FastQCFastQC Report
Fri 10 Feb 2017
ERR1630636.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630636.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320582
Sequences flagged as poor quality0
Sequence length43
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT116783.642749748894199No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT114783.5803632144038033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT92012.8700925192306492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59851.8669170446250882No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17280.5390196579970179No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16140.5034593333374925No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13730.42828355927656575No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA11580.3612180346993905No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10460.326281575384769No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10350.3228503159877972No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT10040.3131804031417859No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA9400.2932167121048593No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8170.254848993393266No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT6890.21492161131941284No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA6780.21149035192244103No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6680.2083710251979213No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.1940221222651303No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5840.18216868071195513No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5740.17904935398743535No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT5640.17593002726291557TruSeq Adapter, Index 5 (95% over 21bp)
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5100.15908566295050877No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA4920.15347087484637317No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA4890.15253507682901724No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA4760.14847995208714151No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4500.1403697026033901No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA3910.12196567492872337No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA3560.11104803139290415No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA3500.1091764353581923No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC3460.10792870466838438No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA3440.10730483932348042No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG3250.10137811854689285No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA3230.10075425320198889No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA3220.1004423205295369No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA3220.1004423205295369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA308.293284E-637.02
ATAACCG200.001839956737.04
GCCAGAG200.001839956737.02
GCGTTAT401.5953065E-632.3751
TAATAGC250.00549050629.5999984
ACGGGCC250.00549050629.59999817
TATACCA405.926977E-527.7500025
GTTATAC405.926977E-527.7500023
TAACCGG358.8553916E-426.428575
CGTCAGA358.8553916E-426.428571
TATAAAC451.3203057E-424.6666682
CCGTCTT652.6728358E-622.7692337
ATGCCCC753.7233804E-722.237
CTTTGCG705.0830076E-621.1428579
GCCGTCT705.0830076E-621.14285736
CAGATGT450.00381988120.5555574
ATACCCC450.00381988120.55555719
CAGACCA450.00381988120.5555574
AAGTACT555.131982E-420.1818184
GGAAAGT1202.3646862E-1020.0416688
Produced by FastQC (version 0.11.5)