FastQCFastQC Report
Fri 10 Feb 2017
ERR1630641.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630641.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2266734
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT95820.4227227367657608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT81680.36034223689237466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT64160.2830504152670759No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42050.1855091951680259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG2000.018.528
TGCGTAT609.240591E-418.512
CGCAATA2150.018.06976736
AGTCGGT4000.017.11250111
CGAACGA1757.2759576E-1216.91428616
GTATAGA2350.016.5319161
TATACTG2350.016.5319165
TATCCCG803.3843805E-416.18755
CAGTCGG4350.016.16092110
CGAATTA1751.3278623E-1015.85714315
AAGACCG1557.219569E-915.51612955
CTTATAC16450.015.40729537
GCAGTCG4850.015.2577329
GTACCGT855.367008E-415.2352946
TACCGTT750.004106043414.87
GGTATCA43800.014.4029681
TATAGTG2700.014.3888885
ATCTCGC4000.014.33750111
ATAAGAC1551.2116652E-714.3225813
TCTTATA25200.014.24206437
Produced by FastQC (version 0.11.5)