FastQCFastQC Report
Fri 10 Feb 2017
ERR1630638.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630638.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2171616
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59070.27200941602935325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52130.24005164817352606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46270.21306713525779877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32630.15025676731061108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATG609.2404237E-418.52
GGTATCA32100.017.1744561
TAGGACC5150.014.3689324
GTTTTCG3100.014.32258128
TTTCGGA3650.014.19178130
GTACGAC800.00630113.8750013
TCTTATA17750.013.75774837
CAACCGT950.00124569813.6315796
TACGGGT950.00124569813.6315794
CGAACGA2601.8189894E-1213.5192316
TAACGAA2750.013.45454513
ATAGGAC5650.013.4247793
GTATAGA1951.0271833E-813.2820521
GTTCTAG2408.185452E-1113.1041661
TAGTTCG850.009408249513.0588247
TCCGATA2850.012.9824568
GTTAGAC1000.00183339312.9500013
TCTATAC2152.903107E-912.9069773
TATACAG3300.012.8939395
CCTAGTC1153.57945E-412.8695643
Produced by FastQC (version 0.11.5)