##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630635.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1185480
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05164321624996	33.0	31.0	34.0	30.0	34.0
2	32.22049127779465	33.0	31.0	34.0	30.0	34.0
3	32.31403903903904	34.0	31.0	34.0	30.0	34.0
4	35.892595404393155	37.0	35.0	37.0	35.0	37.0
5	35.854993757802745	37.0	35.0	37.0	35.0	37.0
6	35.93970459223268	37.0	35.0	37.0	35.0	37.0
7	35.88445777237912	37.0	35.0	37.0	35.0	37.0
8	35.89057849984816	37.0	35.0	37.0	35.0	37.0
9	37.59262071059824	39.0	37.0	39.0	35.0	39.0
10	37.50235516415292	39.0	37.0	39.0	35.0	39.0
11	37.600578668556196	39.0	37.0	39.0	35.0	39.0
12	37.55590899888653	39.0	37.0	39.0	35.0	39.0
13	37.59672368998212	39.0	37.0	39.0	35.0	39.0
14	38.88097563855991	40.0	38.0	41.0	35.0	41.0
15	38.88927944798731	40.0	38.0	41.0	35.0	41.0
16	38.84335712116611	40.0	38.0	41.0	35.0	41.0
17	38.8412221209974	40.0	38.0	41.0	35.0	41.0
18	38.83221142490805	40.0	38.0	41.0	35.0	41.0
19	38.90435772851503	40.0	38.0	41.0	35.0	41.0
20	38.876078044336474	40.0	38.0	41.0	35.0	41.0
21	38.83040118770456	40.0	38.0	41.0	35.0	41.0
22	38.76242534669501	40.0	38.0	41.0	35.0	41.0
23	38.70406670715659	40.0	38.0	41.0	34.0	41.0
24	38.6555268751898	40.0	38.0	41.0	34.0	41.0
25	38.60101224820326	40.0	38.0	41.0	34.0	41.0
26	38.467573472348754	40.0	38.0	41.0	34.0	41.0
27	38.332369166919726	40.0	38.0	41.0	34.0	41.0
28	38.233919593751054	40.0	38.0	41.0	34.0	41.0
29	38.157558119917674	40.0	38.0	41.0	33.0	41.0
30	38.06899905523501	40.0	37.0	41.0	33.0	41.0
31	38.00726962917974	40.0	37.0	41.0	33.0	41.0
32	37.89475908492762	40.0	37.0	41.0	33.0	41.0
33	37.80966697034113	40.0	37.0	41.0	33.0	41.0
34	37.74643266862368	40.0	37.0	41.0	33.0	41.0
35	37.68478506596484	40.0	37.0	41.0	33.0	41.0
36	37.635588959746265	40.0	37.0	41.0	33.0	41.0
37	37.480682930121134	40.0	37.0	41.0	32.0	41.0
38	37.32932061274758	40.0	36.0	41.0	32.0	41.0
39	37.25142895704693	40.0	36.0	41.0	32.0	41.0
40	37.05717515268077	40.0	36.0	41.0	31.0	41.0
41	36.984082397003746	40.0	36.0	41.0	31.0	41.0
42	36.82783935621014	39.0	35.0	41.0	31.0	41.0
43	36.038362519823195	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	2.0
11	6.0
12	1.0
13	0.0
14	4.0
15	3.0
16	4.0
17	7.0
18	34.0
19	70.0
20	150.0
21	300.0
22	532.0
23	940.0
24	1669.0
25	2660.0
26	3982.0
27	6041.0
28	8821.0
29	12246.0
30	16716.0
31	21656.0
32	28089.0
33	36693.0
34	48352.0
35	64770.0
36	91309.0
37	144372.0
38	264074.0
39	431972.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.76691298039613	19.709400411647604	12.154570300637717	24.369116307318556
2	18.799895401018997	21.585265040321218	34.69894051354726	24.91589904511253
3	20.982302527246347	23.91394203191956	29.339929817457904	25.763825623376185
4	14.603536120390054	15.684701555488074	34.39771231906064	35.31405000506124
5	14.06113979147687	37.695785673313765	33.9946688261295	14.248405709079867
6	34.41652326483787	34.287799034990044	15.93008739076155	15.365590309410534
7	28.261463710901914	31.113135607517627	21.687417754833486	18.937982926746972
8	27.398859533690995	32.815230961298376	20.493470999088977	19.292438505921652
9	26.869031953301615	13.679775280898877	20.672470223032022	38.77872254276748
10	17.23647805108479	27.24432297466005	32.96124776461855	22.557951209636602
11	34.33664001079732	22.169585315652732	21.656375476600196	21.837399196949757
12	21.460927219354186	26.5360866484462	29.213567500084352	22.78941863211526
13	29.847066167290887	20.44159327867193	25.84126261092553	23.87007794311165
14	22.923794581097955	21.809393663326247	26.036626514154605	29.230185241421196
15	25.72822822822823	27.602068360495323	23.578887876640685	23.09081553463576
16	24.535462428720855	26.68125991159699	25.155127037149512	23.628150622532644
17	22.758798123966663	26.8749367344873	26.526892060599927	23.839373080946114
18	22.49763808752573	25.214259203023246	28.327175490096838	23.960927219354186
19	23.146489185815028	25.954550055673653	28.302712825184734	22.596247933326584
20	24.088133076897122	24.944157640786855	28.21768397611094	22.75002530620508
21	24.34288220805075	25.55563990957249	27.20417046259743	22.89730741977933
22	24.148361844991058	25.457536187873263	27.467017579377128	22.927084387758544
23	23.44569288389513	25.608276816141984	27.591271046327225	23.354759253635656
24	23.57475452981071	26.059655160778757	27.445591659074804	22.919998650335728
25	23.636501670209537	25.720130242602153	27.476380875257277	23.16698721193103
26	23.3685089583966	26.17867867867868	27.33517225090259	23.117640112022134
27	23.488291662448965	25.842274859128793	27.21716098120592	23.452272497216317
28	22.904899281303777	25.91920572257651	27.848803860039816	23.3270911360799
29	22.502783682558963	26.39285352768499	28.032020784829776	23.072342004926274
30	22.631592266423727	26.868441475183047	27.852009312683467	22.647956945709755
31	23.00173769274893	26.83596517866181	27.275533960927216	22.886763167662043
32	22.337702871410738	26.601207949522554	27.53787495360529	23.523214225461416
33	22.41446502682458	26.278722542767486	28.010763572561327	23.29604885784661
34	22.924553767250398	26.027853696393024	27.672335256604917	23.37525727975166
35	22.743023922799203	26.153119411546378	28.160238890575968	22.943617775078447
36	22.577436987549344	26.689273543206127	27.431841954313864	23.30144751493066
37	22.991952626784087	25.943077909370043	27.58308870668421	23.481880757161655
38	22.510459898100347	25.911951277119815	28.248641900327293	23.32894692445254
39	22.541080406248945	25.950669770894493	28.147164017950534	23.36108580490603
40	21.94511927657995	25.645139521544014	28.98134089145325	23.42840031042278
41	21.69745588284914	25.77909370044201	28.996608968519084	23.52684144818976
42	22.066420352937207	25.712116610993014	28.87117454533185	23.350288490737928
43	21.496946384586835	25.374869251273747	28.892009987515603	24.236174376623815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	197.0
1	201.0
2	205.0
3	550.0
4	895.0
5	895.0
6	1311.0
7	1727.0
8	1762.5
9	1798.0
10	2631.5
11	3465.0
12	3465.0
13	6042.5
14	8620.0
15	11337.5
16	14055.0
17	13794.0
18	13533.0
19	13533.0
20	15681.5
21	17830.0
22	16764.5
23	15699.0
24	17386.0
25	19073.0
26	19073.0
27	21660.5
28	24248.0
29	28712.5
30	33177.0
31	36703.5
32	40230.0
33	40230.0
34	43559.0
35	46888.0
36	50451.0
37	54014.0
38	58471.0
39	62928.0
40	62928.0
41	66016.5
42	69105.0
43	69021.5
44	68938.0
45	70548.0
46	72158.0
47	72158.0
48	74206.5
49	76255.0
50	76591.0
51	76927.0
52	79822.0
53	82717.0
54	82717.0
55	80057.0
56	77397.0
57	73478.0
58	69559.0
59	65146.0
60	60733.0
61	60733.0
62	56026.0
63	51319.0
64	44922.0
65	38525.0
66	33570.0
67	28615.0
68	28615.0
69	24214.0
70	19813.0
71	17105.5
72	14398.0
73	11455.0
74	8512.0
75	8512.0
76	6676.0
77	4840.0
78	3956.5
79	3073.0
80	2443.5
81	1814.0
82	1814.0
83	1326.0
84	838.0
85	738.0
86	638.0
87	547.5
88	457.0
89	457.0
90	343.5
91	230.0
92	128.5
93	27.0
94	17.0
95	7.0
96	7.0
97	4.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1185480.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.18057946522484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.49252484598537	58.56486572648119
2	7.507086116182555	9.936466185286115
3	1.59679614405352	3.1703068230389575
4	0.662908307248085	1.7548662362395828
5	0.36115899793664125	1.195085588126343
6	0.23078233387589633	0.9163985151746283
7	0.14916390384284117	0.6910227514130014
8	0.11327789988404327	0.5997437643943768
9	0.08990708569803676	0.5355092726573131
>10	0.6307985965019842	8.49858408286002
>50	0.10112987845685605	4.621759671388944
>100	0.062300043496131184	7.1645802871077455
>500	0.001656235817359849	0.7404620725079984
>1k	2.5480551036305367E-4	0.6682033619478351
>5k	2.5480551036305367E-4	0.942145661376039
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6147	0.5185241421196477	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5027	0.42404764314876675	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4631	0.39064345244120524	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3294	0.2778621317947161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	8.43540169382866E-5	0.0
2	0.0	8.43540169382866E-5	0.0	0.0021932044403954517	0.0
3	0.0	8.43540169382866E-5	0.0	0.0035428687114080373	0.0
4	0.0	8.43540169382866E-5	0.0	0.006917029388939501	0.0
5	0.0	8.43540169382866E-5	0.0	0.007760569558322368	0.0
6	0.0	8.43540169382866E-5	0.0	0.008013631609137227	0.0
7	0.0	8.43540169382866E-5	0.0	0.008857171778520093	0.0
8	8.43540169382866E-5	8.43540169382866E-5	0.0	0.009278941863211525	0.0
9	8.43540169382866E-5	1.687080338765732E-4	0.0	0.01391841279481729	0.0
10	8.43540169382866E-5	1.687080338765732E-4	0.0	0.020244964065188786	0.0
11	8.43540169382866E-5	1.687080338765732E-4	0.0	0.02235381448864595	0.0
12	8.43540169382866E-5	1.687080338765732E-4	0.0	0.024968789013732832	0.0
13	8.43540169382866E-5	1.687080338765732E-4	0.0	0.026487161318621992	0.0
14	8.43540169382866E-5	1.687080338765732E-4	0.0	0.02800553362351115	0.0
15	1.687080338765732E-4	1.687080338765732E-4	0.0	0.03129534028410433	0.0
16	1.687080338765732E-4	1.687080338765732E-4	0.0	0.03770624557141411	0.0
17	1.687080338765732E-4	1.687080338765732E-4	0.0	0.04293619462158788	0.0
18	1.687080338765732E-4	1.687080338765732E-4	0.0	0.04639470931605763	0.0
19	1.687080338765732E-4	1.687080338765732E-4	0.0	0.05111853426460168	0.0
20	1.687080338765732E-4	1.687080338765732E-4	0.0	0.05508317306070115	0.0
21	1.687080338765732E-4	1.687080338765732E-4	0.0	0.062000202449640655	0.0
22	1.687080338765732E-4	1.687080338765732E-4	0.0	0.07372541080406249	0.0
23	1.687080338765732E-4	1.687080338765732E-4	0.0	0.08949961197152208	0.0
24	1.687080338765732E-4	1.687080338765732E-4	0.0	0.1126969666295509	0.0
25	1.687080338765732E-4	1.687080338765732E-4	0.0	0.1183486857644161	0.0
26	1.687080338765732E-4	1.687080338765732E-4	0.0	0.12931470796639336	0.0
27	1.687080338765732E-4	2.530620508148598E-4	0.0	0.14576374126935926	0.0
28	1.687080338765732E-4	2.530620508148598E-4	0.0	0.17343185882511725	0.0
29	1.687080338765732E-4	2.530620508148598E-4	0.0	0.2089448999561359	0.0
30	1.687080338765732E-4	2.530620508148598E-4	0.0	0.27204170462597427	0.0
31	1.687080338765732E-4	2.530620508148598E-4	0.0	0.4680804399905524	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGAC	55	5.1432254E-4	20.181818	3
CGTACAC	50	0.00703501	18.499998	3
CGAGCCG	195	0.0	18.025642	15
CGTATGC	115	6.405389E-8	17.695652	31
TGTACGA	75	2.067336E-4	17.266666	27
TCGCTAT	65	0.0015799735	17.076923	13
CGAACGA	110	7.808103E-7	16.818182	16
GGTATCA	2885	0.0	16.287695	1
TAACGAA	115	1.2421988E-6	16.086956	13
TACGACG	105	9.343514E-6	15.857142	5
GACGGTA	105	9.343514E-6	15.857142	8
ATACCGC	210	0.0	15.857142	27
ACGGTAT	105	9.343514E-6	15.857142	9
TACCGCA	200	3.6379788E-12	15.724999	28
CGGACAT	310	0.0	15.5161295	21
TTTCGGA	120	1.9355266E-6	15.416666	30
ACGAACG	120	1.9355266E-6	15.416666	15
ACGGACC	145	5.3451004E-8	15.310345	8
GACGGAC	135	3.9721454E-7	15.074075	7
ATCTCGT	260	0.0	14.942307	37
>>END_MODULE