FastQCFastQC Report
Fri 10 Feb 2017
ERR1630630.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630630.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169171
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27801.6433076591141509No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23751.4039049246029165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20681.2224317406647711No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10400.6147625775103298No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4980.2943766957693695No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC4400.26009185971590876No Hit
CTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGC4030.23822049878525278No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.22935373084039226No Hit
TCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAA3220.19033995188300595No Hit
GGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAC3210.18974883402001524No Hit
TGCCTACCCTACGCCGGCAGGCGCGGGTAACCCGTTGAACCCC2940.1737886517192663No Hit
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC2850.16846859095235No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2660.15723735155552665No Hit
CCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTC2620.15487288010356384No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2490.14718834788468474No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT2430.14364164070674051No Hit
GCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGC2360.1395038156658056No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG2270.1341837548988893No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2210.1306370477209451No Hit
GGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTAC2200.13004592985795438No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATG2200.13004592985795438No Hit
ACACTGACTGGCTCAGCGTGTGCCTACCCTACGCCGGCAGGCG2180.12886369413197296No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA2070.12236139763907525No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2060.12177027977608457No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT1980.11704133687215894Illumina PCR Primer Index 11 (95% over 22bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1930.11408574755720544No Hit
GGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGG1900.11231239396823332No Hit
ACCCGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGT1870.11053904037926121No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1830.10817456892729842No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGC1760.1040367438863635No Hit
GACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAACAGGTC1730.1022633902973914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGAA250.00548326629.59999815
ATCTTAC250.00548326629.5999981
GTACTAT250.00548326629.5999981
CGAAGAG250.00548326629.59999818
CATAGGG358.8380306E-426.428572
CCATAGG451.3167057E-424.6666661
GCCGTCT701.9028266E-723.78571336
GGTCTTC400.001924422123.1250027
CCGTCTT753.701898E-722.237
AGGGTCT502.688924E-422.1999995
TGTACTG603.70216E-521.5833325
GCTCGGA603.70216E-521.58333211
GGGCAGT1550.021.4838717
GCAGTCG1600.020.81259
CCCCGCC450.003812535220.5555557
GTGTCAT450.003812535220.55555524
TAGGGTC450.003812535220.5555554
CAGAGGA450.003812535220.5555554
CTGTGTC450.003812535220.55555522
TTCTCGT450.003812535220.55555511
Produced by FastQC (version 0.11.5)