FastQCFastQC Report
Fri 10 Feb 2017
ERR1630635.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630635.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185480
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61470.5185241421196477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50270.42404764314876675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46310.39064345244120524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32940.2778621317947161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGAC555.1432254E-420.1818183
CGTACAC500.0070350118.4999983
CGAGCCG1950.018.02564215
CGTATGC1156.405389E-817.69565231
TGTACGA752.067336E-417.26666627
TCGCTAT650.001579973517.07692313
CGAACGA1107.808103E-716.81818216
GGTATCA28850.016.2876951
TAACGAA1151.2421988E-616.08695613
TACGACG1059.343514E-615.8571425
GACGGTA1059.343514E-615.8571428
ATACCGC2100.015.85714227
ACGGTAT1059.343514E-615.8571429
TACCGCA2003.6379788E-1215.72499928
CGGACAT3100.015.516129521
TTTCGGA1201.9355266E-615.41666630
ACGAACG1201.9355266E-615.41666615
ACGGACC1455.3451004E-815.3103458
GACGGAC1353.9721454E-715.0740757
ATCTCGT2600.014.94230737
Produced by FastQC (version 0.11.5)