FastQCFastQC Report
Fri 10 Feb 2017
ERR1630639.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630639.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334992
Sequences flagged as poor quality0
Sequence length43
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT128473.8350169556287907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT115533.448739074365955No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80012.3884152457372116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66711.9913908391842192No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT23480.7009122605912977No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18320.5468787314323924No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.5038926302717677No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT15200.4537421789177055TruSeq Adapter, Index 3 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15160.4525481205521326No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG11780.3516501886612218No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11520.3438888092849978No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10460.31224626259731575No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT9870.29463390170511533No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG9160.2734393657161962No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC9150.27314085112480296No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC7980.2382146439317954No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.22985623537278502No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA7640.22806514782442566No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA7590.22657257486745952No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG7280.21731862253426948No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA6760.20179586378182165No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6500.19403448440559776No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6380.19045230930887902No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5840.17433252137364474No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5580.16657114199742082No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA5210.15552610211587142No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5010.14955581028800688No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4940.14746620814825429No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC4910.1465706643740746No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA4530.13522710990113196No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4530.13522710990113196No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4470.1334360223527726No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA4350.12985384725605387No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC3710.11074891340688733No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG3580.10686822371877537No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA3560.10627119453598892No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA3470.10358456321344987No Hit
GAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA3350.10000238811673115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAC200.001840074537.016
ACTATAT250.00549085429.5999983
ATCCCTA250.00549085429.59999822
ACCAGCG250.00549085429.59999812
CGTCTTC706.557457E-926.4285737
TACACTC358.856225E-426.428573
CCGTCTT2350.025.97872237
GCCGTCT2600.023.4807736
ATGCCCC652.6734324E-622.7692337
TATTGAC603.7161473E-521.58333430
TGTTTAT450.00382023620.55555535
TTGTTTA450.00382023620.55555534
AGAGGCG450.00382023620.5555555
ATTGACT656.883486E-519.92307731
TATACAC6050.019.2644623
TGGGCAG4400.018.9204546
TGCCGTC3150.018.79365235
GCAGTCG4150.018.722899
CAGTCGG4200.018.50000210
AGTCGGT4200.018.50000211
Produced by FastQC (version 0.11.5)