FastQCFastQC Report
Fri 10 Feb 2017
ERR1630633.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630633.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847241
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT125441.48057046342186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104071.228339988267801No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT92711.0942577141568928No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61910.7307247878702754No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA32290.38111942174658686No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC27650.3263534224618497No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC26720.3153766165707278No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA22550.26615803531698773No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC21610.25506319925499354No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC19450.22956868234658145No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG18550.2189459669680764No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC17770.20973961364003868No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16940.19994310945763957No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC16770.19793659655281082No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16630.19628417416059893No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC16530.19510387245187613No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC16430.19392357074315336No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.1921531181800692No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG14550.17173389861916505No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT13680.16146527375327682No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG13340.15745224794361934No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT13150.15520967469704605No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC13000.15343922213396188No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC12720.15013437734953808No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC12630.1490721058116876No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG12290.1450590800020301No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG11740.1385674206040548No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT11300.13337409308567455No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11100.13101348966822898No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT11050.1304233388138676No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT10710.12641031300421013No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG10480.12369561907414772No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT10350.12216122685280813RNA PCR Primer, Index 39 (95% over 23bp)
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT10050.11862032172663976No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT9990.11791214070140608No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC9940.1173219898470447No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC9840.11614168813832192No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC9830.11602365796744964No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC9690.11437123557523775No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG9670.11413517523349319No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG9590.11319093386651495No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG8950.10563700293068914No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA8950.10563700293068914No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC8920.10528291241807232No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC8790.10374852019673268No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA8620.10174200729190395No Hit
GCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGC8550.10091579609579802No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGC8550.10091579609579802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAA358.867462E-426.4285721
TACATAC509.087402E-625.9000023
CAATACC551.901894E-523.5454544
TAGAACC400.001930770623.12525
TATATCG400.001930770623.1255
CTACCCG400.001930770623.12510
GTATATG502.7012892E-422.21
CAAACGC603.7247984E-521.58333210
CCAATAC603.7247984E-521.5833323
CCAAACG603.7247984E-521.5833329
GGTATCA44400.021.3751
CTATGGT450.0038249920.5555559
AAACGAT450.0038249920.55555517
GGCTTAA555.141562E-420.18181823
GGTATTG555.141562E-420.18181833
ATGCGAG656.899393E-519.9230777
AGACTAT500.007033391418.56
GTATTAT609.234085E-418.51
CCGTCTT1409.458745E-1118.537
TATACTT500.007033391418.534
Produced by FastQC (version 0.11.5)