##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630631.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	580911
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02847940562324	33.0	31.0	34.0	30.0	34.0
2	32.14833253286648	33.0	31.0	34.0	30.0	34.0
3	32.22842225401137	34.0	31.0	34.0	30.0	34.0
4	35.82713530988396	37.0	35.0	37.0	35.0	37.0
5	35.825907927376136	37.0	35.0	37.0	35.0	37.0
6	35.93672352563473	37.0	35.0	37.0	35.0	37.0
7	35.90574287627537	37.0	35.0	37.0	35.0	37.0
8	35.919602142152584	37.0	35.0	37.0	35.0	37.0
9	37.59305986631343	39.0	37.0	39.0	35.0	39.0
10	37.498126218990514	39.0	37.0	39.0	35.0	39.0
11	37.61965258017149	39.0	37.0	39.0	35.0	39.0
12	37.55992914577276	39.0	37.0	39.0	35.0	39.0
13	37.61616323326637	39.0	37.0	39.0	35.0	39.0
14	38.79498408534182	40.0	38.0	41.0	35.0	41.0
15	38.7997128647934	40.0	38.0	41.0	35.0	41.0
16	38.749946205184614	40.0	38.0	41.0	35.0	41.0
17	38.81757790780343	40.0	38.0	41.0	35.0	41.0
18	38.849579367579544	40.0	38.0	41.0	35.0	41.0
19	38.94622412039021	40.0	38.0	41.0	35.0	41.0
20	38.911769617032554	40.0	38.0	41.0	35.0	41.0
21	38.86556804742895	40.0	38.0	41.0	35.0	41.0
22	38.765206718412976	40.0	38.0	41.0	35.0	41.0
23	38.68501026835436	40.0	38.0	41.0	35.0	41.0
24	38.628147857417055	40.0	38.0	41.0	34.0	41.0
25	38.559892995656824	40.0	38.0	41.0	34.0	41.0
26	38.41032447311206	40.0	38.0	41.0	34.0	41.0
27	38.250559896438524	40.0	38.0	41.0	34.0	41.0
28	38.14321987361231	40.0	37.0	41.0	34.0	41.0
29	38.051042242271194	40.0	37.0	41.0	33.0	41.0
30	37.894563883279886	40.0	37.0	41.0	33.0	41.0
31	37.78113686950324	40.0	37.0	41.0	33.0	41.0
32	37.596843578448336	40.0	36.0	41.0	33.0	41.0
33	37.44696519776696	40.0	36.0	41.0	33.0	41.0
34	37.335636612148846	40.0	35.0	41.0	33.0	41.0
35	37.189201099652095	40.0	35.0	41.0	32.0	41.0
36	37.1113974429818	40.0	35.0	41.0	32.0	41.0
37	36.91530716409226	39.0	35.0	41.0	31.0	41.0
38	36.709836790833705	39.0	35.0	41.0	31.0	41.0
39	36.57646868453171	39.0	35.0	41.0	30.0	41.0
40	36.34061327810973	39.0	35.0	41.0	30.0	41.0
41	36.20895283442731	39.0	35.0	41.0	30.0	41.0
42	35.9338642236074	39.0	35.0	41.0	28.0	41.0
43	35.16815656787357	38.0	35.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	3.0
10	6.0
11	1.0
12	2.0
13	3.0
14	3.0
15	2.0
16	3.0
17	12.0
18	22.0
19	28.0
20	80.0
21	158.0
22	305.0
23	525.0
24	947.0
25	1476.0
26	2283.0
27	3482.0
28	5183.0
29	7352.0
30	9606.0
31	12046.0
32	15053.0
33	19021.0
34	25095.0
35	32613.0
36	46726.0
37	80012.0
38	127394.0
39	191468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.76015603078613	19.561516308005874	12.45543637493523	20.222891286272766
2	19.221533074773934	21.740163295237995	35.97607895185321	23.06222467813486
3	21.653919447213084	26.483747080017423	29.12477126444498	22.737562208324512
4	11.773576330969803	15.510637601973452	35.836298503557344	36.8794875634994
5	11.75154197458819	41.718438796993	33.00299013101835	13.527029097400462
6	31.59038131486579	35.836298503557344	17.118112757375915	15.455207424200953
7	27.5424290467903	32.63976753754017	20.768413750126953	19.04938966554257
8	30.677504815711877	30.952073553435895	19.299341895746508	19.071079735105723
9	28.13494666136465	11.926956108594949	21.39553218995681	38.542565040083595
10	19.345992759648208	28.011175550127298	31.705717399050805	20.937114291173692
11	34.394769594653916	23.207685858935363	19.70353462062175	22.694009925788976
12	22.47865852084054	29.847085009579782	27.689267374864652	19.984989094715026
13	30.976001487319056	20.330825203860833	27.156139236475124	21.53703407234499
14	22.29412078614452	21.73035112091181	28.729529996849774	27.245998096093892
15	25.868850822242994	27.131006298727346	26.099867277431482	20.900275601598178
16	23.623756479047564	26.442432661801895	26.45052340203577	23.483287457114773
17	21.75049189979188	27.26458958429088	27.285246793398642	23.699671722518595
18	21.564577017822007	23.84014074445139	31.339051937388003	23.256230300338608
19	22.21958268994734	25.110386961169613	31.180335714076683	21.489694634806362
20	24.077354362372205	23.03674745356862	32.13332162758151	20.75257655647767
21	24.3687931542009	24.77866661158078	30.072420732263634	20.78011950195469
22	24.819808886387072	23.976822611381092	30.866173992229445	20.337194510002394
23	22.944306442811378	23.768356942801912	30.772183690789124	22.51515292359759
24	21.244734563470136	26.087817238785288	30.41205967867711	22.255388519067466
25	21.885796619447728	25.58188775905431	30.820211701964674	21.712103919533284
26	22.083933683473028	26.476689200238933	30.24215413376576	21.19722298252228
27	22.30789225888303	25.561058406537317	31.05570388579318	21.075345448786475
28	20.900792031825873	25.463797380321594	31.210116523873708	22.42529406397882
29	20.969305108699956	25.021388818596996	32.40203748939166	21.607268583311384
30	19.70904320971715	27.123948418948856	31.55009975710565	21.616908614228343
31	22.301867239559932	27.22293087925689	29.261453131374687	21.213748749808488
32	20.472154942839783	26.05562642125902	30.981682219823693	22.490536416077507
33	20.028713520659792	25.842857167449058	31.0333252425931	23.09510406929805
34	21.838112895090642	25.340714842721173	31.670944430386065	21.15022783180212
35	20.066068640462998	26.869004029877207	31.169146392476645	21.895780937183147
36	20.896832733413554	27.886543721843793	29.80542630454579	21.411197240196863
37	21.126816328146653	26.275109268029013	30.509664991711293	22.088409412113045
38	20.60246750362792	26.12620521904388	29.924721687143123	23.34660559018507
39	21.061229689229503	26.935279242431285	30.245597001950387	21.75789406638883
40	19.90339311873936	26.578942385322364	30.44029119779106	23.077373298147222
41	19.883940913496215	26.73249430635674	30.62104177748399	22.76252300266306
42	20.029918524524412	27.113791957804207	31.422885777683675	21.433403739987707
43	19.474411742934805	25.29991685473334	30.224251219205694	25.00142018312616
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	95.0
1	107.0
2	119.0
3	516.5
4	914.0
5	914.0
6	1401.5
7	1889.0
8	1883.5
9	1878.0
10	2588.5
11	3299.0
12	3299.0
13	5567.0
14	7835.0
15	12482.5
16	17130.0
17	15607.0
18	14084.0
19	14084.0
20	14616.0
21	15148.0
22	11202.0
23	7256.0
24	7235.0
25	7214.0
26	7214.0
27	8127.0
28	9040.0
29	10325.0
30	11610.0
31	13571.0
32	15532.0
33	15532.0
34	18367.5
35	21203.0
36	23552.5
37	25902.0
38	28667.0
39	31432.0
40	31432.0
41	32711.0
42	33990.0
43	34612.5
44	35235.0
45	35723.0
46	36211.0
47	36211.0
48	37772.0
49	39333.0
50	39253.5
51	39174.0
52	40969.5
53	42765.0
54	42765.0
55	40942.5
56	39120.0
57	35020.5
58	30921.0
59	28449.5
60	25978.0
61	25978.0
62	23540.0
63	21102.0
64	18338.5
65	15575.0
66	14765.0
67	13955.0
68	13955.0
69	10447.5
70	6940.0
71	5825.0
72	4710.0
73	3147.0
74	1584.0
75	1584.0
76	1238.5
77	893.0
78	686.5
79	480.0
80	417.0
81	354.0
82	354.0
83	388.5
84	423.0
85	361.5
86	300.0
87	236.5
88	173.0
89	173.0
90	132.5
91	92.0
92	54.0
93	16.0
94	8.5
95	1.0
96	1.0
97	3.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	580911.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.93284122160489
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.06631881013264	46.77399834181063
2	5.298618655959069	5.503446427075116
3	1.2964034397722295	2.0197774199050085
4	0.6700170298519202	1.3918355210828428
5	0.42884147356766567	1.1135478078014323
6	0.29302799266883856	0.9130665730053836
7	0.22188546704583925	0.8066199910631247
8	0.18088820830472235	0.7515230880599789
9	0.141058704590652	0.6593021377588439
>10	1.1137011775585786	11.580730464879517
>50	0.15743390830018325	5.672546913977586
>100	0.11582875258129222	11.889756493269106
>500	0.01164944350673916	3.988604071849744
>1k	0.003328412430496903	2.6928911304592487
>5k	9.985237291490708E-4	4.242353618002525
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9150	1.5751121944669666	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8097	1.393845184546342	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7296	1.2559583137520207	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2963	0.5100609215525269	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	2085	0.35891900824739076	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	2033	0.3499675509673599	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG	1476	0.254083672025491	No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC	1364	0.2348036101915784	No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA	1331	0.22912287768694345	No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC	1231	0.2119085367638072	No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC	1051	0.18092272310216195	No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC	1039	0.1788570021913856	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1006	0.17317626968675065	No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC	995	0.17128269218520564	No Hit
GTACATGGGCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCAC	974	0.16766768059134704	No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	937	0.16129837444978662	No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC	936	0.16112623104055526	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTG	783	0.13478828942815682	No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG	744	0.12807469646813366	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	743	0.1279025530589023	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC	732	0.12600897555735732	No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT	723	0.12445968487427507	No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC	721	0.12411539805581234	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	702	0.12084467328041645	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	695	0.11963966941579691	No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC	694	0.11946752600656554	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	692	0.11912323918810283	No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC	689	0.11860680896040873	No Hit
GTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG	678	0.11671323145886374	No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG	631	0.10862249122498971	No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG	626	0.1077617741788329	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	613	0.10552390985882519	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	606	0.10431890599420565	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAAT	586	0.1008760378095784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0027542945477017993	0.0
3	0.0	0.0	0.0	0.003959298412321337	0.0
4	0.0	0.0	0.0	0.007402166596948586	0.0
5	0.0	0.0	0.0	0.008951457280030849	0.0
6	0.0	0.0	0.0	0.00912360068926221	0.0
7	0.0	0.0	0.0	0.01050074796311311	0.0
8	0.0	0.0	0.0	0.01118932160003856	0.0
9	0.0	0.0	0.0	0.016870054104673522	0.0
10	0.0	0.0	0.0	0.02599365479393573	0.0
11	0.0	0.0	0.0	0.028920092750868893	0.0
12	0.0	0.0	0.0	0.03201867411703342	0.0
13	0.0	0.0	0.0	0.03356796480011568	0.0
14	0.0	0.0	0.0	0.03494511207396658	0.0
15	0.0	0.0	0.0	0.03838798025859383	0.0
16	0.0	0.0	0.0	0.043896569353997425	0.0
17	0.0	0.0	0.0	0.05026587549555784	0.0
18	0.0	0.0	0.0	0.052848026634028274	0.0
19	0.0	0.0	0.0	0.05715161186481234	0.0
20	0.0	0.0	0.0	0.06334877459714139	0.0
21	0.0	0.0	0.0	0.07402166596948585	0.0
22	0.0	0.0	0.0	0.08968671620953984	0.0
23	0.0	0.0	0.0	0.12325468100965552	0.0
24	0.0	0.0	0.0	0.17162697900366838	0.0
25	0.0	0.0	0.0	0.18453773469602056	0.0
26	0.0	0.0	0.0	0.19813706402529818	0.0
27	0.0	0.0	0.0	0.21535140494843444	0.0
28	0.0	0.0	0.0	0.2508129472500951	0.0
29	0.0	0.0	0.0	0.31433386525646784	0.0
30	0.0	0.0	0.0	0.4325963873984139	0.0
31	0.0	0.0	0.0	0.7634560199410926	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	30	8.304905E-6	37.0	36
CGCCGAA	30	3.597306E-4	30.833334	33
TCTTGCG	25	0.0054941326	29.6	2
AGGACGG	40	5.9351478E-5	27.750002	5
GCCGAAT	35	8.864093E-4	26.42857	34
ACGGATC	50	9.080983E-6	25.900002	8
GGACGGA	50	9.080983E-6	25.900002	6
GCAGCGT	65	9.3750714E-8	25.615385	1
CAGCGTC	60	1.3355038E-6	24.666668	2
AGCGTCA	60	1.3355038E-6	24.666668	3
GTCTATT	45	1.3221118E-4	24.666666	2
TAGGACG	45	1.3221118E-4	24.666666	4
TACACTT	45	1.3221118E-4	24.666666	5
AGACGAA	55	1.9005594E-5	23.545454	15
CTTATCC	55	1.9005594E-5	23.545454	3
TATACTG	55	1.9005594E-5	23.545454	5
GGTATCA	2815	0.0	23.461811	1
CATGCGC	40	0.0019300431	23.125002	29
GCAGTCG	80	2.7199349E-8	23.125002	9
GCGCCGA	40	0.0019300431	23.125002	32
>>END_MODULE