257411397	14.986989500412113
7	28.67622358409701	30.75202040733483	21.638493429447674	18.933262579120484
8	27.30245837552014	33.312291406951836	20.191542322216804	19.193707895311217
9	26.73667960754944	13.810661491097969	20.367270842537952	39.08538805881464
10	17.131618720293968	27.11766986452961	33.11632498539518	22.634386429781248
11	34.82130933337883	22.131203960980855	21.589134129631994	21.458352576008323
12	21.30950987215412	25.943871592869904	29.484445349136152	23.262173185839824
13	29.896733755701472	20.30838090318694	25.58370754820768	24.21117779290391
14	22.77769900946063	21.51700719092636	25.944695228385307	29.760598571227703
15	25.591002605629786	27.69592082744777	23.078561297014144	23.6345152699083
16	24.674237445882735	26.748328166981512	24.901855003674005	23.675579383461752
17	22.996550732123726	26.983064288870157	26.36969115433106	23.650693824675063
18	22.98719658591313	25.461574167495694	27.7530458335515	23.79818341303968
19	23.611865293234835	25.83238664274986	27.83423276292652	22.721515301088786
20	24.096045314072814	25.062463929176758	27.649444251935982	23.192046504814442
21	24.36484464763402	25.46039754533084	26.918055914261895	23.256701892773247
22	24.16228914195417	25.531759679629317	26.98382909327731	23.322122085139203
23	23.705171430907864	25.71943091492375	27.147379574192204	23.428018079976184
24	23.60362893808085	26.02782358433233	27.043895654793175	23.324651822793644
25	23.862956463214967	25.670071615108064	26.982887795545423	23.484084126131542
26	23.432077426444938	26.412108383374093	26.96959196508256	23.186222225098412
27	23.879664497955915	25.77267306788345	26.611251684775862	23.736410749384774
28	23.351243483719372	25.957520409982227	27.31398927273572	23.377246833562676
29	22.9654290758633	26.225966756893975	27.551431625603534	23.257172541639186
30	23.05385223155218	26.605133249518616	27.408766188114818	22.93224833081438
31	23.352184781451257	26.506532312103737	26.946941988409094	23.19434091803591
32	22.854061787959743	26.299270317764467	27.140849321177257	23.705818573098533
33	22.810762092293064	26.217200921765805	27.505013881199993	23.46702310474114
34	23.555093273780564	25.767907748115785	27.156557227078075	23.520441751025572
35	23.207166099632833	26.004997114334138	27.728336768089534	23.05950001794349
36	22.886124741952052	26.242262973877224	27.181030968107066	23.690581316063657
37	23.286235109111114	25.548232389937294	27.485305459938665	23.68022704101293
38	22.897949794708847	25.4867538818236	27.945717713046562	23.669578610420988
39	22.909245367491458	25.562763673673256	27.91800826106422	23.609982697771066
40	22.43030131528709	25.453455474558197	28.481492615813448	23.634750594341273
41	21.987714887976743	25.396330468454465	28.816771101688982	23.799183541879806
42	22.315286498672478	25.449984439171867	28.535028924314364	23.699700137841287
43	21.978772559523847	25.091938314406388	28.69346109881214	24.23582802725763
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	318.0
1	296.0
2	274.0
3	679.0
4	1084.0
5	1084.0
6	1532.5
7	1981.0
8	2044.0
9	2107.0
10	3081.5
11	4056.0
12	4056.0
13	7110.0
14	10164.0
15	13891.0
16	17618.0
17	17488.5
18	17359.0
19	17359.0
20	20437.0
21	23515.0
22	22647.5
23	21780.0
24	24715.5
25	27651.0
26	27651.0
27	31552.5
28	35454.0
29	40405.0
30	45356.0
31	51392.0
32	57428.0
33	57428.0
34	62822.5
35	68217.0
36	73732.0
37	79247.0
38	84669.0
39	90091.0
40	90091.0
41	93443.0
42	96795.0
43	97813.5
44	98832.0
45	101147.0
46	103462.0
47	103462.0
48	105334.5
49	107207.0
50	110226.0
51	113245.0
52	115768.0
53	118291.0
54	118291.0
55	114171.0
56	110051.0
57	105486.0
58	100921.0
59	93521.0
60	86121.0
61	86121.0
62	80618.5
63	75116.0
64	66460.5
65	57805.0
66	50035.0
67	42265.0
68	42265.0
69	36403.5
70	30542.0
71	26264.0
72	21986.0
73	17955.5
74	13925.0
75	13925.0
76	10977.0
77	8029.0
78	6547.0
79	5065.0
80	3980.0
81	2895.0
82	2895.0
83	2213.5
84	1532.0
85	1253.5
86	975.0
87	814.0
88	653.0
89	653.0
90	474.5
91	296.0
92	177.5
93	59.0
94	33.5
95	8.0
96	8.0
97	6.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1699781.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.73645711544067
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.31869621279351	55.87966575640259
2	8.744946075135688	11.322336531397255
3	2.09060985006254	4.060160247110744
4	0.8535610700416937	2.2102607842465503
5	0.4521816815179154	1.463632001698619
6	0.26630802293214517	1.0343902743626763
7	0.19228480057250977	0.8713485722349338
8	0.13508374045878505	0.6995874216962765
9	0.10699277849612653	0.6233700075098704
>10	0.6944917803138053	8.544247173675048
>50	0.08041830824411669	3.601861726122664
>100	0.061514022685165086	7.285710932508777
>500	0.0024567103793520987	1.0171410083723582
>1k	1.81978546618674E-4	0.2745479638651588
>5k	2.72967819928011E-4	1.1117395987966117
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7273	0.42787865024964983	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6349	0.37351870623333244	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5252	0.3089809804910162	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3642	0.21426289622016012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.7649332472830323E-4	0.0
2	0.0	0.0	0.0	0.0011177910566125872	0.0
3	0.0	0.0	0.0	0.0015296088143119614	0.0
4	0.0	0.0	0.0	0.002294413221467942	0.0
5	0.0	0.0	0.0	0.0024120754379534774	0.0
6	0.0	0.0	0.0	0.0024709065461962455	0.0
7	0.0	0.0	0.0	0.002588568762681781	0.0
8	0.0	0.0	0.0	0.002765062087410084	0.0
9	0.0	0.0	0.0	0.004706488659421419	0.0
10	0.0	5.883110824276774E-5	0.0	0.007353888530345968	0.0
11	0.0	5.883110824276774E-5	0.0	0.00835401737047302	0.0
12	0.0	5.883110824276774E-5	0.0	0.009118821777629	0.0
13	0.0	5.883110824276774E-5	0.0	0.009824795076542213	0.0
14	0.0	5.883110824276774E-5	0.0	0.010530768375455427	0.0
15	0.0	5.883110824276774E-5	0.0	0.011648559432068013	0.0
16	0.0	5.883110824276774E-5	0.0	0.014060634870021491	0.0
17	0.0	1.7649332472830323E-4	0.0	0.016002061442032825	0.0
18	0.0	1.7649332472830323E-4	0.0	0.01694335917391711	0.0
19	0.0	1.7649332472830323E-4	0.0	0.019061279070656748	0.0
20	0.0	1.7649332472830323E-4	0.0	0.02041439456024041	0.0
21	0.0	1.7649332472830323E-4	0.0	0.023944261054806473	0.0
22	0.0	1.7649332472830323E-4	0.0	0.031121656260424138	0.0
23	0.0	2.941555412138387E-4	0.0	0.03982866028035376	0.0
24	0.0	2.941555412138387E-4	0.0	0.053124490743219274	0.0
25	0.0	2.941555412138387E-4	0.0	0.05771331718615516	0.0
26	0.0	2.941555412138387E-4	0.0	0.06412590798461684	0.0
27	0.0	2.941555412138387E-4	0.0	0.07865719172058047	0.0
28	0.0	2.941555412138387E-4	0.0	0.10913170579033417	0.0
29	0.0	2.941555412138387E-4	0.0	0.1510194548591848	0.0
30	0.0	2.941555412138387E-4	0.0	0.2152041939520444	0.0
31	0.0	2.941555412138387E-4	0.0	0.42340748602319944	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGTTA	55	5.144488E-4	20.181818	19
AACCGCG	135	2.2213499E-8	16.444445	7
GGTATCA	3570	0.0	15.960783	1
CCGACCA	215	0.0	15.488372	9
TAATACT	350	0.0	15.328571	4
GGTCTAC	145	5.3498297E-8	15.310345	1
CGACCAT	195	4.1836756E-11	15.179487	10
ATGTAGG	185	3.0559022E-10	15.0	1
GTATTAG	250	0.0	14.799999	1
GAACCGC	155	1.211065E-7	14.322581	6
TCTTATA	1490	0.0	14.154362	37
TCTAATA	275	0.0	14.127274	2
CAAGACG	305	0.0	13.95082	4
TATGCGG	80	0.0063002566	13.875001	5
AATAACG	175	3.5684934E-8	13.742857	2
CGCAATT	135	6.5709246E-6	13.703704	12
GCGGGTA	235	5.638867E-11	13.3829775	23
GCGTTAT	180	5.162474E-8	13.361111	1
CTTATAC	970	0.0	13.350515	37
CTAGCGG	125	4.8470494E-5	13.32	29
>>END_MODULE