##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630632.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	455424
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.553675256464306	31.0	31.0	34.0	30.0	34.0
2	31.68038794617763	31.0	31.0	34.0	30.0	34.0
3	31.712885135609895	31.0	31.0	34.0	30.0	34.0
4	35.445516705311974	37.0	35.0	37.0	33.0	37.0
5	35.386543089516586	37.0	35.0	37.0	33.0	37.0
6	35.493500562113546	37.0	35.0	37.0	33.0	37.0
7	35.41731441469927	37.0	35.0	37.0	33.0	37.0
8	35.46205074831366	37.0	35.0	37.0	33.0	37.0
9	37.01562280424396	39.0	37.0	39.0	33.0	39.0
10	36.86907585019674	39.0	37.0	39.0	33.0	39.0
11	37.02627880831928	39.0	37.0	39.0	33.0	39.0
12	36.9613898257448	39.0	37.0	39.0	33.0	39.0
13	37.02004944842608	39.0	37.0	39.0	33.0	39.0
14	38.078546585160204	40.0	37.0	41.0	33.0	41.0
15	38.027866339938164	40.0	37.0	41.0	33.0	41.0
16	37.93430956646993	40.0	37.0	41.0	33.0	41.0
17	37.9862150435638	40.0	37.0	41.0	33.0	41.0
18	38.03865408937605	40.0	37.0	41.0	33.0	41.0
19	38.137963743676224	40.0	37.0	41.0	33.0	41.0
20	38.124213919336704	40.0	38.0	41.0	33.0	41.0
21	38.08007922287802	40.0	37.0	41.0	33.0	41.0
22	37.95354219364812	40.0	37.0	41.0	33.0	41.0
23	37.85946502599775	40.0	37.0	41.0	33.0	41.0
24	37.75375913434514	40.0	37.0	41.0	33.0	41.0
25	37.6721933846262	39.0	37.0	41.0	32.0	41.0
26	37.443779862282184	39.0	36.0	41.0	32.0	41.0
27	37.23619747751546	39.0	36.0	41.0	32.0	41.0
28	37.053056053260256	39.0	35.0	40.0	31.0	41.0
29	36.86407172217538	39.0	35.0	40.0	31.0	41.0
30	36.6642886628724	39.0	35.0	40.0	31.0	41.0
31	36.513409482152895	38.0	35.0	40.0	30.0	41.0
32	36.290595137717816	38.0	35.0	40.0	30.0	41.0
33	36.07606538083193	38.0	35.0	40.0	30.0	41.0
34	35.92659367973581	38.0	35.0	40.0	30.0	41.0
35	35.739971982152895	38.0	35.0	40.0	29.0	41.0
36	35.58357925801012	38.0	35.0	40.0	28.0	41.0
37	35.30344470207982	38.0	34.0	40.0	27.0	41.0
38	34.99624306141091	38.0	34.0	40.0	25.0	41.0
39	34.775046989179316	38.0	34.0	40.0	24.0	41.0
40	34.40193973088814	37.0	33.0	40.0	23.0	41.0
41	34.1553453485104	37.0	33.0	40.0	21.0	41.0
42	33.81498998735245	37.0	33.0	40.0	20.0	41.0
43	32.91200946810006	36.0	32.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	4.0
15	2.0
16	10.0
17	21.0
18	32.0
19	93.0
20	166.0
21	350.0
22	561.0
23	940.0
24	1571.0
25	2438.0
26	3523.0
27	5028.0
28	7012.0
29	9091.0
30	11190.0
31	14025.0
32	16991.0
33	21194.0
34	27172.0
35	35305.0
36	50157.0
37	77323.0
38	106591.0
39	64633.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.02129005059022	19.782224915682967	13.195615514333895	21.000869519392918
2	19.193103569421023	23.318490022484543	33.19653773187184	24.2918686762226
3	22.1909692945475	26.612124086565487	29.17259520798201	22.024311410905003
4	13.912749437886454	16.556000562113546	32.98574515177066	36.54550484822934
5	12.97801609050028	40.353824128724	31.750632377740303	14.917527403035413
6	32.513657602585724	32.99891968802699	17.102963392355257	17.384459317032043
7	26.908770727937043	33.05513104272063	21.665744449128724	18.370353780213602
8	30.528913715570543	30.095032321528947	19.91572688308038	19.460327079820125
9	28.028825885328835	12.272519673974143	21.598115163012928	38.10053927768409
10	19.29366919617763	27.793440837549184	29.14119589657111	23.77169406970208
11	33.18797428330523	23.51347315907813	20.072503864530635	23.226048693086003
12	22.298780916245082	29.005717748735243	27.6054841202923	21.090017214727375
13	30.21360314783586	19.676389474423832	27.608338603147835	22.501668774592467
14	23.616893268690276	22.00059724564362	27.784877388982576	26.597632096683533
15	27.575402262507026	25.593073707138842	25.913434513771783	20.91808951658235
16	23.95503969926925	25.420267706576727	27.915085722315908	22.709606871838112
17	21.42091765036537	27.6353464024733	27.952852726250704	22.990883220910625
18	21.051811059584036	22.98188062113547	32.05606204328274	23.91024627599775
19	21.843381112984822	25.883352655986506	31.898845910623947	20.37442032040472
20	22.909201096121418	22.616287240022483	32.95588286958966	21.51862879426644
21	24.940055860033727	23.757641231028668	31.035694210230467	20.266608698707138
22	23.813633010118043	24.015203414839796	30.576122470489036	21.59504110455312
23	21.926819842608207	24.600152824620576	31.34880902192243	22.124218310848793
24	21.22769111860596	26.27814959246768	30.98167861157954	21.512480677346822
25	21.505234682405845	25.42663539910062	30.97048025576166	22.09764966273187
26	22.07503337549185	25.883133080382237	31.05479728780214	20.987036256323776
27	22.015967537942664	25.499534499718944	30.68459281899944	21.799905143338954
28	20.928189994378865	25.562772273749296	32.52441680719505	20.984620924676783
29	20.49145411748173	26.456884134345138	32.37883818156268	20.672823566610454
30	19.97720805227656	27.259213392355257	32.69985771500843	20.063720840359753
31	20.740013701517707	27.75589340921866	30.525620081506467	20.978472807757168
32	19.582411115795388	27.429604061270375	31.05545601461495	21.93252880831928
33	19.80923271500843	26.948294336706013	31.787521079258006	21.454951869027543
34	20.82345243114109	27.294345489038786	30.624868254637438	21.257333825182688
35	20.567866427768408	27.88236895727937	30.59983663575042	20.9499279792018
36	19.780029159640247	28.735200604272066	29.989416455874085	21.495353780213602
37	20.367174325463743	28.388051573917934	29.92310462338392	21.3216694772344
38	20.01212057335582	27.139105536818438	30.935567734682408	21.91320615514334
39	20.70092924395728	27.61821950534008	30.1756165682968	21.505234682405845
40	19.940758501967398	28.019384134345138	30.376089094997187	21.663768268690276
41	19.043572582911747	28.489715078695898	30.659122048903882	21.807590289488477
42	20.034956436200112	28.33162064362001	30.59105361157954	21.042369308600335
43	19.223844154019112	27.9677838673412	30.168150997751546	22.64022098088814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	46.0
2	78.0
3	413.5
4	749.0
5	749.0
6	1241.5
7	1734.0
8	1919.5
9	2105.0
10	2736.0
11	3367.0
12	3367.0
13	5804.5
14	8242.0
15	12343.5
16	16445.0
17	14979.0
18	13513.0
19	13513.0
20	13996.0
21	14479.0
22	10552.5
23	6626.0
24	6615.5
25	6605.0
26	6605.0
27	7311.5
28	8018.0
29	8928.0
30	9838.0
31	10833.0
32	11828.0
33	11828.0
34	13177.5
35	14527.0
36	16046.5
37	17566.0
38	19935.0
39	22304.0
40	22304.0
41	23509.0
42	24714.0
43	25811.0
44	26908.0
45	27614.0
46	28320.0
47	28320.0
48	30380.0
49	32440.0
50	30851.0
51	29262.0
52	27328.5
53	25395.0
54	25395.0
55	24030.0
56	22665.0
57	22243.0
58	21821.0
59	20962.5
60	20104.0
61	20104.0
62	18621.5
63	17139.0
64	15737.5
65	14336.0
66	12792.5
67	11249.0
68	11249.0
69	9777.0
70	8305.0
71	7292.5
72	6280.0
73	4791.5
74	3303.0
75	3303.0
76	2627.0
77	1951.0
78	1568.5
79	1186.0
80	983.0
81	780.0
82	780.0
83	614.5
84	449.0
85	395.0
86	341.0
87	307.0
88	273.0
89	273.0
90	206.0
91	139.0
92	79.0
93	19.0
94	12.5
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	455424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.76549849979913
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.08000597054055	41.48968903519295
2	8.514206768142836	8.30399074757698
3	2.0843938960509285	3.0493952223258605
4	0.969738577950864	1.891591406730408
5	0.5597476889741027	1.3648187543466315
6	0.38734608555402716	1.1333474972392785
7	0.26782363994869385	0.914238731849014
8	0.19811039017019338	0.7728761547711348
9	0.1780417260162015	0.7814064170650248
>10	1.3945589088416588	13.920673940045353
>50	0.21283754298583837	7.245557487872942
>100	0.14550677036392506	12.082319692896881
>500	0.005422612560146275	1.651184983473642
>1k	9.037687600243794E-4	0.9458008740249397
>5k	0.0013556531400365688	4.453109054588998
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7276	1.5976320966835302	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7058	1.5497646149522204	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5874	1.2897870994940976	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3201	0.7028615092748736	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1091	0.2395569842608207	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	813	0.17851496627318716	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	803	0.17631921023046657	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	758	0.16643830803822374	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	639	0.14030881112984825	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	605	0.13284324058459807	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	593	0.13020833333333331	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	588	0.12911045531197302	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	577	0.1266951236649803	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	558	0.12252318718381112	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	543	0.11922955311973017	No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	514	0.11286186059584036	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	502	0.1102269533445756	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	496	0.10890949971894323	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	482	0.10583544125913436	RNA PCR Primer, Index 47 (95% over 22bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	482	0.10583544125913436	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.391512085441259E-4	0.0
2	0.0	0.0	0.0	0.003952360876897133	0.0
3	0.0	0.0	0.0	0.0070264193367060145	0.0
4	0.0	0.0	0.0	0.011417931422147273	0.0
5	0.0	0.0	0.0	0.012735385047779652	0.0
6	0.0	0.0	0.0	0.01339411186059584	0.0
7	0.0	0.0	0.0	0.015150716694772344	0.0
8	0.0	0.0	0.0	0.01624859471613266	0.0
9	0.0	0.0	0.0	0.022835862844294546	0.0
10	0.0	2.1957560427206295E-4	0.0	0.03052100899381675	0.0
11	0.0	2.1957560427206295E-4	0.0	0.03403421866216976	0.0
12	0.0	2.1957560427206295E-4	0.0	0.03754742833052276	0.0
13	0.0	2.1957560427206295E-4	0.0	0.04018233558178752	0.0
14	0.0	2.1957560427206295E-4	0.0	0.04391512085441259	0.0
15	0.0	2.1957560427206295E-4	0.0	0.05094154019111861	0.0
16	0.0	2.1957560427206295E-4	0.0	0.06411607644744238	0.0
17	0.0	4.391512085441259E-4	0.0	0.0770710370994941	0.0
18	0.0	4.391512085441259E-4	0.0	0.08431703204047218	0.0
19	0.0	4.391512085441259E-4	0.0	0.09595453906689151	0.0
20	0.0	4.391512085441259E-4	0.0	0.10649416807195053	0.0
21	0.0	4.391512085441259E-4	0.0	0.1304279089376054	0.0
22	0.0	4.391512085441259E-4	0.0	0.17104939572793704	0.0
23	0.0	4.391512085441259E-4	0.0	0.24153316469926925	0.0
24	0.0	4.391512085441259E-4	0.0	0.3271676503653738	0.0
25	0.0	4.391512085441259E-4	0.0	0.3515405424395728	0.0
26	0.0	4.391512085441259E-4	0.0	0.3895271219786397	0.0
27	0.0	4.391512085441259E-4	0.0	0.4125825604272063	0.0
28	0.0	4.391512085441259E-4	0.0	0.44617762788083193	0.0
29	0.0	4.391512085441259E-4	0.0	0.4907514755480607	0.0
30	0.0	4.391512085441259E-4	0.0	0.5761663856098932	0.0
31	0.0	4.391512085441259E-4	0.0	0.8400962619449128	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAATG	40	5.9323756E-5	27.75	2
GTGCGCT	50	2.6986326E-4	22.199999	14
TGCGCTA	45	0.003822314	20.555557	15
ATCGGGT	45	0.003822314	20.555557	27
TGTAATA	45	0.003822314	20.555557	2
GTATATA	55	5.136541E-4	20.181818	1
AGTACTC	55	5.136541E-4	20.181818	5
TAATACT	80	1.61318E-5	18.5	4
GTCACTA	80	1.61318E-5	18.5	21
CTTTGCG	70	1.2171473E-4	18.5	9
CGTCACT	80	1.61318E-5	18.5	20
GATCACA	50	0.0070285215	18.499998	1
AGTGCGC	50	0.0070285215	18.499998	13
GCGGGGT	50	0.0070285215	18.499998	29
GTAGTCC	50	0.0070285215	18.499998	6
TGCCGAT	50	0.0070285215	18.499998	22
GCGTTAT	95	3.5967205E-6	17.526316	1
CATAGTA	85	2.7172735E-5	17.411764	3
TCACTAC	85	2.7172735E-5	17.411764	22
TTTCGGA	160	3.274181E-11	17.34375	30
>>END_MODULE