FastQCFastQC Report
Fri 10 Feb 2017
ERR1630637.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630637.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664795
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78010.468586222327674No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69010.41452551215014466No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60430.36298763511423326No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42910.25774945263530946No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAT250.005497042629.614
CGAACGA1059.34805E-615.85714416
ACGAACG1059.34805E-615.85714415
GTACTAG1101.4525664E-515.1363651
GGTATCA44000.014.9681821
GTGTAAG2400.014.6458331
CGTGGGT2550.014.50980433
CGATAAC1152.2109114E-514.4782610
GTCTAGC908.276764E-414.3888881
CCTAGAC1304.446556E-614.2307693
TTAGACC1051.6563344E-414.0952394
ATACCGT1601.7846105E-713.8756
GTCGAAA950.00124548413.63157922
CGGTCGA950.00124548413.63157920
ATCATAC950.00124548413.6315793
CTCTAAC1501.3063891E-613.5666671
GGTCGAA1102.4573965E-413.45454721
ACCTTAC1254.8469323E-513.321
TACCGTC1254.8469323E-513.327
TCTTATA19400.013.25515537
Produced by FastQC (version 0.11.5)