##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630634.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1547719
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00096852206376	33.0	31.0	34.0	30.0	34.0
2	32.16394771919192	33.0	31.0	34.0	30.0	34.0
3	32.26724166337688	34.0	31.0	34.0	30.0	34.0
4	35.8566483967697	37.0	35.0	37.0	35.0	37.0
5	35.81562480010906	37.0	35.0	37.0	35.0	37.0
6	35.891444764844266	37.0	35.0	37.0	35.0	37.0
7	35.83795055820856	37.0	35.0	37.0	35.0	37.0
8	35.84557985008907	37.0	35.0	37.0	35.0	37.0
9	37.533686670513184	39.0	37.0	39.0	35.0	39.0
10	37.43744956287285	39.0	37.0	39.0	34.0	39.0
11	37.53756463544093	39.0	37.0	39.0	35.0	39.0
12	37.4811254497748	39.0	37.0	39.0	35.0	39.0
13	37.530875436691026	39.0	37.0	39.0	35.0	39.0
14	38.80474104149397	40.0	38.0	41.0	35.0	41.0
15	38.81844378727663	40.0	38.0	41.0	35.0	41.0
16	38.76957122061563	40.0	38.0	41.0	35.0	41.0
17	38.772810826771526	40.0	38.0	41.0	35.0	41.0
18	38.75980200540279	40.0	38.0	41.0	35.0	41.0
19	38.81294343482247	40.0	38.0	41.0	35.0	41.0
20	38.789976733502655	40.0	38.0	41.0	35.0	41.0
21	38.7412669870952	40.0	38.0	41.0	35.0	41.0
22	38.67525500429988	40.0	38.0	41.0	34.0	41.0
23	38.63523869643004	40.0	38.0	41.0	34.0	41.0
24	38.57505852160502	40.0	38.0	41.0	34.0	41.0
25	38.53141558642105	40.0	38.0	41.0	34.0	41.0
26	38.38730092478027	40.0	38.0	41.0	34.0	41.0
27	38.250643689196814	40.0	38.0	41.0	34.0	41.0
28	38.151968800538086	40.0	38.0	41.0	33.0	41.0
29	38.07644669348893	40.0	38.0	41.0	33.0	41.0
30	37.98965380666645	40.0	37.0	41.0	33.0	41.0
31	37.933670130042984	40.0	37.0	41.0	33.0	41.0
32	37.84530654466347	40.0	37.0	41.0	33.0	41.0
33	37.754784944812336	40.0	37.0	41.0	33.0	41.0
34	37.69129861428334	40.0	37.0	41.0	33.0	41.0
35	37.617097806513975	40.0	37.0	41.0	33.0	41.0
36	37.58224070390038	40.0	37.0	41.0	33.0	41.0
37	37.43542077082468	40.0	37.0	41.0	32.0	41.0
38	37.28479006848142	40.0	36.0	41.0	32.0	41.0
39	37.20726049108398	40.0	36.0	41.0	31.0	41.0
40	37.01670652101576	39.0	36.0	41.0	31.0	41.0
41	36.947098278175815	39.0	36.0	41.0	31.0	41.0
42	36.79304835050807	39.0	35.0	41.0	31.0	41.0
43	35.984560504846165	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	4.0
11	2.0
12	1.0
13	7.0
14	0.0
15	2.0
16	7.0
17	18.0
18	40.0
19	101.0
20	174.0
21	382.0
22	712.0
23	1248.0
24	2162.0
25	3541.0
26	5577.0
27	8240.0
28	11864.0
29	16974.0
30	22568.0
31	29375.0
32	37870.0
33	48798.0
34	64163.0
35	86582.0
36	121459.0
37	191393.0
38	360782.0
39	533667.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.83863026815591	18.858849700753172	11.677895018410965	24.624625012679953
2	19.40914339101607	20.75602871063804	33.32006649785911	26.51476140048678
3	21.531040195280926	23.164023960421755	28.595177806824108	26.709758037473208
4	14.811021897385764	14.697370775961268	33.85931167091701	36.63229565573596
5	14.62552310852293	37.0544007019362	33.78720555863177	14.5328706309091
6	36.042395292685555	33.621994690250624	15.086717937816877	15.248892079246943
7	29.442101570117057	30.090991969472498	21.201458404271058	19.26544805613939
8	28.93050999567751	31.557731086844576	19.696017171075628	19.815741746402285
9	27.203904584746972	13.3528114599614	19.82549803937278	39.617785915918844
10	17.827977817678793	26.710081093531834	32.20222792380271	23.259713164986668
11	35.761401132892985	21.696444897297248	20.958520248184588	21.58363372162518
12	23.066331808293366	25.46754288084594	28.010834007982073	23.45529130287862
13	30.87847341797833	19.49778997350294	25.042271885271163	24.581464723247567
14	23.294344774471334	20.5934022907259	25.366232500860946	30.74602043394182
15	26.317632593513423	27.27497691764461	22.49213196969217	23.91525851914979
16	25.272998522341588	25.821806154734805	24.40287933403932	24.502315988884288
17	24.166789966395708	25.962981652354205	25.359771379688432	24.510457001561655
18	23.62457267759845	24.44513506650755	27.354254874431337	24.576037381462655
19	24.911692626374684	24.68439038352569	27.35729160138242	23.046625388717203
20	25.494033477653243	24.122466675152275	26.45790353416867	23.925596313025814
21	25.21891893812766	24.298403004679788	26.639977928810076	23.842700128382475
22	25.18318893804366	24.65764134187149	26.701100135102045	23.458069584982805
23	24.665653132125406	24.592448629240838	26.322349211969353	24.4195490266644
24	24.049520617114606	25.192040674050002	26.846863028753926	23.911575680081462
25	24.57041620604257	25.10248953459898	26.139564093998974	24.187530165359476
26	24.401974777075168	25.50721416484517	26.30425807268632	23.786552985393346
27	24.56511808668111	24.575843547827482	26.44517512545882	24.41386324003259
28	24.15167094285203	25.21303931786067	26.445110514247094	24.190179225040204
29	24.116845499732186	25.50708494242172	26.995145759663092	23.380923798183005
30	24.193991286531986	25.481046624096493	26.19545279214121	24.12950929723031
31	23.47997278575762	26.07055931987654	26.73611941185706	23.713348482508774
32	23.401534774723316	25.40112255519251	26.47870834434416	24.71863432574001
33	23.008375551375927	25.99393042277054	26.759702504136733	24.237991521716797
34	24.741635917114152	24.88403902775633	26.27557069468037	24.098754360449153
35	23.74946614986312	25.156310673966008	26.703038471453798	24.39118470471707
36	23.203307577150632	25.98921380431461	26.75763494536153	24.049843673173232
37	24.05145895346636	24.656607562483888	27.234724132739856	24.057209351309897
38	23.960034088875307	25.00576655064647	26.64178704273838	24.392412317739847
39	23.64925416047745	25.060621469401102	26.97085194405444	24.319272426067005
40	23.05722162744012	24.728390618710502	27.374930462183382	24.839457291665994
41	22.157510504167746	25.298390728549563	27.869658510362672	24.674440256920022
42	22.972193272809857	24.946259624647627	28.12732802272247	23.954219079820042
43	23.223013996726795	24.678898430529056	27.635249034223914	24.462838538520234
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	137.0
1	168.0
2	199.0
3	621.0
4	1043.0
5	1043.0
6	1488.0
7	1933.0
8	1964.5
9	1996.0
10	2803.5
11	3611.0
12	3611.0
13	6453.0
14	9295.0
15	12563.0
16	15831.0
17	15156.0
18	14481.0
19	14481.0
20	17417.0
21	20353.0
22	18917.5
23	17482.0
24	19603.5
25	21725.0
26	21725.0
27	24310.5
28	26896.0
29	30562.5
30	34229.0
31	38648.5
32	43068.0
33	43068.0
34	48453.5
35	53839.0
36	59065.0
37	64291.0
38	69330.0
39	74369.0
40	74369.0
41	77925.5
42	81482.0
43	83206.0
44	84930.0
45	87678.5
46	90427.0
47	90427.0
48	95445.0
49	100463.0
50	100079.0
51	99695.0
52	100851.0
53	102007.0
54	102007.0
55	98365.5
56	94724.0
57	93641.0
58	92558.0
59	93695.0
60	94832.0
61	94832.0
62	88084.0
63	81336.0
64	75187.0
65	69038.0
66	60662.0
67	52286.0
68	52286.0
69	46909.5
70	41533.0
71	35015.5
72	28498.0
73	21214.5
74	13931.0
75	13931.0
76	10153.5
77	6376.0
78	5060.0
79	3744.0
80	3005.0
81	2266.0
82	2266.0
83	1741.5
84	1217.0
85	1009.5
86	802.0
87	647.0
88	492.0
89	492.0
90	376.0
91	260.0
92	147.0
93	34.0
94	20.5
95	7.0
96	7.0
97	5.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1547719.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.21666417639415
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.33294486710244	49.39683113820152
2	8.36596192842722	9.573448683426381
3	2.0866378736927143	3.581713754304636
4	0.8819666264934181	2.018527531314446
5	0.49278816793982305	1.4097847557556689
6	0.3105451774187601	1.0661015476780762
7	0.22897519098561744	0.917083762514485
8	0.1638121906992681	0.7498229682591567
9	0.11876402133589882	0.6115753012512809
>10	0.8140600789399091	9.03311925350732
>50	0.0995223874357018	4.00682823677036
>100	0.09257694038725885	10.821610069529749
>500	0.00883836468382509	3.445190381219039
>1k	0.002266247354826946	2.1707571528251544
>5k	3.399371032240419E-4	1.1976054634426332
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7021	0.45363531752210834	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6140	0.39671283999227247	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5311	0.3431501454721432	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3743	0.24183976548714592	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2923	0.18885857187254276	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2402	0.15519613056375223	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2039	0.13174226070753153	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1965	0.12696103103987222	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1844	0.119143074421132	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	1751	0.11313423173069531	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	1642	0.10609160965265658	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	1599	0.10331332754847618	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	1548	0.10001815575049475	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.461121172512582E-5	0.0
2	6.461121172512582E-5	0.0	0.0	8.399457524266356E-4	0.0
3	6.461121172512582E-5	0.0	0.0	0.0012922242345025164	0.0
4	6.461121172512582E-5	0.0	0.0	0.0019383363517537745	0.0
5	1.2922242345025163E-4	0.0	0.0	0.0021967811986542776	0.0
6	1.2922242345025163E-4	0.0	0.0	0.0021967811986542776	0.0
7	1.2922242345025163E-4	0.0	0.0	0.0025844484690050328	0.0
8	1.2922242345025163E-4	0.0	0.0	0.0028428933159055357	0.0
9	1.2922242345025163E-4	0.0	0.0	0.00478122966765931	0.0
10	1.2922242345025163E-4	0.0	0.0	0.007365678136664343	0.0
11	1.2922242345025163E-4	0.0	0.0	0.008076401465640726	0.0
12	1.2922242345025163E-4	0.0	0.0	0.008528679947716607	0.0
13	1.2922242345025163E-4	0.0	0.0	0.009239403276692992	0.0
14	1.2922242345025163E-4	0.0	0.0	0.010143960240844753	0.0
15	1.2922242345025163E-4	0.0	0.0	0.011694629322247772	0.0
16	1.2922242345025163E-4	0.0	0.0	0.015506690814030196	0.0
17	1.2922242345025163E-4	0.0	0.0	0.02054636532859001	0.0
18	1.2922242345025163E-4	0.0	0.0	0.02254931289206891	0.0
19	1.2922242345025163E-4	0.0	0.0	0.026102929536950828	0.0
20	1.2922242345025163E-4	0.0	0.0	0.028945822852856365	0.0
21	1.2922242345025163E-4	0.0	0.0	0.03469622069639256	0.0
22	1.2922242345025163E-4	0.0	0.0	0.04490479214896244	0.0
23	1.2922242345025163E-4	0.0	0.0	0.05827931297606349	0.0
24	1.2922242345025163E-4	0.0	0.0	0.07966562405708012	0.0
25	1.2922242345025163E-4	0.0	0.0	0.08522218826544095	0.0
26	1.2922242345025163E-4	0.0	0.0	0.09627070547043746	0.0
27	1.2922242345025163E-4	0.0	0.0	0.10841761327476111	0.0
28	2.5844484690050327E-4	0.0	0.0	0.13380981948273557	0.0
29	2.5844484690050327E-4	0.0	0.0	0.1712197110715834	0.0
30	2.5844484690050327E-4	0.0	0.0	0.2260100186144901	0.0
31	2.5844484690050327E-4	0.0	0.0	0.4044661853992876	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	140	0.0	21.142859	28
CTAGCGG	140	0.0	21.142859	29
ACCGTCC	45	0.0038263989	20.555555	8
CGCAATA	150	0.0	19.733334	36
GCTTTAT	620	0.0	17.903225	1
AAGACGG	260	0.0	17.076923	5
TAGACTC	90	4.4475597E-5	16.444445	5
CTAGACT	125	1.6586819E-7	16.279999	4
CGAATTA	115	1.242799E-6	16.086956	15
CGGACCA	265	0.0	16.056602	9
GAGCGAA	270	0.0	15.759259	16
CGACGGT	130	2.5904592E-7	15.653846	7
CTTAAAC	265	0.0	15.358489	3
TATTGAG	725	0.0	15.310345	5
GTATACT	110	1.4524352E-5	15.136364	4
GACGGTA	135	3.9744373E-7	15.074075	8
ACGGACC	285	0.0	14.929824	8
GACACGC	785	0.0	14.847134	30
CAAGACG	325	0.0	14.800001	4
TACACTC	175	2.239176E-9	14.8	5
>>END_MODULE