##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630637.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1664795
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10617943951057	33.0	31.0	34.0	30.0	34.0
2	32.27262095333059	34.0	31.0	34.0	30.0	34.0
3	32.37323514306566	34.0	31.0	34.0	30.0	34.0
4	35.94031337191666	37.0	35.0	37.0	35.0	37.0
5	35.897857093516016	37.0	35.0	37.0	35.0	37.0
6	35.974558429115895	37.0	35.0	37.0	35.0	37.0
7	35.92301694803264	37.0	35.0	37.0	35.0	37.0
8	35.932093741271444	37.0	35.0	37.0	35.0	37.0
9	37.64438204103208	39.0	37.0	39.0	35.0	39.0
10	37.55755213104316	39.0	37.0	39.0	35.0	39.0
11	37.65425653008328	39.0	37.0	39.0	35.0	39.0
12	37.60436510200956	39.0	37.0	39.0	35.0	39.0
13	37.6411924591316	39.0	37.0	39.0	35.0	39.0
14	38.94975537528644	40.0	38.0	41.0	36.0	41.0
15	38.960187290327035	40.0	38.0	41.0	36.0	41.0
16	38.92451683240279	40.0	38.0	41.0	36.0	41.0
17	38.90992104132941	40.0	38.0	41.0	35.0	41.0
18	38.901327791109416	40.0	38.0	41.0	35.0	41.0
19	38.97241642364375	40.0	38.0	41.0	35.0	41.0
20	38.93805964097682	40.0	38.0	41.0	35.0	41.0
21	38.90193206971429	40.0	38.0	41.0	35.0	41.0
22	38.82956039632507	40.0	38.0	41.0	35.0	41.0
23	38.778491045444035	40.0	38.0	41.0	35.0	41.0
24	38.73016737796546	40.0	38.0	41.0	35.0	41.0
25	38.675943884982836	40.0	38.0	41.0	34.0	41.0
26	38.5420595328554	40.0	38.0	41.0	34.0	41.0
27	38.41055325130121	40.0	38.0	41.0	34.0	41.0
28	38.32502500307846	40.0	38.0	41.0	34.0	41.0
29	38.24741124282569	40.0	38.0	41.0	34.0	41.0
30	38.15939920530756	40.0	38.0	41.0	34.0	41.0
31	38.104761246880244	40.0	37.0	41.0	33.0	41.0
32	38.001689697530324	40.0	37.0	41.0	33.0	41.0
33	37.91944834048637	40.0	37.0	41.0	33.0	41.0
34	37.841797939085595	40.0	37.0	41.0	33.0	41.0
35	37.77736357929955	40.0	37.0	41.0	33.0	41.0
36	37.727750263545964	40.0	37.0	41.0	33.0	41.0
37	37.585174751245646	40.0	37.0	41.0	33.0	41.0
38	37.43298123793019	40.0	36.0	41.0	32.0	41.0
39	37.358566069696266	40.0	36.0	41.0	32.0	41.0
40	37.17316846818978	40.0	36.0	41.0	31.0	41.0
41	37.08976120182965	40.0	36.0	41.0	31.0	41.0
42	36.93611045203764	40.0	36.0	41.0	31.0	41.0
43	36.16939863466673	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	4.0
11	6.0
12	7.0
13	1.0
14	2.0
15	3.0
16	6.0
17	22.0
18	35.0
19	73.0
20	191.0
21	382.0
22	687.0
23	1297.0
24	2138.0
25	3416.0
26	5318.0
27	7837.0
28	11802.0
29	16592.0
30	22411.0
31	29435.0
32	38115.0
33	49477.0
34	65774.0
35	88740.0
36	124972.0
37	197270.0
38	359870.0
39	638905.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.90594637778225	19.112863745986743	12.113323262023252	24.867866614207756
2	18.557660252463517	21.13767761195823	35.0703239738226	25.234338161755655
3	20.766941275051884	23.712889574992715	29.071267032877923	26.448902117077477
4	14.51199697260023	15.576992963097558	34.167990653503885	35.743019410798325
5	14.371559261050159	37.26086395021609	33.92021239852354	14.447364390210208
6	34.71502497304473	34.27064593538544	15.889223598100665	15.125105493469166
7	28.678546007166045	30.597160611366565	21.803405224066626	18.92088815740076
8	27.326247375803025	33.33653693097348	20.186449382656726	19.150766310566766
9	26.898386888475756	13.756348379229873	20.24387387035641	39.10139086193796
10	17.175928567781618	27.051498833189676	32.98273961658943	22.78983298243928
11	34.800500962580976	22.085301793914567	21.612030310038172	21.50216693346628
12	21.48414669674044	25.77110094636276	29.589649176024675	23.155103180872118
13	29.89683414474455	20.35421778657432	25.539781174258692	24.209166894422438
14	22.724179253301457	21.330374010013244	26.137152021720393	29.808294714964905
15	25.617928934193102	27.50290576317204	23.32275144987821	23.55641385275665
16	24.66573962559955	26.5807501824549	25.038037716355465	23.715472475590087
17	23.016107088260114	26.819278049249306	26.475331797608714	23.689283064881863
18	22.846596728125686	25.510528323307074	27.83784189644971	23.805033052117526
19	23.51058238401725	25.965359098267353	27.8087091804096	22.715349337305796
20	23.94096570448614	25.218840758171428	27.88012938529969	22.960064152042744
21	24.380899750419722	25.372072837796843	27.171874014518306	23.075153397265126
22	24.158890433957332	25.540501983727726	27.11901465345583	23.18159292885911
23	23.754155917094895	25.621713183905527	27.21073765839037	23.413393240609203
24	23.447811892755567	26.097087028733267	27.113488447526574	23.341612630984596
25	23.766890217714494	25.76641568481405	27.10249610312381	23.364197994347652
26	23.433996377932417	26.268819884730554	27.11006460254866	23.187119134788368
27	23.577016990079862	25.99226931844461	26.963800347790567	23.466913343684958
28	23.20580011352749	25.928237410612116	27.48044053472049	23.3855219411399
29	22.869302226400247	26.364867746479298	27.612288600097912	23.153541427022546
30	22.851101787307147	26.719325802876632	27.54519325202202	22.884379157794204
31	23.16116999390315	26.662321787367215	26.94752206728156	23.228986151448076
32	22.604044341795838	26.517018611901165	27.409921341666692	23.469015704636305
33	22.77403524157629	26.321258773602757	27.527893824765208	23.376812160055742
34	23.29277778945756	26.02903060136533	27.373700665847743	23.30449094332936
35	22.939100609985015	26.06741370559138	27.733564793262833	23.259920891160775
36	22.701353620115388	26.44217456203316	27.293450544961996	23.563021272889458
37	23.00277211308299	25.698659594724877	27.618175210761684	23.680393081430445
38	22.513883090710866	25.623815544856875	28.04723704720401	23.815064317228245
39	22.43525479113044	25.70172303496827	28.24870329379894	23.614318880102356
40	22.1436873609063	25.5987073483522	28.598776425926314	23.658828864815188
41	21.75408984289357	25.591559321117614	28.899233839601873	23.755116996386942
42	21.926543508359888	25.66646343844137	28.8122561636718	23.59473688952694
43	21.571845182139544	25.338074657840753	28.787928844091915	24.302151315927787
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	295.0
1	290.0
2	285.0
3	749.5
4	1214.0
5	1214.0
6	1765.5
7	2317.0
8	2405.5
9	2494.0
10	3675.0
11	4856.0
12	4856.0
13	8302.0
14	11748.0
15	15680.0
16	19612.0
17	19247.5
18	18883.0
19	18883.0
20	21408.0
21	23933.0
22	22300.5
23	20668.0
24	23129.5
25	25591.0
26	25591.0
27	29350.0
28	33109.0
29	37805.5
30	42502.0
31	48503.5
32	54505.0
33	54505.0
34	60745.0
35	66985.0
36	72658.0
37	78331.0
38	84138.5
39	89946.0
40	89946.0
41	92847.0
42	95748.0
43	98026.0
44	100304.0
45	101907.5
46	103511.0
47	103511.0
48	105345.0
49	107179.0
50	108013.5
51	108848.0
52	108385.5
53	107923.0
54	107923.0
55	104800.5
56	101678.0
57	98800.0
58	95922.0
59	89848.0
60	83774.0
61	83774.0
62	78104.5
63	72435.0
64	64995.5
65	57556.0
66	50349.0
67	43142.0
68	43142.0
69	37568.5
70	31995.0
71	27474.0
72	22953.0
73	18575.5
74	14198.0
75	14198.0
76	11405.0
77	8612.0
78	6975.0
79	5338.0
80	4254.5
81	3171.0
82	3171.0
83	2351.0
84	1531.0
85	1205.5
86	880.0
87	732.5
88	585.0
89	585.0
90	393.5
91	202.0
92	114.0
93	26.0
94	16.0
95	6.0
96	6.0
97	4.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1664795.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.96413696997219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.46587836899553	62.06940572776327
2	8.516678438274324	12.087574704458163
3	1.9088571345083034	4.063811974480676
4	0.713353067808273	2.024899392475846
5	0.3385560872631309	1.2012670274279338
6	0.20392619449913185	0.8682867838920949
7	0.13201963999374167	0.6558061870669613
8	0.09144995344664912	0.5191733617828471
9	0.07007738991621973	0.44756833468614887
>10	0.4590625993534894	6.42581554411951
>50	0.06208921597726874	3.0858887563199526
>100	0.036445488854274934	4.391494635472474
>500	0.0010145817534793935	0.4529931956955451
>1k	3.3819391782646444E-4	0.46133307042424454
>5k	2.536454383698484E-4	1.2446813039343156
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7801	0.468586222327674	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6901	0.41452551215014466	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6043	0.36298763511423326	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4291	0.25774945263530946	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.006745575281041E-5	0.0
2	0.0	1.2013491150562082E-4	0.0	0.0016818887610786915	0.0
3	0.0	1.2013491150562082E-4	0.0	0.0025829005973708476	0.0
4	0.0	1.2013491150562082E-4	0.0	0.004625194092966401	0.0
5	0.0	1.802023672584312E-4	0.0	0.005406071017752936	0.0
6	0.0	1.802023672584312E-4	0.0	0.0055862733850113675	0.0
7	0.0	1.802023672584312E-4	0.0	0.006487285221303524	0.0
8	0.0	1.802023672584312E-4	0.0	0.006967824867326007	0.0
9	0.0	1.802023672584312E-4	0.0	0.011292681681528357	0.0
10	0.0	1.802023672584312E-4	0.0	0.017299427256809396	0.0
11	0.0	1.802023672584312E-4	0.0	0.02090347460197802	0.0
12	0.0	1.802023672584312E-4	0.0	0.022585363363056714	0.0
13	0.0	1.802023672584312E-4	0.0	0.023786712478112922	0.0
14	0.0	1.802023672584312E-4	0.0	0.0254085337834388	0.0
15	0.0	1.802023672584312E-4	0.0	0.027390759823281544	0.0
16	0.0	1.802023672584312E-4	0.0	0.03147534681447265	0.0
17	0.0	1.802023672584312E-4	0.0	0.03670121546496716	0.0
18	6.006745575281041E-5	1.802023672584312E-4	0.0	0.038923711327821145	0.0
19	6.006745575281041E-5	1.802023672584312E-4	0.0	0.04324856814202349	0.0
20	6.006745575281041E-5	1.802023672584312E-4	0.0	0.046492210752675255	0.0
21	6.006745575281041E-5	1.802023672584312E-4	0.0	0.05249895632795629	0.0
22	6.006745575281041E-5	1.802023672584312E-4	0.0	0.06192954688114753	0.0
23	6.006745575281041E-5	2.4026982301124164E-4	0.0	0.0756249267927883	0.0
24	6.006745575281041E-5	2.4026982301124164E-4	0.0	0.09821029015584501	0.0
25	6.006745575281041E-5	2.4026982301124164E-4	0.0	0.10385663099660919	0.0
26	6.006745575281041E-5	2.4026982301124164E-4	0.0	0.11376776119582291	0.0
27	6.006745575281041E-5	2.4026982301124164E-4	0.0	0.13455110088629532	0.0
28	6.006745575281041E-5	2.4026982301124164E-4	0.0	0.17335467730261084	0.0
29	6.006745575281041E-5	2.4026982301124164E-4	0.0	0.2284966016836908	0.0
30	6.006745575281041E-5	2.4026982301124164E-4	0.0	0.3088067900251983	0.0
31	6.006745575281041E-5	2.4026982301124164E-4	0.0	0.5638532071516312	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTAAT	25	0.0054970426	29.6	14
CGAACGA	105	9.34805E-6	15.857144	16
ACGAACG	105	9.34805E-6	15.857144	15
GTACTAG	110	1.4525664E-5	15.136365	1
GGTATCA	4400	0.0	14.968182	1
GTGTAAG	240	0.0	14.645833	1
CGTGGGT	255	0.0	14.509804	33
CGATAAC	115	2.2109114E-5	14.47826	10
GTCTAGC	90	8.276764E-4	14.388888	1
CCTAGAC	130	4.446556E-6	14.230769	3
TTAGACC	105	1.6563344E-4	14.095239	4
ATACCGT	160	1.7846105E-7	13.875	6
GTCGAAA	95	0.001245484	13.631579	22
CGGTCGA	95	0.001245484	13.631579	20
ATCATAC	95	0.001245484	13.631579	3
CTCTAAC	150	1.3063891E-6	13.566667	1
GGTCGAA	110	2.4573965E-4	13.454547	21
ACCTTAC	125	4.8469323E-5	13.32	1
TACCGTC	125	4.8469323E-5	13.32	7
TCTTATA	1940	0.0	13.255155	37
>>END_MODULE