FastQCFastQC Report
Fri 10 Feb 2017
ERR1630640.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630640.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368826
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT96130.702280640490464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT85500.6246228519914145No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77930.5693199866162683No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55790.40757554283743885No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG22230.16240194151776777No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT18800.13734397213378471No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG17990.13142649248333974No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16070.11739987405265534No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15230.11126322848923091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACGC250.005496705429.63
CGAACGT555.143784E-420.1818184
ACGAACG1153.0522642E-919.30434615
AACGTCT701.21941324E-418.56
CGTTATA500.007035551618.52
CGAACGA1258.581992E-917.76000216
GACGGAC1107.810286E-716.8181827
GTCTAAT904.446953E-516.4444451
TTAGAAC803.3831564E-416.18753
GTTATAC803.3831564E-416.18753
GACTTAC700.002592832815.8571421
GTACTAT1059.345626E-615.8571421
TAACGAA1557.210474E-915.51612913
CGAGCCG1557.210474E-915.51612915
TTAGGAC8250.015.4727283
GCTTAGG7200.015.4166671
TCCGATA1455.347283E-815.3103458
ACGGAAT1353.973455E-715.07407518
ATACCGC1601.0966687E-815.0312527
CTTAGGA7700.014.8961042
Produced by FastQC (version 0.11.5)