##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630639.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	334992
Sequences flagged as poor quality	0
Sequence length	43
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.992961025934946	33.0	31.0	34.0	30.0	34.0
2	32.018561637292834	33.0	31.0	34.0	30.0	34.0
3	31.999101471079907	33.0	31.0	34.0	30.0	34.0
4	35.68051177341548	37.0	35.0	37.0	33.0	37.0
5	35.698619668529396	37.0	35.0	37.0	35.0	37.0
6	35.865629626976165	37.0	35.0	37.0	35.0	37.0
7	35.75080300425085	37.0	35.0	37.0	35.0	37.0
8	35.873334288580025	37.0	35.0	37.0	35.0	37.0
9	37.403600683001386	39.0	37.0	39.0	34.0	39.0
10	37.37323876391078	39.0	37.0	39.0	34.0	39.0
11	37.54384582318384	39.0	37.0	39.0	35.0	39.0
12	37.54815637388356	39.0	37.0	39.0	35.0	39.0
13	37.613101208387064	39.0	37.0	39.0	35.0	39.0
14	38.67136230118928	40.0	38.0	41.0	34.0	41.0
15	38.64580049672828	40.0	38.0	41.0	34.0	41.0
16	38.4694470315709	40.0	38.0	41.0	34.0	41.0
17	38.69019558676028	40.0	38.0	41.0	35.0	41.0
18	38.8006519558676	40.0	38.0	41.0	35.0	41.0
19	38.93468500740316	40.0	38.0	41.0	35.0	41.0
20	38.93539547213068	40.0	38.0	41.0	35.0	41.0
21	38.90414397955772	40.0	38.0	41.0	35.0	41.0
22	38.73999677604241	40.0	38.0	41.0	35.0	41.0
23	38.61362957921383	40.0	38.0	41.0	35.0	41.0
24	38.47340832019869	40.0	38.0	41.0	35.0	41.0
25	38.34081410899365	40.0	37.0	41.0	34.0	41.0
26	37.96662606868224	40.0	37.0	41.0	34.0	41.0
27	37.624238787791946	40.0	35.0	41.0	33.0	41.0
28	37.3911765057076	39.0	35.0	41.0	33.0	41.0
29	37.12405967903711	39.0	35.0	41.0	33.0	41.0
30	36.745835721450064	38.0	35.0	41.0	33.0	41.0
31	36.33657818694178	38.0	35.0	40.0	32.0	41.0
32	35.922138439126904	37.0	35.0	40.0	31.0	41.0
33	35.485617566986676	37.0	35.0	40.0	30.0	41.0
34	35.14083619907341	36.0	35.0	40.0	29.0	41.0
35	34.75714643931796	35.0	35.0	40.0	25.0	41.0
36	34.31088503606056	35.0	35.0	40.0	22.0	41.0
37	33.761185341739505	35.0	33.0	40.0	20.0	41.0
38	33.18186702966041	35.0	33.0	40.0	15.0	41.0
39	32.634985313082105	35.0	33.0	40.0	12.0	41.0
40	31.89946625591059	35.0	32.0	40.0	10.0	41.0
41	31.278490232602568	35.0	31.0	40.0	7.0	41.0
42	30.559320819601663	35.0	27.0	39.0	7.0	41.0
43	29.620820795720494	35.0	23.0	38.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	6.0
11	7.0
12	4.0
13	4.0
14	5.0
15	4.0
16	2.0
17	10.0
18	18.0
19	47.0
20	114.0
21	244.0
22	430.0
23	726.0
24	1224.0
25	1996.0
26	3008.0
27	4304.0
28	6155.0
29	7923.0
30	9782.0
31	11347.0
32	13571.0
33	16650.0
34	20546.0
35	24473.0
36	33190.0
37	60178.0
38	59091.0
39	59930.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.260925634044995	20.00704494435688	14.056156564932895	14.675872856665235
2	16.489050484787697	26.585112480298033	32.93869704351149	23.98713999140278
3	25.013433156612695	30.46251850790467	30.158332139274968	14.36571619620767
4	13.518531785833693	18.256853895018388	28.05320724076993	40.17140707837799
5	10.084121411854612	46.54738023594594	29.130247886516692	14.23825046568276
6	29.343685819362854	28.89949610736973	20.244961073697283	21.51185699957014
7	22.4700888379424	38.17613554950566	22.29635334575154	17.0574222668004
8	34.93605817452357	28.945467354444286	20.49302669914505	15.62544777188709
9	28.59530973874003	10.693688207479582	24.799995223766537	35.911006830013854
10	20.726166595023166	33.75871662606868	25.6788221808282	19.836294598079952
11	25.336425944500167	26.46600515833214	19.805846109757844	28.39172278740985
12	18.717461909538137	35.81309404403687	29.18248794001051	16.286956106414483
13	30.098927735587715	20.49183264077948	32.53092611166834	16.878313511964464
14	22.321727086019965	25.290753212017	34.18827912308354	18.199240578879497
15	27.6666308449157	24.96865596790371	31.35268901943927	16.01202416774132
16	18.680744614796772	25.71016621292449	35.910708315422454	19.698380856856282
17	15.586043845823184	33.31631800162392	33.81274776711086	17.28489038544204
18	14.677962458804986	24.488047475760617	39.08153030520132	21.75245976023308
19	15.734405597745619	29.66578306347614	41.494125232841384	13.10568610593686
20	16.55711181162535	24.98059655155944	43.613877346324685	14.848414290490519
21	20.30496250656732	25.883006161341164	39.624528346945596	14.187502985145914
22	18.567010555475953	27.662451640636192	39.12899412523284	14.641543678655012
23	16.624576109280223	28.772925920619002	38.93018340736496	15.672314562735826
24	15.600074031618666	30.25475235229498	39.986328031714194	14.158845584372164
25	14.268698954004872	30.629089649902085	39.81348808329751	15.288723312795529
26	16.06008501695563	30.39236757892726	39.26840043941348	14.279146964703635
27	14.214070783779912	32.06703443664327	39.38989587811052	14.328998901466303
28	13.683610354874146	31.83061088025983	40.58813344796294	13.89764531690309
29	14.424225056120743	32.110617566986676	40.426637054019196	13.038520322873381
30	12.740901275254334	33.26407794813011	41.3565100062091	12.638510770406459
31	12.835828915317379	34.36261164445718	38.89824234608588	13.903317094139561
32	12.671645890051106	34.563810479056215	39.66154415627836	13.102999474614318
33	11.847148588623012	34.97576061517887	39.88393752686631	13.293153269331807
34	13.120313320915125	35.06173281750012	37.60537565076181	14.212578210822945
35	12.705975068061326	34.66530544012991	38.34240817691168	14.286311314897072
36	12.335518460142332	35.46472751588098	36.493408797822035	15.706345226154655
37	12.297607107035393	35.28949945073315	36.85073076371973	15.562162678511726
38	12.416415914409896	33.826180923723555	37.42865501265702	16.32874814920953
39	13.20389740650523	34.03723073983856	37.026257343458944	15.73261451019726
40	12.500597029182787	33.954243683431244	37.83851554663992	15.706643740746049
41	12.25491952046616	34.99605960739361	35.75548072789798	16.99354014424225
42	12.385071882313607	34.389776472273965	36.440870229736824	16.784281415675597
43	11.703264555571476	34.88560920857812	35.719061947748	17.6920642881024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	126.0
2	179.0
3	758.0
4	1337.0
5	1337.0
6	2211.0
7	3085.0
8	3436.5
9	3788.0
10	5439.0
11	7090.0
12	7090.0
13	12599.5
14	18109.0
15	24263.5
16	30418.0
17	27156.0
18	23894.0
19	23894.0
20	25641.0
21	27388.0
22	19846.0
23	12304.0
24	10788.5
25	9273.0
26	9273.0
27	9808.0
28	10343.0
29	10305.0
30	10267.0
31	10115.5
32	9964.0
33	9964.0
34	9514.0
35	9064.0
36	8656.5
37	8249.0
38	8952.0
39	9655.0
40	9655.0
41	10651.5
42	11648.0
43	12415.0
44	13182.0
45	15219.5
46	17257.0
47	17257.0
48	18744.0
49	20231.0
50	19221.0
51	18211.0
52	14052.0
53	9893.0
54	9893.0
55	10395.5
56	10898.0
57	9914.0
58	8930.0
59	7780.0
60	6630.0
61	6630.0
62	6167.0
63	5704.0
64	5128.0
65	4552.0
66	4257.5
67	3963.0
68	3963.0
69	3547.0
70	3131.0
71	2777.5
72	2424.0
73	2059.5
74	1695.0
75	1695.0
76	1375.5
77	1056.0
78	822.5
79	589.0
80	437.0
81	285.0
82	285.0
83	215.5
84	146.0
85	98.5
86	51.0
87	37.0
88	23.0
89	23.0
90	16.0
91	9.0
92	6.5
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	334992.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.61316852069454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.97419804128104	34.50567146961203
2	10.081728687748138	8.592288071018642
3	3.2310621662776104	4.1305738977728454
4	1.5620705049083017	2.662590946674555
5	0.8579838393426733	1.8280704966970924
6	0.5470432190748472	1.3986746929523812
7	0.39468639002449735	1.1773186355656935
8	0.31589583737106924	1.0769058042303432
9	0.21898849425389272	0.8398614248760851
>10	1.55245484590899	12.688756017868355
>50	0.14176960295054553	4.179529526904388
>100	0.10316896848381284	8.34231102851313
>500	0.010527445763654372	3.2195087912075664
>1k	0.005614637740615665	3.6726026898308324
>5k	0.0014036594351539162	4.387982627459118
>10k+	0.0014036594351539162	7.297353878816963
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12847	3.8350169556287907	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11553	3.448739074365955	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8001	2.3884152457372116	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6671	1.9913908391842192	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	2348	0.7009122605912977	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1832	0.5468787314323924	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1688	0.5038926302717677	No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	1520	0.4537421789177055	TruSeq Adapter, Index 3 (95% over 21bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1516	0.4525481205521326	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1178	0.3516501886612218	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1152	0.3438888092849978	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1046	0.31224626259731575	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	987	0.29463390170511533	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	916	0.2734393657161962	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	915	0.27314085112480296	No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	798	0.2382146439317954	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	770	0.22985623537278502	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	764	0.22806514782442566	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	759	0.22657257486745952	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	728	0.21731862253426948	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	676	0.20179586378182165	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	650	0.19403448440559776	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	638	0.19045230930887902	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	584	0.17433252137364474	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	558	0.16657114199742082	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	521	0.15552610211587142	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	501	0.14955581028800688	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	494	0.14746620814825429	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	491	0.1465706643740746	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	453	0.13522710990113196	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	453	0.13522710990113196	No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	447	0.1334360223527726	No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA	435	0.12985384725605387	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	371	0.11074891340688733	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG	358	0.10686822371877537	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	356	0.10627119453598892	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	347	0.10358456321344987	No Hit
GAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA	335	0.10000238811673115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.955437741796819E-4	0.0
3	0.0	0.0	0.0	8.955437741796819E-4	0.0
4	0.0	0.0	0.0	8.955437741796819E-4	0.0
5	0.0	0.0	0.0	8.955437741796819E-4	0.0
6	0.0	0.0	0.0	8.955437741796819E-4	0.0
7	0.0	0.0	0.0	0.0014925729569661365	0.0
8	0.0	0.0	0.0	0.0014925729569661365	0.0
9	0.0	0.0	0.0	0.0023881167311458184	0.0
10	0.0	0.0	0.0	0.003880689688111955	0.0
11	0.0	0.0	0.0	0.005373262645078092	0.0
12	0.0	2.985145913932273E-4	0.0	0.006865835602044228	0.0
13	0.0	2.985145913932273E-4	0.0	0.008656923150403591	0.0
14	0.0	2.985145913932273E-4	0.0	0.013433156612695228	0.0
15	0.0	2.985145913932273E-4	0.0	0.021194535988919138	0.0
16	0.0	2.985145913932273E-4	0.0	0.04776233462291637	0.0
17	0.0	2.985145913932273E-4	0.0	0.07850933753641878	0.0
18	0.0	2.985145913932273E-4	0.0	0.09044992119214787	0.0
19	0.0	2.985145913932273E-4	0.0	0.11224148636385346	0.0
20	0.0	2.985145913932273E-4	0.0	0.13045087643884032	0.0
21	0.0	2.985145913932273E-4	0.0	0.16239193771791566	0.0
22	0.0	2.985145913932273E-4	0.0	0.22358742895352726	0.0
23	0.0	2.985145913932273E-4	0.0	0.32179872952189903	0.0
24	0.0	2.985145913932273E-4	0.0	0.49165353202464535	0.0
25	0.0	2.985145913932273E-4	0.0	0.5331470602283039	0.0
26	0.0	2.985145913932273E-4	0.0	0.5973276973778479	0.0
27	0.0	2.985145913932273E-4	0.0	0.6585231886134594	0.0
28	0.0	2.985145913932273E-4	0.0	0.7412117304293834	0.0
29	0.0	2.985145913932273E-4	0.0	0.8662893442231456	0.0
30	0.0	2.985145913932273E-4	0.0	1.078234704112337	0.0
31	0.0	2.985145913932273E-4	0.0	1.6898911018770597	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTCAC	20	0.0018400745	37.0	16
ACTATAT	25	0.005490854	29.599998	3
ATCCCTA	25	0.005490854	29.599998	22
ACCAGCG	25	0.005490854	29.599998	12
CGTCTTC	70	6.557457E-9	26.42857	37
TACACTC	35	8.856225E-4	26.42857	3
CCGTCTT	235	0.0	25.978722	37
GCCGTCT	260	0.0	23.48077	36
ATGCCCC	65	2.6734324E-6	22.76923	37
TATTGAC	60	3.7161473E-5	21.583334	30
TGTTTAT	45	0.003820236	20.555555	35
TTGTTTA	45	0.003820236	20.555555	34
AGAGGCG	45	0.003820236	20.555555	5
ATTGACT	65	6.883486E-5	19.923077	31
TATACAC	605	0.0	19.264462	3
TGGGCAG	440	0.0	18.920454	6
TGCCGTC	315	0.0	18.793652	35
GCAGTCG	415	0.0	18.72289	9
CAGTCGG	420	0.0	18.500002	10
AGTCGGT	420	0.0	18.500002	11
>>END_MODULE