##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630633.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	847241
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98661065741625	33.0	31.0	34.0	30.0	34.0
2	32.121305508113984	33.0	31.0	34.0	30.0	34.0
3	32.18141945444094	34.0	31.0	34.0	30.0	34.0
4	35.77723339640079	37.0	35.0	37.0	35.0	37.0
5	35.77343990670895	37.0	35.0	37.0	35.0	37.0
6	35.88736852914342	37.0	35.0	37.0	35.0	37.0
7	35.82853757077384	37.0	35.0	37.0	35.0	37.0
8	35.86204515598277	37.0	35.0	37.0	35.0	37.0
9	37.49546351038252	39.0	37.0	39.0	35.0	39.0
10	37.43579217719633	39.0	37.0	39.0	34.0	39.0
11	37.54791257741304	39.0	37.0	39.0	35.0	39.0
12	37.50798179030524	39.0	37.0	39.0	35.0	39.0
13	37.54830797848546	39.0	37.0	39.0	35.0	39.0
14	38.737801876915775	40.0	38.0	41.0	35.0	41.0
15	38.73830232484028	40.0	38.0	41.0	35.0	41.0
16	38.64503134291188	40.0	38.0	41.0	34.0	41.0
17	38.70960919030122	40.0	38.0	41.0	35.0	41.0
18	38.71681965343981	40.0	38.0	41.0	35.0	41.0
19	38.80928921050799	40.0	38.0	41.0	35.0	41.0
20	38.791232954967946	40.0	38.0	41.0	35.0	41.0
21	38.742939730253845	40.0	38.0	41.0	35.0	41.0
22	38.627382291461345	40.0	38.0	41.0	34.0	41.0
23	38.52469958370759	40.0	38.0	41.0	34.0	41.0
24	38.45745779536165	40.0	38.0	41.0	34.0	41.0
25	38.38041124072135	40.0	38.0	41.0	34.0	41.0
26	38.18435250418712	40.0	37.0	41.0	34.0	41.0
27	37.97724260275412	40.0	37.0	41.0	33.0	41.0
28	37.82376679126718	40.0	37.0	41.0	33.0	41.0
29	37.7261818065934	40.0	37.0	41.0	33.0	41.0
30	37.551150145000065	40.0	36.0	41.0	33.0	41.0
31	37.448624417373566	39.0	36.0	41.0	33.0	41.0
32	37.24698521436049	39.0	35.0	41.0	32.0	41.0
33	37.04949477185358	39.0	35.0	41.0	31.0	41.0
34	36.92469321007836	39.0	35.0	41.0	31.0	41.0
35	36.74992003455923	39.0	35.0	41.0	31.0	41.0
36	36.6562383076362	39.0	35.0	41.0	31.0	41.0
37	36.40923538875007	39.0	35.0	41.0	30.0	41.0
38	36.1582607546141	39.0	35.0	41.0	30.0	41.0
39	35.95111898503495	39.0	35.0	41.0	29.0	41.0
40	35.63252604630796	39.0	35.0	41.0	27.0	41.0
41	35.446041917234886	38.0	35.0	41.0	26.0	41.0
42	35.12230404335956	38.0	35.0	40.0	24.0	41.0
43	34.30240392049016	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	1.0
10	4.0
11	2.0
12	5.0
13	4.0
14	1.0
15	5.0
16	13.0
17	11.0
18	34.0
19	82.0
20	170.0
21	345.0
22	642.0
23	1043.0
24	1794.0
25	2837.0
26	4265.0
27	6208.0
28	8981.0
29	12228.0
30	15968.0
31	19882.0
32	24587.0
33	31727.0
34	40723.0
35	52497.0
36	72937.0
37	118082.0
38	182564.0
39	249597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.212588861964896	19.96338704099542	12.635837972902634	21.18818612413705
2	20.65870277760401	21.08597199616166	32.771194972858964	25.48413025337537
3	23.60886689855661	24.675269492387645	28.061673124884184	23.654190484171565
4	13.9307469775424	15.767296436315052	33.19929040261271	37.102666183529834
5	13.304596921064963	39.71797870971778	31.99455644851937	14.98286792069789
6	33.26267260437113	32.58270078997593	16.593743692762743	17.560882912890193
7	27.696251715863607	32.41592415853341	20.441409233028146	19.44641489257484
8	31.094222305105628	29.099394387193257	19.27656947668963	20.529813831011484
9	28.24190519580615	12.148019276687506	21.853050076660598	37.757025450845745
10	21.696424039913083	26.684497091146437	29.917461501509017	21.701617367431464
11	34.11213574413892	22.42620458641638	19.180256857257852	24.28140281218685
12	23.180771468802856	28.643915957797134	25.921668096798907	22.25364447660111
13	30.53322490294969	19.659931471682793	27.439536094216404	22.367307531151113
14	23.067344474594595	22.03304608724082	27.236642230486957	27.662967207677625
15	27.615755139328712	25.274626700077075	25.600862092368047	21.50875606822616
16	24.850426265962106	24.611769260458356	27.033158215903146	23.504646257676388
17	23.768089599063313	25.93606777764532	26.281896178301096	24.01394644499027
18	22.56087701138165	21.969663885482408	30.289492600098438	25.179966503037505
19	22.47447892630314	24.447117172091527	30.357005857837382	22.721398043767948
20	24.20043411496847	21.871580813487544	31.761682921388367	22.166302150155623
21	24.62994590677269	23.77056823265163	29.822801304469447	21.776684556106233
22	25.842823942656224	22.801894620302843	30.032068797426	21.323212639614937
23	23.7123793584116	22.693660953612962	30.248890221318376	23.34506946665707
24	21.823660564113396	24.90235954114591	30.09061176217865	23.183368132562045
25	22.4827410382642	24.567507946381255	30.302475918894388	22.647275096460156
26	22.633819656980716	25.75772418945731	29.336162909963043	22.272293243598927
27	23.476082956325296	24.468716693361156	30.181140903237687	21.87405944707586
28	21.74292792723676	24.653433910776272	30.324665591018373	23.27897257096859
29	21.374909854456998	24.631244238652283	31.332171129584147	22.661674777306576
30	19.78150254768124	26.63079336339955	31.470148399333837	22.117555689585373
31	22.937629316805964	26.511818951160294	28.46982145576052	22.080730276273222
32	21.352130031478648	25.512693554726457	30.038914547336592	23.096261866458303
33	20.391600500920045	25.477402533635647	29.918169682534252	24.21282728291006
34	22.316200467163416	24.935998139844507	30.727030443521976	22.020770949470105
35	20.435153633971918	26.253096816608263	30.716171667801724	22.595577881618098
36	21.28072177810092	27.27830688080487	29.101636960439826	22.339334380654382
37	21.906399713894867	25.372355681559323	30.116224309257934	22.60502029528788
38	21.032858419269136	25.011065328519276	29.561954626841715	24.394121625369877
39	21.58760022236884	26.078058073204673	29.956175397555125	22.378166306871364
40	20.208299645555396	25.58221332537023	29.998666259069147	24.210820770005228
41	20.45887769831724	25.984460147702954	30.039622728361827	23.517039425617977
42	20.66908943264077	26.55855889882572	30.593184229752808	22.179167438780702
43	19.61649636880179	24.768041206693255	29.63277272936508	25.98268969513987
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	453.0
1	400.0
2	347.0
3	923.5
4	1500.0
5	1500.0
6	2188.5
7	2877.0
8	2936.5
9	2996.0
10	4362.0
11	5728.0
12	5728.0
13	10311.5
14	14895.0
15	19474.5
16	24054.0
17	22197.0
18	20340.0
19	20340.0
20	21469.0
21	22598.0
22	16248.0
23	9898.0
24	8884.5
25	7871.0
26	7871.0
27	8632.0
28	9393.0
29	10796.0
30	12199.0
31	14321.0
32	16443.0
33	16443.0
34	19652.5
35	22862.0
36	25331.0
37	27800.0
38	32444.5
39	37089.0
40	37089.0
41	39652.0
42	42215.0
43	45347.0
44	48479.0
45	49339.5
46	50200.0
47	50200.0
48	55487.5
49	60775.0
50	60587.5
51	60400.0
52	62349.0
53	64298.0
54	64298.0
55	60761.5
56	57225.0
57	52126.0
58	47027.0
59	43964.0
60	40901.0
61	40901.0
62	39280.0
63	37659.0
64	34437.5
65	31216.0
66	29801.0
67	28386.0
68	28386.0
69	22273.0
70	16160.0
71	13805.0
72	11450.0
73	7977.5
74	4505.0
75	4505.0
76	3469.0
77	2433.0
78	1885.0
79	1337.0
80	1100.5
81	864.0
82	864.0
83	828.0
84	792.0
85	788.5
86	785.0
87	615.0
88	445.0
89	445.0
90	350.5
91	256.0
92	166.5
93	77.0
94	43.0
95	9.0
96	9.0
97	6.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	847241.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.70481005157811
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.07177493710437	31.322054946509663
2	8.770475554154809	6.613782296628326
3	2.4753095828984577	2.799932329261121
4	1.1589161596796045	1.7478685466569541
5	0.7355125201657945	1.3866179931704394
6	0.5130901584438825	1.160758017807642
7	0.3788817876449195	0.9999966084607826
8	0.28670491540922927	0.8648123501087007
9	0.2352161159063611	0.7981901074187392
>10	1.876448373173136	14.460867634910224
>50	0.2590524334389732	6.778234019141673
>100	0.20529275714528486	14.826482581090136
>500	0.02263564856732069	6.0172205246429815
>1k	0.009431520236383622	5.6697859602497624
>5k	6.287680157589081E-4	1.832832901619908
>10k+	6.287680157589081E-4	2.7205631823230187
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12544	1.48057046342186	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10407	1.228339988267801	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9271	1.0942577141568928	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6191	0.7307247878702754	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	3229	0.38111942174658686	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	2765	0.3263534224618497	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2672	0.3153766165707278	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	2255	0.26615803531698773	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	2161	0.25506319925499354	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	1945	0.22956868234658145	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1855	0.2189459669680764	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1777	0.20973961364003868	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1694	0.19994310945763957	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1677	0.19793659655281082	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1663	0.19628417416059893	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1653	0.19510387245187613	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	1643	0.19392357074315336	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1628	0.1921531181800692	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	1455	0.17173389861916505	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	1368	0.16146527375327682	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	1334	0.15745224794361934	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	1315	0.15520967469704605	No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC	1300	0.15343922213396188	No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCAC	1272	0.15013437734953808	No Hit
ACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATC	1263	0.1490721058116876	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	1229	0.1450590800020301	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	1174	0.1385674206040548	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT	1130	0.13337409308567455	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1110	0.13101348966822898	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	1105	0.1304233388138676	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	1071	0.12641031300421013	No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGG	1048	0.12369561907414772	No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT	1035	0.12216122685280813	RNA PCR Primer, Index 39 (95% over 23bp)
ACGCAGAGTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTT	1005	0.11862032172663976	No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCT	999	0.11791214070140608	No Hit
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACAC	994	0.1173219898470447	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGC	984	0.11614168813832192	No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC	983	0.11602365796744964	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	969	0.11437123557523775	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	967	0.11413517523349319	No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGG	959	0.11319093386651495	No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAG	895	0.10563700293068914	No Hit
GCGTGTGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGA	895	0.10563700293068914	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	892	0.10528291241807232	No Hit
GCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGC	879	0.10374852019673268	No Hit
GTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGA	862	0.10174200729190395	No Hit
GCGTTCAGCCACCCGAGATTGAGCAATAACAGGTCTGTGATGC	855	0.10091579609579802	No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGC	855	0.10091579609579802	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.721206834891135E-4	0.0
3	0.0	0.0	0.0	0.0012983318795950622	0.0
4	0.0	0.0	0.0	0.0025966637591901243	0.0
5	0.0	0.0	0.0	0.002950754271806959	0.0
6	0.0	0.0	0.0	0.002950754271806959	0.0
7	0.0	0.0	0.0	0.0030687844426792376	0.0
8	0.0	0.0	0.0	0.003186814613551516	0.0
9	0.0	0.0	0.0	0.005193327518380249	0.0
10	0.0	0.0	0.0	0.006845749910592146	0.0
11	0.0	0.0	0.0	0.00861620247367632	0.0
12	0.0	1.1803017087227837E-4	0.0	0.009560443840654548	0.0
13	0.0	1.1803017087227837E-4	0.0	0.010622715378505053	0.0
14	0.0	1.1803017087227837E-4	0.0	0.012039077428972395	0.0
15	0.0	1.1803017087227837E-4	0.0	0.015579982555140745	0.0
16	0.0	1.1803017087227837E-4	0.0	0.02537648673753985	0.0
17	0.0	1.1803017087227837E-4	0.0	0.03906798655872414	0.0
18	0.0	1.1803017087227837E-4	0.0	0.04461540458972123	0.0
19	0.0	1.1803017087227837E-4	0.0	0.05429387860124805	0.0
20	0.0	1.1803017087227837E-4	0.0	0.060549477657478805	0.0
21	0.0	1.1803017087227837E-4	0.0	0.07447703782040765	0.0
22	0.0	1.1803017087227837E-4	0.0	0.09961746421620295	0.0
23	0.0	2.3606034174455674E-4	0.0	0.13962969214190532	0.0
24	0.0	2.3606034174455674E-4	0.0	0.19604811381885437	0.0
25	0.0	2.3606034174455674E-4	0.0	0.2122182472283565	0.0
26	0.0	2.3606034174455674E-4	0.0	0.2368865529406627	0.0
27	0.0	2.3606034174455674E-4	0.0	0.26356137155779763	0.0
28	0.0	2.3606034174455674E-4	0.0	0.31242586229892083	0.0
29	0.0	2.3606034174455674E-4	0.0	0.38678486994845623	0.0
30	0.0	2.3606034174455674E-4	0.0	0.509654277826498	0.0
31	0.0	2.3606034174455674E-4	0.0	0.8659873636899064	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAAA	35	8.867462E-4	26.428572	1
TACATAC	50	9.087402E-6	25.900002	3
CAATACC	55	1.901894E-5	23.545454	4
TAGAACC	40	0.0019307706	23.125	25
TATATCG	40	0.0019307706	23.125	5
CTACCCG	40	0.0019307706	23.125	10
GTATATG	50	2.7012892E-4	22.2	1
CAAACGC	60	3.7247984E-5	21.583332	10
CCAATAC	60	3.7247984E-5	21.583332	3
CCAAACG	60	3.7247984E-5	21.583332	9
GGTATCA	4440	0.0	21.375	1
CTATGGT	45	0.00382499	20.555555	9
AAACGAT	45	0.00382499	20.555555	17
GGCTTAA	55	5.141562E-4	20.181818	23
GGTATTG	55	5.141562E-4	20.181818	33
ATGCGAG	65	6.899393E-5	19.923077	7
AGACTAT	50	0.0070333914	18.5	6
GTATTAT	60	9.234085E-4	18.5	1
CCGTCTT	140	9.458745E-11	18.5	37
TATACTT	50	0.0070333914	18.5	34
>>END_MODULE