FastQCFastQC Report
Fri 10 Feb 2017
ERR1630632.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630632.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455424
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72761.5976320966835302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70581.5497646149522204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58741.2897870994940976No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32010.7028615092748736No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10910.2395569842608207No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.17851496627318716No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8030.17631921023046657No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.16643830803822374No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA6390.14030881112984825No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT6050.13284324058459807No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5930.13020833333333331No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5880.12911045531197302No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5770.1266951236649803No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA5580.12252318718381112No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5430.11922955311973017No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT5140.11286186059584036No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5020.1102269533445756No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4960.10890949971894323No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT4820.10583544125913436RNA PCR Primer, Index 47 (95% over 22bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC4820.10583544125913436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAATG405.9323756E-527.752
GTGCGCT502.6986326E-422.19999914
TGCGCTA450.00382231420.55555715
ATCGGGT450.00382231420.55555727
TGTAATA450.00382231420.5555572
GTATATA555.136541E-420.1818181
AGTACTC555.136541E-420.1818185
TAATACT801.61318E-518.54
GTCACTA801.61318E-518.521
CTTTGCG701.2171473E-418.59
CGTCACT801.61318E-518.520
GATCACA500.007028521518.4999981
AGTGCGC500.007028521518.49999813
GCGGGGT500.007028521518.49999829
GTAGTCC500.007028521518.4999986
TGCCGAT500.007028521518.49999822
GCGTTAT953.5967205E-617.5263161
CATAGTA852.7172735E-517.4117643
TCACTAC852.7172735E-517.41176422
TTTCGGA1603.274181E-1117.3437530
Produced by FastQC (version 0.11.5)