##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630636.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	320582
Sequences flagged as poor quality	0
Sequence length	43
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.923623285150132	33.0	31.0	34.0	30.0	34.0
2	31.947626504295314	33.0	31.0	34.0	30.0	34.0
3	31.93608499541459	33.0	31.0	34.0	30.0	34.0
4	35.62993243538315	37.0	35.0	37.0	33.0	37.0
5	35.65772563649862	37.0	35.0	37.0	33.0	37.0
6	35.82437254742936	37.0	35.0	37.0	35.0	37.0
7	35.70810588242634	37.0	35.0	37.0	33.0	37.0
8	35.82344298806546	37.0	35.0	37.0	35.0	37.0
9	37.346494812559655	39.0	37.0	39.0	34.0	39.0
10	37.305207404033915	39.0	37.0	39.0	34.0	39.0
11	37.460659051350355	39.0	37.0	39.0	35.0	39.0
12	37.47082805647229	39.0	37.0	39.0	35.0	39.0
13	37.51815760086343	39.0	37.0	39.0	35.0	39.0
14	38.563553162685366	40.0	38.0	41.0	34.0	41.0
15	38.47794947938437	40.0	38.0	41.0	34.0	41.0
16	38.33920182667773	40.0	38.0	41.0	34.0	41.0
17	38.55077328109501	40.0	38.0	41.0	34.0	41.0
18	38.67998827133152	40.0	38.0	41.0	35.0	41.0
19	38.85142958743785	40.0	38.0	41.0	35.0	41.0
20	38.871212357524755	40.0	38.0	41.0	35.0	41.0
21	38.83884934275786	40.0	38.0	41.0	35.0	41.0
22	38.700787941930614	40.0	38.0	41.0	35.0	41.0
23	38.57116431989319	40.0	38.0	41.0	34.0	41.0
24	38.433798528925514	40.0	38.0	41.0	34.0	41.0
25	38.34129177558316	40.0	37.0	41.0	34.0	41.0
26	38.00917082057009	40.0	37.0	41.0	34.0	41.0
27	37.696882544871514	40.0	35.0	41.0	33.0	41.0
28	37.49438209256914	39.0	35.0	41.0	33.0	41.0
29	37.25240967989469	39.0	35.0	41.0	33.0	41.0
30	36.91563468940864	39.0	35.0	41.0	33.0	41.0
31	36.52047526061975	38.0	35.0	40.0	32.0	41.0
32	36.11972911766724	38.0	35.0	40.0	31.0	41.0
33	35.76370476196418	37.0	35.0	40.0	30.0	41.0
34	35.44541490164763	37.0	35.0	40.0	30.0	41.0
35	35.08762500701849	37.0	35.0	40.0	27.0	41.0
36	34.71021766661884	37.0	35.0	40.0	24.0	41.0
37	34.19004186136464	36.0	34.0	40.0	21.0	41.0
38	33.665255691211605	35.0	33.0	40.0	18.0	41.0
39	33.16406410840284	35.0	33.0	40.0	15.0	41.0
40	32.53660217978552	35.0	33.0	40.0	10.0	41.0
41	31.981112476683034	35.0	32.0	40.0	8.0	41.0
42	31.34945817294795	35.0	31.0	40.0	7.0	41.0
43	30.494120069124282	35.0	27.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	5.0
12	4.0
13	1.0
14	1.0
15	6.0
16	5.0
17	3.0
18	16.0
19	45.0
20	115.0
21	226.0
22	405.0
23	672.0
24	1124.0
25	1758.0
26	2699.0
27	4034.0
28	5538.0
29	7312.0
30	9061.0
31	10456.0
32	12325.0
33	15235.0
34	18458.0
35	22433.0
36	31396.0
37	56901.0
38	60181.0
39	60161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.97827700869044	19.398780967116057	14.155192743198308	14.46774928099519
2	16.31782196130787	26.369852331072863	34.144462259265964	23.167863448353305
3	24.676681785003524	31.238185550030884	29.4589215863648	14.626211078600795
4	13.105851233069854	17.62045280146733	29.878471030812708	39.3952249346501
5	10.024268361916763	46.574979256477285	28.76674298619386	14.634009395412095
6	29.250238628494422	29.442701087397293	20.25347648963448	21.053583794473802
7	22.933602011341872	38.15154936958407	22.44761090766169	16.46723771141237
8	34.85254942573195	28.122913950252975	20.41942467137893	16.605111952636143
9	30.128641034119198	10.571398269397534	24.42838337773175	34.87157731875152
10	20.34487276266291	33.32688672476933	25.93720171438197	20.391038798185797
11	25.63899407951788	27.152803338927328	19.48581018273016	27.722392398824635
12	20.502710694923607	34.699078550885574	29.626117498799058	15.172093255391756
13	30.238129402149838	21.028629180677644	31.875775932522725	16.857465484649794
14	23.798279379378755	24.009769731301194	34.343787236962775	17.848163652357275
15	28.037132465328686	23.568073067109196	32.32901410559545	16.065780361966674
16	18.616141891934046	25.734445477288183	35.9246620209494	19.724750609828376
17	16.048624064981816	30.664541365391695	34.598636230356036	18.68819833927045
18	14.955923913382534	23.403060683382098	40.14199175250014	21.499023650735225
19	16.19336082499953	27.828761440130762	42.581929116419516	13.395948618450193
20	18.08024156066155	23.01844769824881	43.900156590201576	15.001154150888071
21	21.159952835779926	23.743691161699658	40.277994397689206	14.818361604831212
22	19.50203068169766	24.92373246158549	39.75550717133214	15.818729685384705
23	17.194976636242835	26.809053533885248	39.66379896563126	16.332170864240663
24	15.441915017062719	28.3085138903619	40.95582409492735	15.293746997648029
25	15.129358479265836	27.899258224104912	40.19626803750678	16.775115259122472
26	16.78603290265829	27.458809290602716	40.104871764478354	15.650286042260639
27	14.83832529586814	29.31106549962256	40.030007923089876	15.82060128141942
28	14.637440654809067	29.176934450468213	41.36819908790886	14.817425806813858
29	14.232863978638852	30.244991920943782	41.467393677748596	14.054750422668771
30	13.51978588941363	31.408500789189663	41.15702066865888	13.914692652737834
31	13.59090653873268	32.06979805478786	39.87497738488125	14.464318021598219
32	12.421159017037763	33.0860747016364	40.46952105857471	14.023245222751122
33	12.668833558964632	32.77507782720178	40.481686432800345	14.074402181033246
34	13.263689165330556	33.26450019027893	38.37395736504233	15.097853279348186
35	12.776762263633017	33.7168025653343	38.03145529069006	15.474979880342627
36	12.93335246520391	34.09798429107062	37.00613259634041	15.962530647385067
37	12.908397851407752	34.54311221465959	36.91723178469159	15.631258149241066
38	13.112401819191346	33.47287121547685	37.301220904479976	16.113506060851826
39	13.702890368142938	33.6622143476552	37.23041218783338	15.404483096368478
40	12.972344049260407	33.70494912378113	37.247256552145785	16.075450274812685
41	12.470444379285176	35.031286847046935	36.083435751227455	16.414833022440437
42	12.600832236370103	34.47074383465073	36.23409923202176	16.69432469695741
43	12.0643080397527	35.43586352321715	35.25088744845313	17.248940988577026
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17.0
1	80.5
2	144.0
3	700.0
4	1256.0
5	1256.0
6	2092.5
7	2929.0
8	3142.0
9	3355.0
10	4710.5
11	6066.0
12	6066.0
13	10478.0
14	14890.0
15	21169.0
16	27448.0
17	24714.5
18	21981.0
19	21981.0
20	22612.5
21	23244.0
22	16501.0
23	9758.0
24	9022.5
25	8287.0
26	8287.0
27	8777.0
28	9267.0
29	9653.0
30	10039.0
31	10335.0
32	10631.0
33	10631.0
34	10405.5
35	10180.0
36	9947.5
37	9715.0
38	10473.5
39	11232.0
40	11232.0
41	12089.5
42	12947.0
43	13603.5
44	14260.0
45	16146.0
46	18032.0
47	18032.0
48	19532.5
49	21033.0
50	18760.5
51	16488.0
52	14088.5
53	11689.0
54	11689.0
55	10341.5
56	8994.0
57	8969.0
58	8944.0
59	7917.5
60	6891.0
61	6891.0
62	6059.0
63	5227.0
64	4775.0
65	4323.0
66	3891.5
67	3460.0
68	3460.0
69	3070.5
70	2681.0
71	2318.0
72	1955.0
73	1655.0
74	1355.0
75	1355.0
76	1137.0
77	919.0
78	701.5
79	484.0
80	374.0
81	264.0
82	264.0
83	197.0
84	130.0
85	89.0
86	48.0
87	31.0
88	14.0
89	14.0
90	8.5
91	3.0
92	2.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	320582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.75221368205868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.48182610901246	37.73726133025945
2	9.71895494636126	8.89327406944443
3	2.9128155560203326	3.9980327920640053
4	1.3136254481736123	2.4040508881211684
5	0.7380993409133634	1.688483938202744
6	0.5018254298876714	1.377577457958702
7	0.32356173457149756	1.0362565933616898
8	0.23605113665093835	0.8639889635157253
9	0.20667180130478294	0.8510123173817163
>10	1.3271858756978758	11.859096319308057
>50	0.1268523292921813	4.005228726146104
>100	0.09820825493588228	8.45291727884839
>500	0.0068200177038807135	2.0737574099382208
>1k	0.0047740123927165	2.795172867623621
>5k	0.0013640035407761427	4.738501358309032
>10k+	0.0013640035407761427	7.22538768951692
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11678	3.642749748894199	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11478	3.5803632144038033	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9201	2.8700925192306492	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5985	1.8669170446250882	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1728	0.5390196579970179	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1614	0.5034593333374925	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1373	0.42828355927656575	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1158	0.3612180346993905	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	1046	0.326281575384769	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1035	0.3228503159877972	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1004	0.3131804031417859	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	940	0.2932167121048593	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	817	0.254848993393266	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	689	0.21492161131941284	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	678	0.21149035192244103	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	668	0.2083710251979213	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	622	0.1940221222651303	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	584	0.18216868071195513	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	574	0.17904935398743535	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	564	0.17593002726291557	TruSeq Adapter, Index 5 (95% over 21bp)
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	510	0.15908566295050877	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	492	0.15347087484637317	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	489	0.15253507682901724	No Hit
GAACAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAA	476	0.14847995208714151	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	450	0.1403697026033901	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	391	0.12196567492872337	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	356	0.11104803139290415	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	350	0.1091764353581923	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	346	0.10792870466838438	No Hit
GTACATGGGAATGGTATCAACGCAGAAAAAAAAAAAAAAAAAA	344	0.10730483932348042	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	325	0.10137811854689285	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	323	0.10075425320198889	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	322	0.1004423205295369	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	322	0.1004423205295369	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.1193267245197795E-4	0.0
2	0.0	0.0	0.0	0.0034312593969717574	0.0
3	0.0	0.0	0.0	0.005302855431683625	0.0
4	0.0	0.0	0.0	0.009357980173559339	0.0
5	0.0	0.0	0.0	0.009981845518463294	0.0
6	0.0	0.0	0.0	0.009981845518463294	0.0
7	0.0	0.0	0.0	0.01060571086336725	0.0
8	0.0	0.0	0.0	0.01060571086336725	0.0
9	0.0	0.0	0.0	0.01715629698485879	0.0
10	0.0	0.0	0.0	0.022147219744090434	0.0
11	0.0	0.0	0.0	0.02776200784822604	0.0
12	0.0	0.0	0.0	0.034000661297265596	0.0
13	0.0	0.0	0.0	0.03618419000442944	0.0
14	0.0	0.0	0.0	0.040863180091209116	0.0
15	0.0	0.0	0.0	0.05365241966174021	0.0
16	0.0	0.0	0.0	0.08141442750996625	0.0
17	0.0	0.0	0.0	0.12352533829098328	0.0
18	0.0	0.0	0.0	0.14504869269016976	0.0
19	0.0	0.0	0.0	0.17530616191801163	0.0
20	0.0	0.0	0.0	0.19495792028248624	0.0
21	0.0	0.0	0.0	0.2489222726166784	0.0
22	0.0	0.0	0.0	0.34499753573188763	0.0
23	0.0	0.0	0.0	0.5084502560967241	0.0
24	0.0	0.0	0.0	0.7330417802621482	0.0
25	0.0	0.0	0.0	0.7863822672514365	0.0
26	0.0	0.0	0.0	0.8930632412300129	0.0
27	0.0	0.0	0.0	0.948899189598917	0.0
28	0.0	0.0	0.0	1.0125334547791205	0.0
29	0.0	0.0	0.0	1.1098564485841376	0.0
30	0.0	0.0	0.0	1.3085575609360476	0.0
31	0.0	0.0	0.0	1.826989662551235	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATA	30	8.293284E-6	37.0	2
ATAACCG	20	0.0018399567	37.0	4
GCCAGAG	20	0.0018399567	37.0	2
GCGTTAT	40	1.5953065E-6	32.375	1
TAATAGC	25	0.005490506	29.599998	4
ACGGGCC	25	0.005490506	29.599998	17
TATACCA	40	5.926977E-5	27.750002	5
GTTATAC	40	5.926977E-5	27.750002	3
TAACCGG	35	8.8553916E-4	26.42857	5
CGTCAGA	35	8.8553916E-4	26.42857	1
TATAAAC	45	1.3203057E-4	24.666668	2
CCGTCTT	65	2.6728358E-6	22.76923	37
ATGCCCC	75	3.7233804E-7	22.2	37
CTTTGCG	70	5.0830076E-6	21.142857	9
GCCGTCT	70	5.0830076E-6	21.142857	36
CAGATGT	45	0.003819881	20.555557	4
ATACCCC	45	0.003819881	20.555557	19
CAGACCA	45	0.003819881	20.555557	4
AAGTACT	55	5.131982E-4	20.181818	4
GGAAAGT	120	2.3646862E-10	20.041668	8
>>END_MODULE