##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630640.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1368826
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.945078483313438	33.0	31.0	34.0	30.0	34.0
2	32.10483143949633	33.0	31.0	34.0	30.0	34.0
3	32.184165116676624	34.0	31.0	34.0	30.0	34.0
4	35.80411315974419	37.0	35.0	37.0	35.0	37.0
5	35.75674483097194	37.0	35.0	37.0	35.0	37.0
6	35.843228430786674	37.0	35.0	37.0	35.0	37.0
7	35.78652217301541	37.0	35.0	37.0	35.0	37.0
8	35.802231255104736	37.0	35.0	37.0	35.0	37.0
9	37.47058282060686	39.0	37.0	39.0	35.0	39.0
10	37.37963042782647	39.0	37.0	39.0	34.0	39.0
11	37.479537209258154	39.0	37.0	39.0	35.0	39.0
12	37.43484489628339	39.0	37.0	39.0	35.0	39.0
13	37.46455137468166	39.0	37.0	39.0	35.0	39.0
14	38.733018659785834	40.0	38.0	41.0	35.0	41.0
15	38.73114406067681	40.0	38.0	41.0	35.0	41.0
16	38.67585434525645	40.0	38.0	41.0	34.0	41.0
17	38.68707271778882	40.0	38.0	41.0	35.0	41.0
18	38.67261142029739	40.0	38.0	41.0	35.0	41.0
19	38.7616578001879	40.0	38.0	41.0	35.0	41.0
20	38.72960843817987	40.0	38.0	41.0	35.0	41.0
21	38.68452966264522	40.0	38.0	41.0	34.0	41.0
22	38.620696859936906	40.0	38.0	41.0	34.0	41.0
23	38.55559070327419	40.0	38.0	41.0	34.0	41.0
24	38.49135609639209	40.0	38.0	41.0	34.0	41.0
25	38.43819594309284	40.0	38.0	41.0	34.0	41.0
26	38.2662946203535	40.0	38.0	41.0	34.0	41.0
27	38.11953308893899	40.0	37.0	41.0	33.0	41.0
28	38.0148002741035	40.0	37.0	41.0	33.0	41.0
29	37.92647129730148	40.0	37.0	41.0	33.0	41.0
30	37.81336342237801	40.0	37.0	41.0	33.0	41.0
31	37.72829709546721	40.0	37.0	41.0	33.0	41.0
32	37.5870132507711	40.0	37.0	41.0	33.0	41.0
33	37.4862517222788	40.0	36.0	41.0	33.0	41.0
34	37.391648755941226	40.0	36.0	41.0	32.0	41.0
35	37.29659284671682	40.0	36.0	41.0	32.0	41.0
36	37.21933832349765	40.0	36.0	41.0	32.0	41.0
37	37.045235113885916	40.0	36.0	41.0	31.0	41.0
38	36.84664888013524	39.0	35.0	41.0	31.0	41.0
39	36.73030392467706	39.0	35.0	41.0	31.0	41.0
40	36.51227109946772	39.0	35.0	41.0	30.0	41.0
41	36.39012043897471	39.0	35.0	41.0	30.0	41.0
42	36.20496396181838	39.0	35.0	41.0	29.0	41.0
43	35.40351366791688	38.0	35.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	5.0
11	3.0
12	4.0
13	2.0
14	5.0
15	4.0
16	14.0
17	19.0
18	48.0
19	130.0
20	232.0
21	472.0
22	815.0
23	1499.0
24	2396.0
25	3925.0
26	5901.0
27	8630.0
28	12156.0
29	16906.0
30	22490.0
31	28446.0
32	36366.0
33	46284.0
34	60918.0
35	79567.0
36	111422.0
37	177404.0
38	317769.0
39	434989.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.73563477023376	19.377773361990496	12.23939346564136	23.647198402134386
2	18.253160007188644	22.03618283112682	35.15720770938016	24.55344945230438
3	21.315638364554733	24.982722420526787	29.260475765363896	24.44116344955458
4	14.903428193210825	15.772274927565666	33.44807886466213	35.87621801456138
5	13.550736178301698	38.93504360671115	33.574756762364245	13.939463452622904
6	34.276891292246056	33.578263416971915	16.40120804251234	15.743637248269685
7	27.719593286509753	31.698112104825594	21.991034653053056	18.591259955611598
8	27.113745647730248	33.57636397905942	20.5284674604369	18.781422912773426
9	26.86667260849809	13.947572591403143	20.659163399876977	38.5265914002218
10	17.12219084091039	28.224478494710066	32.4774660913805	22.175864572999053
11	33.352595581907416	22.82634900272204	21.85595539535339	21.965100020017154
12	21.10750380252859	27.110677324948533	29.2792509785758	22.502567893947077
13	29.084996924371687	20.765458867672002	27.059757777832978	23.089786430123333
14	22.87514994601213	22.023398152869685	26.608787384225607	28.492664516892578
15	25.536919959147475	27.64493076548809	24.551696125000547	22.266453150363887
16	24.133819784253074	27.022280406713488	25.82000926341259	23.023890545620844
17	22.619456380869448	27.48201743684004	26.88362143910183	23.014904743188687
18	21.50426716032571	25.514930312545204	29.32564109682312	23.65516143030597
19	22.91379620200084	26.423299966540668	29.395847244280866	21.267056587177624
20	23.407211727421892	24.96292443305431	29.78413618677611	21.845727652747684
21	23.2731552439828	26.092286382637386	28.268676953827587	22.36588141955223
22	23.33693252465982	25.619910784862356	28.28540661851835	22.757750071959475
23	22.465674965262203	26.432358824277152	28.547309884528786	22.55465632593186
24	23.01285919466755	26.82956051390023	28.355393600063124	21.802186691369098
25	22.966103799898598	25.963489881109798	28.598813874078953	22.47159244491265
26	22.81597514950768	26.598267420402593	28.377821578491346	22.207935851598375
27	22.939438613819433	26.096815811505625	28.09831198413823	22.865433590536707
28	22.06920382868239	26.439737410014132	28.963432897972424	22.527625863331057
29	21.93003347394044	27.11009288251392	28.82236310531799	22.13751053822765
30	21.595586290733813	27.78965332335885	28.704744065352354	21.910016320554988
31	22.284351699923878	27.22968441569637	28.477395958288344	22.00856792609141
32	21.38927811131583	27.254888495689006	28.645277047630596	22.71055634536457
33	21.738409410692082	26.90371164779161	28.73988366673339	22.617995274782913
34	22.099010392847592	27.036745356970133	28.33398839589546	22.530255854286814
35	22.21136945090172	27.118128965989836	28.47600790750614	22.194493675602303
36	21.847554035355845	27.35139455270429	28.248148413311846	22.55290299862802
37	22.593813969050853	26.360034072993937	28.15712150412105	22.88903045383416
38	21.925430989767875	26.67913964229201	28.831348907750144	22.564080460189974
39	21.978542196013226	26.684326568899188	28.757855271597705	22.57927596348988
40	21.580171621520925	26.210635975646284	29.397892792802004	22.811299610030787
41	20.972862876654887	26.88486337927538	29.45947841434923	22.682795329720502
42	21.75338574807901	26.111938259501205	29.13072954488006	23.003946447539718
43	20.994633357344178	26.300274834054875	29.076084177243857	23.62900763135709
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	205.0
1	239.5
2	274.0
3	873.5
4	1473.0
5	1473.0
6	2182.0
7	2891.0
8	3060.0
9	3229.0
10	4775.5
11	6322.0
12	6322.0
13	10536.0
14	14750.0
15	19966.0
16	25182.0
17	24065.0
18	22948.0
19	22948.0
20	24542.0
21	26136.0
22	23448.5
23	20761.0
24	21709.5
25	22658.0
26	22658.0
27	24775.5
28	26893.0
29	32998.5
30	39104.0
31	41530.0
32	43956.0
33	43956.0
34	47463.0
35	50970.0
36	53545.5
37	56121.0
38	64482.0
39	72843.0
40	72843.0
41	77651.0
42	82459.0
43	79223.0
44	75987.0
45	81303.5
46	86620.0
47	86620.0
48	91047.0
49	95474.0
50	97319.0
51	99164.0
52	101556.0
53	103948.0
54	103948.0
55	92011.5
56	80075.0
57	75289.5
58	70504.0
59	63835.5
60	57167.0
61	57167.0
62	53507.0
63	49847.0
64	44705.5
65	39564.0
66	34809.5
67	30055.0
68	30055.0
69	25689.0
70	21323.0
71	18646.0
72	15969.0
73	12903.0
74	9837.0
75	9837.0
76	7764.5
77	5692.0
78	4723.5
79	3755.0
80	2937.0
81	2119.0
82	2119.0
83	1603.5
84	1088.0
85	907.0
86	726.0
87	594.0
88	462.0
89	462.0
90	344.0
91	226.0
92	129.5
93	33.0
94	22.5
95	12.0
96	12.0
97	8.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1368826.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.81242406426253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.28597897627645	45.56971712495154
2	7.912878435678948	8.3579658302807
3	2.1713509551261514	3.4402292230339135
4	0.9637722955815856	2.035966047025699
5	0.5394595445970328	1.4245083117386224
6	0.3576153178594025	1.1331919091200082
7	0.2292810522465521	0.847622171280167
8	0.1974906362946048	0.8343967386169364
9	0.13241439655384987	0.6293812736713807
>10	0.9295440752335377	9.68183228422837
>50	0.1337791363597919	5.080712729527798
>100	0.13452087736444876	13.863620420166981
>500	0.00886645642993538	3.0433180426246316
>1k	0.0024936908709193256	1.7502645562126804
>5k	5.541535268709613E-4	2.3072733375205616
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9613	0.702280640490464	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8550	0.6246228519914145	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7793	0.5693199866162683	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5579	0.40757554283743885	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2223	0.16240194151776777	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1880	0.13734397213378471	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1799	0.13142649248333974	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1607	0.11739987405265534	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1523	0.11126322848923091	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.305530432648124E-5	0.0
2	0.0	0.0	0.0	0.0014611060865296246	0.0
3	0.0	0.0	0.0	0.001899437912488512	0.0
4	0.0	0.0	0.0	0.0027030462600798055	0.0
5	0.0	0.0	0.0	0.003141378086038693	0.0
6	0.0	0.0	0.0	0.0032144333903651743	0.0
7	0.0	0.0	0.0	0.0035066546076710993	0.0
8	0.0	0.0	0.0	0.003944986433629986	0.0
9	0.0	0.0	0.0	0.0071594198239951605	0.0
10	0.0	1.4611060865296247E-4	0.0	0.01073912973599274	0.0
11	0.0	1.4611060865296247E-4	0.0	0.012638567648481253	0.0
12	0.0	2.191659129794437E-4	0.0	0.014464950256643284	0.0
13	0.0	2.191659129794437E-4	0.0	0.01607216695182587	0.0
14	0.0	2.191659129794437E-4	0.0	0.017898549559987903	0.0
15	0.0	2.191659129794437E-4	0.0	0.021405204167659002	0.0
16	0.0	2.191659129794437E-4	0.0	0.02849156868732768	0.0
17	0.0	2.191659129794437E-4	0.0	0.038207924162749686	0.0
18	0.0	2.191659129794437E-4	0.0	0.04149541285744134	0.0
19	0.0	2.191659129794437E-4	0.0	0.04902010920306891	0.0
20	0.0	2.191659129794437E-4	0.0	0.05515675476649333	0.0
21	0.0	2.9222121730592494E-4	0.0	0.0677222671106481	0.0
22	0.0	2.9222121730592494E-4	0.0	0.08846997353936878	0.0
23	0.0	2.9222121730592494E-4	0.0	0.11739987405265534	0.0
24	0.0	3.6527652163240615E-4	0.0	0.16335166047401203	0.0
25	0.0	3.6527652163240615E-4	0.0	0.1748213432532696	0.0
26	0.0	3.6527652163240615E-4	0.0	0.19410794359546063	0.0
27	0.0	3.6527652163240615E-4	0.0	0.2182892493275259	0.0
28	0.0	3.6527652163240615E-4	0.0	0.2533557954042369	0.0
29	0.0	3.6527652163240615E-4	0.0	0.30222979399865285	0.0
30	0.0	3.6527652163240615E-4	0.0	0.37426232406456333	0.0
31	0.0	3.6527652163240615E-4	0.0	0.6024140394761642	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTACGC	25	0.0054967054	29.6	3
CGAACGT	55	5.143784E-4	20.181818	4
ACGAACG	115	3.0522642E-9	19.304346	15
AACGTCT	70	1.21941324E-4	18.5	6
CGTTATA	50	0.0070355516	18.5	2
CGAACGA	125	8.581992E-9	17.760002	16
GACGGAC	110	7.810286E-7	16.818182	7
GTCTAAT	90	4.446953E-5	16.444445	1
TTAGAAC	80	3.3831564E-4	16.1875	3
GTTATAC	80	3.3831564E-4	16.1875	3
GACTTAC	70	0.0025928328	15.857142	1
GTACTAT	105	9.345626E-6	15.857142	1
TAACGAA	155	7.210474E-9	15.516129	13
CGAGCCG	155	7.210474E-9	15.516129	15
TTAGGAC	825	0.0	15.472728	3
GCTTAGG	720	0.0	15.416667	1
TCCGATA	145	5.347283E-8	15.310345	8
ACGGAAT	135	3.973455E-7	15.074075	18
ATACCGC	160	1.0966687E-8	15.03125	27
CTTAGGA	770	0.0	14.896104	2
>>END_MODULE