FastQCFastQC Report
Fri 10 Feb 2017
ERR1630634.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630634.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1547719
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70210.45363531752210834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61400.39671283999227247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53110.3431501454721432No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37430.24183976548714592No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC29230.18885857187254276No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT24020.15519613056375223No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA20390.13174226070753153No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA19650.12696103103987222No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG18440.119143074421132No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG17510.11313423173069531No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG16420.10609160965265658No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG15990.10331332754847618No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG15480.10001815575049475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG1400.021.14285928
CTAGCGG1400.021.14285929
ACCGTCC450.003826398920.5555558
CGCAATA1500.019.73333436
GCTTTAT6200.017.9032251
AAGACGG2600.017.0769235
TAGACTC904.4475597E-516.4444455
CTAGACT1251.6586819E-716.2799994
CGAATTA1151.242799E-616.08695615
CGGACCA2650.016.0566029
GAGCGAA2700.015.75925916
CGACGGT1302.5904592E-715.6538467
CTTAAAC2650.015.3584893
TATTGAG7250.015.3103455
GTATACT1101.4524352E-515.1363644
GACGGTA1353.9744373E-715.0740758
ACGGACC2850.014.9298248
GACACGC7850.014.84713430
CAAGACG3250.014.8000014
TACACTC1752.239176E-914.85
Produced by FastQC (version 0.11.5)