FastQCFastQC Report
Fri 10 Feb 2017
ERR1630631.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630631.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences580911
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT91501.5751121944669666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80971.393845184546342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72961.2559583137520207No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29630.5100609215525269No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA20850.35891900824739076No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20330.3499675509673599No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG14760.254083672025491No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC13640.2348036101915784No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA13310.22912287768694345No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC12310.2119085367638072No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC10510.18092272310216195No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC10390.1788570021913856No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.17317626968675065No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC9950.17128269218520564No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC9740.16766768059134704No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC9370.16129837444978662No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC9360.16112623104055526No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG7830.13478828942815682No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG7440.12807469646813366No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC7430.1279025530589023No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC7320.12600897555735732No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT7230.12445968487427507No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC7210.12411539805581234No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC7020.12084467328041645No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.11963966941579691No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6940.11946752600656554No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG6920.11912323918810283No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC6890.11860680896040873No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG6780.11671323145886374No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG6310.10862249122498971No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG6260.1077617741788329No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT6130.10552390985882519No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.10431890599420565No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT5860.1008760378095784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT308.304905E-637.036
CGCCGAA303.597306E-430.83333433
TCTTGCG250.005494132629.62
AGGACGG405.9351478E-527.7500025
GCCGAAT358.864093E-426.4285734
ACGGATC509.080983E-625.9000028
GGACGGA509.080983E-625.9000026
GCAGCGT659.3750714E-825.6153851
CAGCGTC601.3355038E-624.6666682
AGCGTCA601.3355038E-624.6666683
GTCTATT451.3221118E-424.6666662
TAGGACG451.3221118E-424.6666664
TACACTT451.3221118E-424.6666665
AGACGAA551.9005594E-523.54545415
CTTATCC551.9005594E-523.5454543
TATACTG551.9005594E-523.5454545
GGTATCA28150.023.4618111
CATGCGC400.001930043123.12500229
GCAGTCG802.7199349E-823.1250029
GCGCCGA400.001930043123.12500232
Produced by FastQC (version 0.11.5)